Changes in the Nasal Microbiota of Pigs Following Single or Co-Infection with Porcine Reproductive and Respiratory Syndrome and Swine Influenza A Viruses
Abstract
:1. Introduction
2. Results
2.1. Effects of Single and Viral Co-Infection on Nasal Microbiota Richness
2.2. Description of Nasal Microbiota Communities in Healthy Pigs
2.3. The Nasal Microbiota Community Changes after Viral Infection
2.4. Correlation Analysis of H3N2 Titre and Relative Abundance of Bacterial Species in Nasal Swabs
3. Discussion
4. Materials and Methods
4.1. Challenge and Nasal Swab Samples
4.2. DNA Extraction by TRIzol and Column Purification
4.3. 16S rRNA Gene Amplicon Sequencing
4.4. Microbiota Analysis
4.5. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Bacterial Family | Spearman r Correlation |
---|---|
Enterobacteriaceae | −0.07 |
Moraxellaceae | −0.23 |
Neisseriaceae | 0.61 * |
Rickettsiales, mitochondria | −0.17 |
Prevotellaceae | 0.20 |
Weeksellaceae | 0.38 |
Staphylococcaceae | 0.41 |
Aerococcaceae | 0.56 |
Lactobacillaceae | 0.40 |
Streptococcaceae | 0.27 |
Lachnospiraceae | −0.42 |
Ruminococcaceae | 0.27 |
Veillonellaceae | −0.39 |
Streptophyta, other | −0.28 |
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Chrun, T.; Leng, J.; La Ragione, R.M.; Graham, S.P.; Tchilian, E. Changes in the Nasal Microbiota of Pigs Following Single or Co-Infection with Porcine Reproductive and Respiratory Syndrome and Swine Influenza A Viruses. Pathogens 2021, 10, 1225. https://0-doi-org.brum.beds.ac.uk/10.3390/pathogens10101225
Chrun T, Leng J, La Ragione RM, Graham SP, Tchilian E. Changes in the Nasal Microbiota of Pigs Following Single or Co-Infection with Porcine Reproductive and Respiratory Syndrome and Swine Influenza A Viruses. Pathogens. 2021; 10(10):1225. https://0-doi-org.brum.beds.ac.uk/10.3390/pathogens10101225
Chicago/Turabian StyleChrun, Tiphany, Joy Leng, Roberto M. La Ragione, Simon P. Graham, and Elma Tchilian. 2021. "Changes in the Nasal Microbiota of Pigs Following Single or Co-Infection with Porcine Reproductive and Respiratory Syndrome and Swine Influenza A Viruses" Pathogens 10, no. 10: 1225. https://0-doi-org.brum.beds.ac.uk/10.3390/pathogens10101225