The Interaction of lncRNA XLOC-2222497, AKR1C1, and Progesterone in Porcine Endometrium and Pregnancy
Abstract
:1. Introduction
2. Results
2.1. Analysis of lncRNAs and mRNAs in the Endometrium
2.2. Feature Identification of AKR1C1
2.3. Identification of AKR1C1 Gene Promoter Region
2.4. Feature Identification of XLOC-2222497
2.5. The Regulation of XLOC-2222497 on AKR1C1
2.6. Progesterone Measurement and Regulation on XLOC-2222497 and AKR1C1
3. Discussion
4. Materials and Methods
4.1. Ethics Statement
4.2. Animal Sources and Sample Collection
4.3. Library Construction and Sequencing
4.4. Identification of Differentially Expressed Genes (DEGs)
4.5. Quantitative Real-Time PCR for Gene Expression
4.6. Western Blot
4.7. Cell Culture and Transfection
4.8. Immunohistochemistry
4.9. Endocrinologic Measurement
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Gene | Coding/Non-Coding | Coding Score |
---|---|---|
XLOC-2222497 | Non-coding | -0.589266 |
AKR1C1 | Coding | 6.60874 |
NEAT1 | Non-coding | -1.21743 |
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Su, T.; Yu, H.; Luo, G.; Wang, M.; Zhou, C.; Zhang, L.; Hou, B.; Zhang, C.; Liu, M.; Xu, D. The Interaction of lncRNA XLOC-2222497, AKR1C1, and Progesterone in Porcine Endometrium and Pregnancy. Int. J. Mol. Sci. 2020, 21, 3232. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21093232
Su T, Yu H, Luo G, Wang M, Zhou C, Zhang L, Hou B, Zhang C, Liu M, Xu D. The Interaction of lncRNA XLOC-2222497, AKR1C1, and Progesterone in Porcine Endometrium and Pregnancy. International Journal of Molecular Sciences. 2020; 21(9):3232. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21093232
Chicago/Turabian StyleSu, Tao, Haile Yu, Gan Luo, Mengxia Wang, Changfan Zhou, Long Zhang, Bin Hou, Chi Zhang, Min Liu, and Dequan Xu. 2020. "The Interaction of lncRNA XLOC-2222497, AKR1C1, and Progesterone in Porcine Endometrium and Pregnancy" International Journal of Molecular Sciences 21, no. 9: 3232. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21093232