Japanese Journal of Phytopathology
Online ISSN : 1882-0484
Print ISSN : 0031-9473
ISSN-L : 0031-9473
Molecular Analysis of the Wheat Blast Population in Brazil with a Homolog of Retrotransposon MGR583
Alfredo S. URASHIMAYoko HASHIMOTOLe D. DONMotoaki KUSABAYukio TOSAHitoshi NAKAYASHIKIShigeyuki MAYAMA
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1999 Volume 65 Issue 4 Pages 429-436

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Abstract

We have identified a family of dispersed repetitive DNA sequences in the genome of a Magnaporthe grisea isolate from finger millet (Eleusine coracana). Southern blot analyses showed that this element is present in a moderate copy number (30-40 copies) in the genome of blast isolates from wheat (Triticum aestivum). DNA sequence data suggested that this element contains a region highly homologous to the reverse transcriptase domain of MGR583, a poly A-type retrotransposon. Using the reverse transcriptase domain of this element as a molecular probe, the genetic structure of the wheat blast population was examined. DNA fingerprinting analyses revealed that the wheat-infecting isolates constitute a separate, single lineage of their own, suggesting that they are derived from a single origin.

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