Comprehensive Influences of Overexpression of a MYB Transcriptor Regulating Anthocyanin Biosynthesis on Transcriptome and Metabolome of Tobacco Leaves
Abstract
:1. Introduction
2. Results
2.1. Phenotype and Anthocyanin Content of Transgenic Line Overexpressing LrAN2
2.2. Chemical Compounds Difference in Transgenic Lines and Wild-Type (WT)
2.3. Differently-Expressed Genes in Transgenic Lines and WT
2.4. Conjoint Analysis of Transcriptome and Metabolome
3. Discussion
3.1. Transcriptome Turbulence Was Relatively Simple and Metabolome Influence Was Extensive
3.2. Overexpression of MYB Transcription Factors Influences Phenylpropanoid Metabolism
3.3. Amino Acid Biosynthesis is Influenced by the Overexpression of MYB Transcription Factor
4. Materials and Methods
4.1. Materials
4.2. Obtaining Transgenic Lines with Overexpression of MYB Transcription Factor
4.3. Anthocyanin Content Measurement
4.4. Analysis of Chemical Contents
4.5. Illumina Sequencing and Data Analysis
4.6. Quantitative Reverse-Transcription-PCR
4.7. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
Abbreviations
4CL | 4-coumarate-CoA ligase |
CHS | chalcone synthase |
CHI | chalcone isomeras |
F3H | flavanone 3-hydroxylase |
F3′H | flavonoid 3-hydroxylase |
F3′5′H | flavonoid 3′,5′-hydroxylase |
DFR | dihydroflavonol 4-reductase |
ANS | anthocyanidin synthase |
OMT | O-methyltransferase |
MBW | MYB-bHLH-WD40 |
KEGG | kyoto encyclopedia of genes and genomes |
MAPK | mitogen-activated protein kinase |
SNARE | soluble N -ethylmaleimide-sensitive fusion protein attachment protein receptors |
AOAC | association of official analytical chemists |
COG | clusters of orthologous groups of proteins |
GO | gene ontology |
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Category | Total Compounds | Differently Accumulated Compounds | Percent (%) | Up-Accumulation Compounds | Down-Accumulation Compounds |
---|---|---|---|---|---|
Anthocyanins | 9 | 7 | 77.78 | 6 | 1 |
Polyphenol | 12 | 8 | 66.67 | 7 | 1 |
Flavonol | 29 | 17 | 58.62 | 12 | 5 |
Flavonoid | 18 | 10 | 55.56 | 8 | 2 |
Flavone | 49 | 27 | 55.10 | 11 | 16 |
Flavanone | 19 | 9 | 47.37 | 7 | 2 |
Phenylpropanoids | 53 | 25 | 47.17 | 18 | 7 |
Lipids | 68 | 31 | 45.59 | 10 | 21 |
Vitamins and derivatives | 15 | 6 | 40.00 | 3 | 3 |
Terpene | 17 | 6 | 35.29 | 2 | 4 |
Organic acids and derivatives | 97 | 34 | 35.05 | 17 | 17 |
Phenolamides | 16 | 5 | 31.25 | 1 | 4 |
Others | 23 | 5 | 21.74 | 1 | 4 |
Amino acid and derivatives | 82 | 16 | 19.51 | 0 | 16 |
Nucleotide and derivates | 47 | 8 | 17.02 | 4 | 4 |
Isoflavone | 6 | 1 | 16.67 | 0 | 1 |
Carbohydrates | 19 | 3 | 15.79 | 1 | 2 |
Alkaloids | 33 | 5 | 15.15 | 1 | 4 |
Indole derivatives | 7 | 1 | 14.29 | 0 | 1 |
Alcohols | 13 | 0 | 0.00 | 0 | 0 |
Quinones | 2 | 0 | 0.00 | 0 | 0 |
Sterides | 5 | 0 | 0.00 | 0 | 0 |
Total | 639 | 224 | 35.05 | 109 | 115 |
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Zong, Y.; Li, S.; Xi, X.; Cao, D.; Wang, Z.; Wang, R.; Liu, B. Comprehensive Influences of Overexpression of a MYB Transcriptor Regulating Anthocyanin Biosynthesis on Transcriptome and Metabolome of Tobacco Leaves. Int. J. Mol. Sci. 2019, 20, 5123. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20205123
Zong Y, Li S, Xi X, Cao D, Wang Z, Wang R, Liu B. Comprehensive Influences of Overexpression of a MYB Transcriptor Regulating Anthocyanin Biosynthesis on Transcriptome and Metabolome of Tobacco Leaves. International Journal of Molecular Sciences. 2019; 20(20):5123. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20205123
Chicago/Turabian StyleZong, Yuan, Shiming Li, Xinyuan Xi, Dong Cao, Zhong Wang, Ran Wang, and Baolong Liu. 2019. "Comprehensive Influences of Overexpression of a MYB Transcriptor Regulating Anthocyanin Biosynthesis on Transcriptome and Metabolome of Tobacco Leaves" International Journal of Molecular Sciences 20, no. 20: 5123. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20205123