Photosynthetica 2020, 58(SI):479-487 | DOI: 10.32615/ps.2020.005

Special issue in honour of Prof. Reto J. Strasser – Dorsoventral variation in photosynthesis during leaf senescence probed by chlorophyll a fluorescence induction kinetics in cucumber and maize plants

H.Y. WU1,2, F.Q. DONG1, L.A. LIU1, L. SHI1, W.F. ZHANG2, C.D. JIANG1
1 Key Laboratory of Plant Resources/Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, 100093 Beijing, China
2 College of Agriculture, Shihezi University/Key Laboratory of Oasis Ecology Agriculture of Xinjiang Production and Construction Corps, 832003 Shihezi, Xinjiang, China

Although photosynthesis has been intensively studied during leaf senescence, it is unclear whether the changes of photosynthetic function in various mesophyll tissues can be detected using chlorophyll a fluorescence induction kinetics (CFI) in vivo. To clarify this question, leaf structure and CFI were carefully examined in senescent leaves. The results showed declines in chlorophyll content and photosynthetic rate in both cucumber and maize plants as leaves aged. In cucumber leaves, the number of chloroplasts decreased more rapidly in the palisade tissue compared to the sponge tissue as senescence progressed. Under various pulse light intensities, the enhancement of the relative variable fluorescence in the CFI curves measured on the adaxial side was larger than that on the abaxial side of senescent cucumber leaves, indicating senescence in the palisade tissue progressed more quickly than that in the sponge tissue. JIP-test parameters further showed that the loss of photosynthetic electron transport activity in palisade tissue was faster than that in sponge tissue. However, during leaf senescence in maize, there were no such dorsoventral differences. We therefore propose that the decline of photosynthesis in cucumber leaves is largely determined by the senescence of palisade tissue. The changes in photosynthesis in palisade and sponge tissues with leaf aging can be probed effectively by CFI measured on both sides of cucumber leaves in vivo.

Additional key words: dorsiventral leaf; isobilateral leaf; light gradient; microstructure; photosystem II.

Received: September 5, 2019; Revised: December 31, 2019; Accepted: January 16, 2020; Prepublished online: February 14, 2020; Published: May 28, 2020  Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
WU, H.Y., DONG, F.Q., LIU, L.A., SHI, L., ZHANG, W.F., & JIANG, C.D. (2020). Special issue in honour of Prof. Reto J. Strasser – Dorsoventral variation in photosynthesis during leaf senescence probed by chlorophyll a fluorescence induction kinetics in cucumber and maize plants. Photosynthetica, 58(SPECIAL ISSUE), 479-487. doi: 10.32615/ps.2020.005
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    Supplementary files

    Download fileWu 2397 supplement.docx

    File size: 834.73 kB

    References

    1. Arnon D.I.: Copper enzymes in isolated chloroplasts. Polyphenol-oxidase in Beta vulgaris. - Plant Physiol. 24: 1-15, 1949. Go to original source...
    2. Bi H.G., Liu P.P., Jiang Z.S., Ai X.Z.: Overexpression of the rubisco activase gene improves growth and low temperature and weak light tolerance in Cucumis sativus. - Physiol. Plantarum 161: 224-234, 2017. Go to original source...
    3. Borsuk A.M., Brodersen C.R.: The spatial distribution of chlorophyll in leaves. - Plant Physiol. 180: 1406-1417, 2019. Go to original source...
    4. Brouwer B., Gardeström P., Keech O.: In response to partial plant shading, the lack of phytochrome A does not directly induce leaf senescence but alters the fine-tuning of chlorophyll biosynthesis. - J. Exp. Bot. 65: 4037-4049, 2014. Go to original source...
    5. Driscoll S.P., Prins A., Olmos E. et al.: Specification of adaxial and abaxial stomata, epidermal structure and photosynthesis to CO2 enrichment in maize leaves. - J. Exp. Bot. 57: 381-390, 2006. Go to original source...
    6. Evans J.R.: Leaf anatomy enables more equal access to light and CO2 between chloroplasts. - New Phytol. 143: 93-104, 1999. Go to original source...
    7. Evans J.R., Vogelmann T.C.: Photosynthesis within isobilateral Eucalyptus pauciflora leaves. - New Phytol. 171: 771-782, 2006. Go to original source...
    8. Force L., Critchley C., van Rensen J.J.S.: New fluorescence parameters for monitoring photosynthesis in plants. - Photosynth. Res. 78: 17, 2003. Go to original source...
    9. Genty B., Briantais J.M., Baker N.R.: The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. - BBA-Gen. Subjects 990: 87-92, 1989. Go to original source...
    10. Gong Y., Chen H.M., Jiang C.D., Shi L.: [Quantification of leaf anatomical structure and its application in a C4 plant, sorghum.] - Chin. Bull. Bot. 49: 173-182, 2014. [In Chinese] Go to original source...
    11. Holloway P.J., Maclean D.J., Scott K.J.: Rate-limiting steps of electron transport in chloroplasts during ontogeny and senescence of barley. - Plant Physiol. 72: 795-801, 1983. Go to original source...
    12. Hsu B.D., Leu K.: A possible origin of the middle phase of polyphasic chlorophyll fluorescence transient. - Funct. Plant Biol. 30: 571-576, 2003. Go to original source...
    13. Ishizuka M., Makino A., Suzuki Y., Mae T.: Amount of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) protein and levels of mRNAs of rbcS and rbcL in the leaves at different positions in transgenic rice plants with decreased content of Rubisco. - Soil Sci. Plant Nutr. 50: 233-239, 2004. Go to original source...
    14. Jiang C.D., Jiang G.M., Wang X.Z. et al.: Increased photosynthetic activities and thermostability of photosystem II with leaf development of elm seedlings (Ulmus pumila) probed by the fast fluorescence rise OJIP. - Environ. Exp. Bot. 58: 261-268, 2006. Go to original source...
    15. Jiang C.D., Wang X., Gao H.Y. et al.: Systemic regulation of leaf anatomical structure, photosynthetic performance, and high-light tolerance in sorghum. - Plant Physiol. 155: 1416-1421, 2011. Go to original source...
    16. Krieger-Liszkay A., Krupinska K., Shimakawa G.: The impact of photosynthesis on initiation of leaf senescence. - Physiol. Plantarum 166:148-164, 2019. Go to original source...
    17. Krupinska K., Humbeck K.: Photosynthesis and chloroplast breakdown. - In: Noodén L.D. (ed.): Plant Cell Death Processes. Pp. 169-187. Academic Press, San Diego 2004. Go to original source...
    18. Martínez D.E., Costa M.L., Guiamet J.J.: Senescence-associated degradation of chloroplast proteins inside and outside the organelle. - Plant Biol. 10: 15-22, 2008. Go to original source...
    19. Nath K., Phee B.K., Jeong S. et al.: Age-dependent changes in the functions and compositions of photosynthetic complexes in the thylakoid membranes of Arabidopsis thaliana. - Photosynth. Res. 117: 547-556, 2013. Go to original source...
    20. Oda-Yamamizo C., Mitsuda N., Sakamoto S. et al.: The NAC transcription factor ANAC046 is a positive regulator of chlorophyll degradation and senescence in Arabidopsis leaves. - Sci. Rep.-UK 6: 23609, 2016. Go to original source...
    21. Pantaleoni L., Ferroni L., Baldisserotto C. et al.: Photosystem II organisation in chloroplasts of Arum italicum leaf depends on tissue location. - Planta 230: 1019-1031, 2009. Go to original source...
    22. Prakash J.S., Srivastava A., Strasser R.J., Mohanty P.: Senescence-induced alterations in the photosystem II functions of Cucumis sativus cotyledons: Probing of senescence driven alterations of photosystem II by chlorophyll a fluorescence induction OJIP transients. - Indian J. Biochem. Bio. 40: 160-168, 2003. Go to original source...
    23. Soares A.S., Driscoll S.P., Olmos E. et al.: Adaxial/abaxial specification in the regulation of photosynthesis and stomatal opening with respect to light orientation and growth with CO2 enrichment in the C4 species - Paspalum dilatatum. - New Phytol. 177: 186-198, 2008. Go to original source...
    24. Soares-Cordeiro A.S., Driscoll S.P., Pellny T.K. et al.: Variations in the dorso-ventral organization of leaf structure and Kranz anatomy coordinate the control of photosynthesis and asso-ciated signalling at the whole leaf level in monocotyledonous species. - Plant Cell Environ. 32: 1833-1844, 2009. Go to original source...
    25. Strasser R.J., Srivastava A., Tsimilli-Michael M.: The fluores-cence transient as a tool to characterize and screen photosyn-thetic samples. - In: Yunus M., Pathre U., Mohanty P. (ed.): Probing Photosynthesis: Mechanisms, Regulation and Adaptation. Pp. 445-483. Taylor & Francis, London 2000.
    26. Strasser R.J., Tsimilli-Michael M., Srivastava A.: Analysis of the chlorophyll a fluorescence transient. - In: Papageorgiou G.C., Govindjee (ed.): Chlorophyll a Fluorescence: A Signature of Photosynthesis. Advances in Photosynthesis and Respiration. Pp. 321-362. Springer, Dordrecht 2004. Go to original source...
    27. Sun J.D., Nishio J.N., Vogelmann T.C.: Green light drives CO2 fixation deep within leaves. - Plant Cell Physiol. 39: 1020-1026, 1998. Go to original source...
    28. Sušila P., Lazár D., Ilík P. et al.: The gradient of exciting radiation within a sample affects the relative height of J and I steps. - Photosynthetica 42: 161-172, 2004. Go to original source...
    29. Tamary E., Nevo R., Naveh L. et al.: Chlorophyll catabolism precedes changes in chloroplast structure and proteome during leaf senescence. - Plant Direct 3: e00127, 2019. Go to original source...
    30. Terashima I., Inoue Y.: Palisade tissue and spongy tissue chloroplasts in spinach: biochemical and ultrastructural differences. - Plant Cell Physiol. 26: 63-75, 1985.
    31. Tholen D., Pons T.L., Voesenek L.A.C.J., Poorter H.: Ethylene insensitivity results in down-regulation of Rubisco expression and photosynthetic capacity in tobacco. - Plant Physiol. 144: 1305-1315, 2007. Go to original source...
    32. Uzelac B., Janošević D., Simonović A. et al.: Characterization of natural leaf senescence in tobacco (Nicotiana tabacum) plants grown in vitro. - Protoplasma 253: 259-275, 2016. Go to original source...
    33. Vogelmann T.C., Han T.: Measurement of gradients of absorbed light in spinach leaves from chlorophyll fluorescence profiles. - Plant Cell Environ. 23: 1303-1311, 2000. Go to original source...

    Return to the content