Micropropagation of <i>Centella asiatica</i> L. An Important Medicinal Herb

Authors

  • R Das Department of Genetic Engineering and Biotechnology University of Rajshahi, Rajshahi-6205, Bangladesh
  • MF Hasan Department of Genetic Engineering and Biotechnology University of Rajshahi, Rajshahi-6205, Bangladesh
  • MS Hossain Department of Genetic Engineering and Biotechnology University of Rajshahi, Rajshahi-6205, Bangladesh
  • M Rahman Department of Genetic Engineering and Biotechnology University of Rajshahi, Rajshahi-6205, Bangladesh

DOI:

https://doi.org/10.3329/pa.v19i2.16928

Keywords:

Micropropagation, Shoot tip, Centella asiatica

Abstract

High frequencies of multiple shoot regeneration were achieved from shoot tip explants cultured on MS media fortified with 4.0 mgl-1 BAP + 0.1 mgl-1 NAA. On an average 10.2?0.38 shoots per explant were obtained. The regenerated shoots were rooted in MS medium supplemented with 1.0 mgl-1 IBA. The in vitro grown plantlets were acclimatized and successfully transferred to natural condition with 80% survival. A reproducible protocol was established for in vitro propagation through multiple shoot induction of Centella asiatica L. an important medicinal herb having high medicinal value.

DOI: http://dx.doi.org/10.3329/pa.v19i2.16928

Progress. Agric. 19(2): 51 - 56, 2008

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Published

2013-11-09

How to Cite

Das, R., Hasan, M., Hossain, M., & Rahman, M. (2013). Micropropagation of <i>Centella asiatica</i> L. An Important Medicinal Herb. Progressive Agriculture, 19(2), 51–56. https://doi.org/10.3329/pa.v19i2.16928

Issue

Vol. 19 No. 2 (2008)

Section

Crop Science