Effects of anticancer agents on cell viability, proliferative activity and cytokine production of peripheral blood mononuclear cells

Hiromi Sakai; Satoshi Kokura; Takeshi Ishikawa; Reiko Tsuchiya; Manabu Okajima; Tatsuzou Matsuyama; Satoko Adachi; Kazuhiro Katada; Kazuhiro Kamada; Kazuhiko Uchiyama; Osamu Handa; Tomohisa Takagi; Nobuaki Yagi; Yuji Naito; Toshikazu Yoshikawa

doi:10.3164/jcbn.12-60

Original Articles

Effects of anticancer agents on cell viability, proliferative activity and cytokine production of peripheral blood mononuclear cells

Hiromi Sakai, Satoshi Kokura, Takeshi Ishikawa, Reiko Tsuchiya, Manabu Okajima, Tatsuzou Matsuyama, Satoko Adachi, Kazuhiro Katada, Kazuhiro Kamada, Kazuhiko Uchiyama, Osamu Handa, Tomohisa Takagi, Nobuaki Yagi, Yuji Naito, Toshikazu Yoshikawa

Author information

Hiromi Sakai
Department of Gastroenterology and Hepatology, Kyoto Prefectural University of Medicine
Satoshi Kokura
Department of Gastroenterology and Hepatology, Kyoto Prefectural University of Medicine
Department of Cancer ImmunoCell Regulation, Kyoto Prefectural University of Medicine
Takeshi Ishikawa
Department of Gastroenterology and Hepatology, Kyoto Prefectural University of Medicine
Department of Cancer ImmunoCell Regulation, Kyoto Prefectural University of Medicine
Reiko Tsuchiya
Department of Gastroenterology and Hepatology, Kyoto Prefectural University of Medicine
Manabu Okajima
Department of Gastroenterology and Hepatology, Kyoto Prefectural University of Medicine
Tatsuzou Matsuyama
Department of Gastroenterology and Hepatology, Kyoto Prefectural University of Medicine
Satoko Adachi
Department of Cancer ImmunoCell Regulation, Kyoto Prefectural University of Medicine
Kazuhiro Katada
Department of Gastroenterology and Hepatology, Kyoto Prefectural University of Medicine
Kazuhiro Kamada
Department of Gastroenterology and Hepatology, Kyoto Prefectural University of Medicine
Kazuhiko Uchiyama
Department of Gastroenterology and Hepatology, Kyoto Prefectural University of Medicine
Osamu Handa
Department of Gastroenterology and Hepatology, Kyoto Prefectural University of Medicine
Tomohisa Takagi
Department of Gastroenterology and Hepatology, Kyoto Prefectural University of Medicine
Nobuaki Yagi
Department of Gastroenterology and Hepatology, Kyoto Prefectural University of Medicine
Yuji Naito
Department of Gastroenterology and Hepatology, Kyoto Prefectural University of Medicine
Toshikazu Yoshikawa
Department of Cancer ImmunoCell Regulation, Kyoto Prefectural University of Medicine

Keywords: chemoimmunotherapy, 5-fluorouracil (5-FU), irinotecan (CPT-11), cisplatin (CDDP), gemcitabine (GEM)

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2013 Volume 52 Issue 1 Pages 64-71

DOI https://doi.org/10.3164/jcbn.12-60

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Abstract

We investigated the effects of anticancer agents on peripheral blood mononuclear cells for the purpose of providing data to support new translational chemoimmunotherapy regimens. Peripheral-blood mononuclear cells were treated with one of four anticancer agents (5-fluorouracil, irinotecan, cisplatin, and gemcitabine) for 2 h, after which cell viability was determined. For assessment of effects of each drug on proliferation and cytokine production, cells were stimulated with phytohemagglutinin for 48 h. As a result, the anticancer agents did not affect cell viability. Cell proliferation was unaffected by 5-fluorouracil and irinotecan but inhibited by cisplatin and gemcitabine. Treatment with gemcitabine enhanced the production of IFN-γ and decreased the number of regulatory T cells. gemcitabine treatment increased IFN-γ production among CD4 T cells but not among CD8 T cells. The results indicated that GEM had immunoregulatory properties that might support immune response against cancer. This finding has implications for designing chemoimmunotherapy strategies.

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