Core RNA Interference Genes Involved in miRNA and Ta-siRNA Biogenesis in Hops and Their Expression Analysis after Challenging with Verticillium nonalfalfae
Abstract
:1. Introduction
2. Results
2.1. Characterization and Structural Analysis of RNAi Genes
2.2. Phylogenetic Analysis
2.3. Differential Expression of RNAi Genes
3. Discussion
4. Materials and Methods
4.1. Identification of RNAi Genes
4.2. Phylogenetic Analysis
4.3. Hop Inoculation Experiment
4.4. Total RNA Isolation and qPCR Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Protein | Number of Introns | Transcript Length (nt) | CDS Length (aa) | pI | Mw (Da) |
---|---|---|---|---|---|
DCL1 | 19 | 6497 | 1984 | 6.03 | 222,623.18 |
DCL4 | 24 | 5281 | 1645 | 6.14 | 184,947.75 |
AGO1 | 21 | 4519 | 1035 | 9.22 | 114,542.48 |
AGO2 | 2 | 4333 | 1038 | 9.41 | 115,025.66 |
AGO7 | 2 | 3485 | 1029 | 9.33 | 117,545.61 |
RDR2 | 3 | 3743 | 941 | 7.03 | 106,793.04 |
RDR6 | 2 | 4270 | 1204 | 6.66 | 137,281.43 |
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Kunej, U.; Jakše, J.; Radišek, S.; Štajner, N. Core RNA Interference Genes Involved in miRNA and Ta-siRNA Biogenesis in Hops and Their Expression Analysis after Challenging with Verticillium nonalfalfae. Int. J. Mol. Sci. 2021, 22, 4224. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22084224
Kunej U, Jakše J, Radišek S, Štajner N. Core RNA Interference Genes Involved in miRNA and Ta-siRNA Biogenesis in Hops and Their Expression Analysis after Challenging with Verticillium nonalfalfae. International Journal of Molecular Sciences. 2021; 22(8):4224. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22084224
Chicago/Turabian StyleKunej, Urban, Jernej Jakše, Sebastjan Radišek, and Nataša Štajner. 2021. "Core RNA Interference Genes Involved in miRNA and Ta-siRNA Biogenesis in Hops and Their Expression Analysis after Challenging with Verticillium nonalfalfae" International Journal of Molecular Sciences 22, no. 8: 4224. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22084224