Gamma irradiation is one of the physical mutagens that are widely used for induced mutations, food sterilization and medicinal healing. In the present study, irradiation techniques were applied to investigate the effect of gamma irradiation on seed germination, meiotic behavior and pollen fertility of tomato (Solanum lycopersicum L.). The seeds of Solanum lycopersicum (var. Bharat ratan -221) were irradiated with different doses of gamma rays (10, 15, 20, 25, 30, 35 and 40 kR). Results showed that seed germination and pollen fertility in M1 generation reduced steadily with the increasing doses of gamma rays. During the study, increases in meiotic abnormalities with increasing doses were noticed in mutagenic population of gamma rays in M1 generation. The most common chromosome abnormalities observed include univalents, multivalents, bridges, laggards, fragments, micronuclei and disturbed polarity, etc.