The present study was conducted to investigate the influence of rehydration with diluted sports drink and water gargling on salivary flow, pH and buffering capacity during exercise. Nineteen participants performed a 30-min bicycle ergometer exercise at 80% of maximal heart rate while consuming a sports drink, followed by gargling with water, or no gargling, immediately after the exercise (Exp. 1). The same experiment was then conducted on 9 participants who drank 1.5-fold diluted sports drink (Exp. 2-A) and 10 who drank 2-fold diluted sports drink (Exp. 2-B). Paraffin-stimulated whole saliva samples were collected for 3 min before, during and after exercise, and salivary parameters were examined using a hand-held pH meter. Statistical significance was assessed using multi-factor ANOVA and Turkey-Kramer test (P < 0.05). The decrease in salivary pH was significantly suppressed after water gargling in Exp. 1 (P < 0.01) and Exp. 2-A (P < 0.001). Dilution of the sports drink had an interactive effect with water gargling in Exp. 2-B (P < 0.01). These results suggest that gargling with water suppresses the decrease of salivary pH resulting from sports drink rehydration during ergometer exercise, but that this effect may be lost if athletes consume sports drink that has been diluted 2-fold with water.