Long-Term Cryostorage of Mesenchymal Stem Cell-Containing Hybrid Hydrogel Scaffolds Based on Fibrin and Collagen
Abstract
:1. Introduction
2. Results and Discussion
3. Conclusions
4. Materials and Methods
4.1. Compliance with Legal Regulations and Ethical Norms
4.2. Hybrid Hydrogel Scaffolds
4.3. Cell Cultures
4.4. Cryostorage of Scaffolds
4.5. Microscopy
4.6. Quantitative Analysis of Cells in Scaffolds
4.7. Statistical Analysis
Author Contributions
Funding
Conflicts of Interest
References
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The Timing of the Cryostorage | Before Freezing (Total Number of Cell Nuclei per 1 mm3 Scaffold) | After Freezing (Total Number of Cell Nuclei per 1 mm3 Scaffold) | ||||
---|---|---|---|---|---|---|
72 h | 144 h | Bb 24 h | Bb 96 h | DMSO 24 h | DMSO 96 h | |
3 months | 354.13 ± 18.16 | 616.53 ± 20.71 ○○ | 548.95 ± 19.21 ○ | 687.18 ± 19.48 ▪ ○ ● | 589.46 ± 26.98 ○○ | 636.07 ± 17.6 ◊ ○○ |
6 months | 338.16 ± 11.20 | 640.90 ± 15.40 ○○ | 513.26 ± 25.15 ○○ | 339.54 ± 18.48 ▪ ○ ● | 510.10 ± 21.89 ○ | 312.17 ± 18.29 ◊◊◊ ○○ ● |
9 months | 346.35 ± 12.03 | 787.91 ± 29.71 ○○ | 517.70 ± 10.76 ○○ | 327.17 ± 8.04 ▪ ○ | 428.76 ± 10.86 ○○ ∆ | 354.64 ± 16.11 ◊◊ ○ ● |
12 months | 369.46 ± 21.40 | 640.90 ± 14.85 ○○ | 587.83 ± 36.97 ○ | 592.82 ± 41.63 | 540.02 ± 21.51 ○ | 539.42 ± 21.78 ○ |
The Timing of the Cryostorage | Before Freezing (Number of Nuclei of Dead Cells per 1 mm3 Scaffold) | After Freezing (Number of Nuclei of Dead Cells per 1 mm3 Scaffold) | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
72 h | % | 144 h | % | Bb 24 h | % | Bb 96 h | % | DMSO 24 h | % | DMSO 96 h | % | |
3 months | 2.48 ± 0.64 | 0.70 | 2.64 ± 0.68 | 0.43 | 4.89 ± 0.94 | 0.89 | 7.99 ± 2.37 ○ | 1.16 | 4.58 ± 1.44 | 0.78 | 10.32 ± 2.09 ○ ● | 1.62 |
6 months | 1.24 ± 0.32 | 0.37 | 4.04 ± 1.04 ○ | 0.63 | 2.43 ± 1.70 | 0.47 | 4.38 ± 2.34 | 1.29 | 1.95 ± 0.86 | 0.38 | 3.89 ± 2.12 | 1.25 |
9 months | 2.56± 0.66 | 0.74 | 7.06 ± 1.82 | 0.90 | 43.59 ± 3.07 ○○ | 8.42 | 47.83 ± 7.44 ○○ | 14.62 | 50.30 ± 7.04 ○○ ● | 11.73 | 51.79 ± 9.94 ○○ ● | 14.60 |
12 months | 1.71 ± 0.44 | 0.46 | 3.82 ± 0.98 ○ | 0.59 | 205.35 ± 23.24 ○○ | 34.93 | 235.52 ± 27.75 ○○ | 39.73 | 241.36 ± 37.74 ○○ ●● | 44.69 | 264.2 ± 28.94 ○○ ●● | 48.99 |
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Egorikhina, M.N.; Rubtsova, Y.P.; Aleynik, D.Y. Long-Term Cryostorage of Mesenchymal Stem Cell-Containing Hybrid Hydrogel Scaffolds Based on Fibrin and Collagen. Gels 2020, 6, 44. https://0-doi-org.brum.beds.ac.uk/10.3390/gels6040044
Egorikhina MN, Rubtsova YP, Aleynik DY. Long-Term Cryostorage of Mesenchymal Stem Cell-Containing Hybrid Hydrogel Scaffolds Based on Fibrin and Collagen. Gels. 2020; 6(4):44. https://0-doi-org.brum.beds.ac.uk/10.3390/gels6040044
Chicago/Turabian StyleEgorikhina, Marfa N., Yulia P. Rubtsova, and Diana Ya. Aleynik. 2020. "Long-Term Cryostorage of Mesenchymal Stem Cell-Containing Hybrid Hydrogel Scaffolds Based on Fibrin and Collagen" Gels 6, no. 4: 44. https://0-doi-org.brum.beds.ac.uk/10.3390/gels6040044