Species-Specific Glucose-6-Phosphatase Activity in the Small Intestine—Studies in Three Different Mammalian Models
Abstract
:1. Introduction
2. Results
2.1. Activity of G6Pase System in the Liver and Intestinal Microsomes of Different Species
2.2. Expression of the Components of G6Pase System in Liver and Intestinal Microsomes of Different Species
2.3. The G6Pase System of the Small Intestine Is Intraluminally Oriented
2.4. F6P-Dependent Glucose Production in Liver and Intestinal Microsomes of Different Species
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Real-Time RT-PCR
4.3. Preparation of Microsomal Fractions
4.4. Measurement of Protein Concentration
4.5. Western Blot
4.6. Measurement of Glucose Production
4.7. Measurement of Phosphate Production
4.8. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
ER | Endoplasmic reticulum |
F6P | Fructose-6-phosphate |
G6P | Glucose-6-phosphate |
G6Pase | Glucose-6-phosphatase |
G6PC | Glucose-6-phosphatase-α |
G6PC2 | Glucose-6-phosphatase-related protein |
G6PC3 | Glucose-6-phosphatase-β |
G6PT | Glucose-6-phosphate transporter |
IGRP | Glucose-6-phosphatase-related protein |
M6P | Mannose-6-phosphate |
Pi | Inorganic phosphate |
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Varga, V.; Murányi, Z.; Kurucz, A.; Marcolongo, P.; Benedetti, A.; Bánhegyi, G.; Margittai, É. Species-Specific Glucose-6-Phosphatase Activity in the Small Intestine—Studies in Three Different Mammalian Models. Int. J. Mol. Sci. 2019, 20, 5039. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20205039
Varga V, Murányi Z, Kurucz A, Marcolongo P, Benedetti A, Bánhegyi G, Margittai É. Species-Specific Glucose-6-Phosphatase Activity in the Small Intestine—Studies in Three Different Mammalian Models. International Journal of Molecular Sciences. 2019; 20(20):5039. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20205039
Chicago/Turabian StyleVarga, Viola, Zsófia Murányi, Anita Kurucz, Paola Marcolongo, Angelo Benedetti, Gábor Bánhegyi, and Éva Margittai. 2019. "Species-Specific Glucose-6-Phosphatase Activity in the Small Intestine—Studies in Three Different Mammalian Models" International Journal of Molecular Sciences 20, no. 20: 5039. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms20205039