For a better user experience please update your browser or use Chrome or Firefox browser.
Utilities
Publication detail
HomeInterlaboratory comparison study of the colony forming efficiency assay for assessing cytotoxicity of nanomaterials
Interlaboratory comparison study of the colony forming efficiency assay for assessing cytotoxicity of nanomaterials
Publication metadata
Nanotechnology has gained importance in the past years as it provides opportunities for industrial growth and innovation. However, the increasing use of manufactured nanomaterials (NMs) in a number of commercial applications and consumer products raises also safety concerns and questions regarding potential unintended risks to humans and the environment. Since several years the European Commission’s Joint Research Centre (JRC) is putting effort in the development, optimisation and harmonisation of in vitro test methods suitable for screening and hazard assessment of NMs. Work is done in collaboration with international partners, in particular the Organisation for Economic Co-operation and Development (OECD). This report presents the results from an interlaboratory comparison study of the in vitro Colony Forming Efficiency (CFE) cytotoxicity assay performed in the frame of OECD's Working Party of Manufactured Nanomaterials (WPMN). Twelve laboratories from European Commission, France, Italy, Japan, Poland, Republic of Korea, South Africa and Switzerland participated in the study coordinated by JRC. The results show that the CFE assay is a suitable and robust in vitro method to assess cytotoxicity of NMs. The assay protocol is well defined and is easily and reliably transferable to other laboratories. The results obtained show good intra and interlaboratory reproducibility of the assay for both the positive control and the tested nanomaterials. In conclusion the CFE assay can be recommended as a building block of an in vitro testing battery for NMs toxicity assessment. It could be used as a first choice method to define dose-effect relationships for other in vitro assays.
- Published:
- Corporate author(s): Institute for Health and Consumer Protection (Joint Research Centre)
- Personal author(s): Norlén, Hedvig ; Altmeyer, Sandrine ; Andreoli, Cristina ; Bogni, Alessia ; Chevillard, Sylvie ; De Angelis, Isabella ; Chung, Seung-Tae ; Eom, Igchun ; Fujita, Katsuhide ; Gilliland, Douglas ; Grollino, Maria Giuseppa ; Gulumian, Mary ; Hirsch, Cordula ; Ichiraku, Kayo ; Igarashi, Takuya ; Jeong, Jayoung ; Jo, Eunhye ; Kim, Dang-Young ; Kaiser, Jean-Pierre ; Lagache, Doris ; La Spina, Rita ; Lee, Jong Kwon ; Lee, Jieun ; Lovera, Annalisa ; Mäder-Althaus, Xenia ; Nesslany, Fabrice ; Ojea Jimenez, Isaac ; Pacchierotti, Francesca ; Pianella, Francesca ; Paget, Vincent ; Kim, Tae Rim ; Roszak, Joanna ; Rosenkranz, Philipp ; Simar, Sophie ; Stępnik, Maciej ; Vetten, Melissa ; Song, Nam Woong ; Yang, Jun-Young ; Rossi, François ; Kinsner-Ovaskainen, Agnieszka ; Ponti, Jessica
- Themes: Industrial research and development , Consumers' health
- Subject: chemical product , consumer protection , industrial research , innovation , manufactured goods , nanotechnology , research method , research policy , research report , toxic substance
|
PDF
|
|||
| ISSN 1831-9424 | ISBN 978-92-79-44677-1 | DOI 10.2788/406937 | Catalogue number LB-NA-27009-EN-N |
- Released on EU Publications:
Document viewer
Note:
The publication cannot be displayed in the document viewer. Open web version
You may access it from: Download and languages