Equisetin Restores Colistin Sensitivity against Multi-Drug Resistant Gram-Negative Bacteria
Abstract
:1. Introduction
2. Results
2.1. Equisetin Synergies with Colistin against MDR Gram-Negative Bacteria
2.2. Mechanism of Equisetin Combined with Colistin against Bacteria
2.3. FIC for Parent and LPS-Deficient A. baumannii Strains
2.4. In Vivo Efficacy of Equisetin Combined with Colistin
3. Discussion
4. Materials and Methods
4.1. Bacterial Isolates
4.2. Drugs
4.3. Antibacterial Test
4.4. FIC Index Determination
4.5. Growth Curves of Bacteria
4.6. Time-Kill Assays
4.7. Antibiotic Accumulation Analysis
4.8. Outer Membrane Permeability Assay
4.9. Cytoplasmic Membrane Permeability Assay
4.10. ROS Measurement
4.11. Reverse Transcription-(RT)PCR Analysis
4.12. G. mellonella Infection Model
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Conflicts of Interest
References
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Equisetin + Colistin (µg/mL) | Inhibition Ratio |
---|---|
1 + 1 | 73.91% (17/23) |
2 + 0.5 | 82.60% (19/23) |
2 + 1 | 91.30% (21/23) |
4 + 0.5 | 95.65% (22/23) |
4 + 1 | 100.00% (23/23) |
A. baumannii Strain | Colistin (µg/mL) | Equisetin (µg/mL) |
---|---|---|
176 (wild type) | 0.25 | >128 |
176 (mutant lacking LPS) | >128 | 1 |
7-2 (wild type) | 0.125 | >128 |
7-2 (mutant lacking LPS) | >128 | 1 |
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Zhang, Q.; Chen, S.; Liu, X.; Lin, W.; Zhu, K. Equisetin Restores Colistin Sensitivity against Multi-Drug Resistant Gram-Negative Bacteria. Antibiotics 2021, 10, 1263. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics10101263
Zhang Q, Chen S, Liu X, Lin W, Zhu K. Equisetin Restores Colistin Sensitivity against Multi-Drug Resistant Gram-Negative Bacteria. Antibiotics. 2021; 10(10):1263. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics10101263
Chicago/Turabian StyleZhang, Qi, Shang Chen, Xiaojia Liu, Wenhan Lin, and Kui Zhu. 2021. "Equisetin Restores Colistin Sensitivity against Multi-Drug Resistant Gram-Negative Bacteria" Antibiotics 10, no. 10: 1263. https://0-doi-org.brum.beds.ac.uk/10.3390/antibiotics10101263