Identification of Venturia inaequalis Races in Morioka, Japan and Identification of a Quantitative Trait Locus Associated with Resistance to Apple Scab in ‘Akane’ Apples

Shigeki Moriya; Satoshi Goto; Takashi Kubo; Miyuki Kunihisa; Junko Tazawa; Tsuyoshi Kudo; Satoshi Kasai; Haruka Kudo; Kazuma Okada; Toshiya Yamamoto; Tomoko Fukazawa-Akada; Yoshimichi Hatsuyama; Kazuyuki Abe

doi:10.2503/hrj.18.349

Breeding & Germplasm Resources

Identification of Venturia inaequalis Races in Morioka, Japan and Identification of a Quantitative Trait Locus Associated with Resistance to Apple Scab in ‘Akane’ Apples

Shigeki Moriya, Satoshi Goto, Takashi Kubo, Miyuki Kunihisa, Junko Tazawa, Tsuyoshi Kudo, Satoshi Kasai, Haruka Kudo, Kazuma Okada, Toshiya Yamamoto, Tomoko Fukazawa-Akada, Yoshimichi Hatsuyama, Kazuyuki Abe

Author information

Shigeki Moriya
Apple Research Station, Institute of Fruit Tree and Tea Science, NARO
Satoshi Goto
Apple Research Institute, Aomori Prefectural Industrial Technology Research Center
Takashi Kubo
Apple Research Institute, Aomori Prefectural Industrial Technology Research Center
Miyuki Kunihisa
Institute of Fruit Tree and Tea Science, NARO
Junko Tazawa
Apple Research Institute, Aomori Prefectural Industrial Technology Research Center
Tsuyoshi Kudo
Apple Research Institute, Aomori Prefectural Industrial Technology Research Center
Satoshi Kasai
Apple Research Institute, Aomori Prefectural Industrial Technology Research Center
Haruka Kudo
Apple Research Institute, Aomori Prefectural Industrial Technology Research Center
Kazuma Okada
Apple Research Station, Institute of Fruit Tree and Tea Science, NARO
Toshiya Yamamoto
Institute of Fruit Tree and Tea Science, NARO
Tomoko Fukazawa-Akada
Apple Research Institute, Aomori Prefectural Industrial Technology Research Center
Yoshimichi Hatsuyama
Apple Research Institute, Aomori Prefectural Industrial Technology Research Center
Kazuyuki Abe
Apple Research Station, Institute of Fruit Tree and Tea Science, NARO

Keywords: breeding objective, disease resistance breeding, environmental effect, quantitative trait locus analysis, SNP array

JOURNAL FREE ACCESS

2019 Volume 18 Issue 4 Pages 349-361

DOI https://doi.org/10.2503/hrj.18.349

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Abstract

Apple scab, which is caused by Venturia inaequalis (Cooke) G. Wint., is a destructive disease that affects apples (Malus × domestica Borkh.). To develop an approach to breeding apples for scab resistance, race distribution of V. inaequalis in Morioka, Japan and genetic factors affecting partial scab resistance observed on ‘Akane’ were assessed. An inoculation test using several differential hosts suggested that race 1 of V. inaequalis was the predominant race in Morioka, Japan. To characterize the genetic profile of ‘Akane’ resistance, quantitative trait locus (QTL) analyses that employed two years of natural scab infection scores in a fungicide-free orchard in Kuroishi, Aomori, Japan were performed using an F₁ population derived from the ‘Orin’ × ‘Akane’ cross. A QTL allele that explained 20% of the phenotypic variance in scab resistance was detected on chromosome 17 of ‘Akane’. In the middle region of chromosome 17 of ‘Orin’ and in very close proximity to the ‘Akane’ QTL, a weak QTL allele was also detected, which explained 5% of the phenotypic variance observed. We considered that these effects were caused by alleles of the identical QTL. Haplotyping analysis indicated that this QTL resistance allele from ‘Akane’ originated from ‘Worcester Pearmain’.

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