Disruption of the Complex between GAPDH and Hsp70 Sensitizes C6 Glioblastoma Cells to Hypoxic Stress
Abstract
:1. Introduction
2. Results
2.1. Pro-Tumor Effects of CoCl2 in Rat Glioma Cells
2.2. Hypoxia Causes Increased Synthesis and Aggregation of GAPDH
2.3. GAPDH–Hsp70 Interaction May Be Disrupted by AEAC Compound
2.4. The Role of Hsp70-Mediated Chaperoning of GAPDH in Cells Subjected to Hypoxia
2.5. The Effect of GAPDH on the Glioma Tumor Progression in Animal Model
3. Discussion
4. Materials and Methods
4.1. Cells and Chemicals
4.2. Proliferation and Migration Assays
4.3. Colony Formation Assay
4.4. Wound Healing Assay
4.5. RNA Isolation and Real-Time PCR
4.6. Analysis of Aggregates
4.7. Protein–Protein Interaction Assay
4.8. Immunoprecipitation
4.9. Animals
4.10. Statistics
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Mikeladze, M.A.; Dutysheva, E.A.; Kartsev, V.G.; Margulis, B.A.; Guzhova, I.V.; Lazarev, V.F. Disruption of the Complex between GAPDH and Hsp70 Sensitizes C6 Glioblastoma Cells to Hypoxic Stress. Int. J. Mol. Sci. 2021, 22, 1520. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22041520
Mikeladze MA, Dutysheva EA, Kartsev VG, Margulis BA, Guzhova IV, Lazarev VF. Disruption of the Complex between GAPDH and Hsp70 Sensitizes C6 Glioblastoma Cells to Hypoxic Stress. International Journal of Molecular Sciences. 2021; 22(4):1520. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22041520
Chicago/Turabian StyleMikeladze, Marina A., Elizaveta A. Dutysheva, Victor G. Kartsev, Boris A. Margulis, Irina V. Guzhova, and Vladimir F. Lazarev. 2021. "Disruption of the Complex between GAPDH and Hsp70 Sensitizes C6 Glioblastoma Cells to Hypoxic Stress" International Journal of Molecular Sciences 22, no. 4: 1520. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22041520