Next Article in Journal
Is There Association between Altered Adrenergic System Activity and Microvascular Endothelial Dysfunction Induced by a 7-Day High Salt Intake in Young Healthy Individuals
Next Article in Special Issue
The Road to Healthy Ageing: What Has Indonesia Achieved So Far?
Previous Article in Journal
Examination of Weight-Loss Motivators and Family Factors in Relation to Weight Management Strategies and Dietary Behaviors among Adolescents with Obesity
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Nutrients
Volume 13
Issue 5
10.3390/nu13051730
nutrients-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles thumb_up
...
Endorse textsms
...
Comment
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Dietary Intake of Vitamin E and Fats Associated with Sarcopenia in Community-Dwelling Older Japanese People: A Cross-Sectional Study from the Fifth Survey of the ROAD Study

by
Yuta Otsuka
1,2,*,
Toshiko Iidaka
2,
Chiaki Horii
3,
Shigeyuki Muraki
2,
Hiroyuki Oka
4,
Kozo Nakamura
5,
Takayuki Izumo
1,
Tomohiro Rogi
1,
Hiroshi Shibata
1,
Sakae Tanaka
3 and
Noriko Yoshimura
2
1
Institute for Health Care Science, Suntory Wellness Limited, 8-1-1 Seikadai, Seika-cho, Soraku-gun, Kyoto 619-0284, Japan
2
Department of Preventive Medicine for Locomotive Organ Disorders, 22nd Century Medical & Research Center, The University of Tokyo Hospital, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-8655, Japan
3
Department of Orthopaedic Surgery, Sensory and Motor System Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo 113-8655, Japan
4
Department of Medical Research and Management for Musculoskeletal Pain, 22nd Century Medical and Research Center, The University of Tokyo, Tokyo 113-8655, Japan
5
Towa Hospital, Tokyo 120-0003, Japan
*
Author to whom correspondence should be addressed.
Nutrients 2021, 13(5), 1730; https://0-doi-org.brum.beds.ac.uk/10.3390/nu13051730
Submission received: 13 April 2021 / Revised: 12 May 2021 / Accepted: 17 May 2021 / Published: 20 May 2021
(This article belongs to the Special Issue Nutrition and Age-Related Diseases)
Browse Figures
Versions Notes

Abstract

:
Dietary habits are of considerable interest as a modifiable factor for the maintenance of muscle health, especially sarcopenia. The present study aimed to investigate the association between dietary intake and sarcopenia prevalence in community-dwelling Japanese subjects. This cross-sectional study was conducted using data from the fifth survey of the Research on Osteoarthritis/Osteoporosis against Disability (ROAD) study, and 1345 participants (437 men and 908 women) aged ≥60 years were included in the analysis. Sarcopenia was determined by the definition of the Asian Working Group for Sarcopenia established in 2014, and dietary intake was assessed with the brief-type self-administered diet history questionnaire. Overall, 77 subjects (5.7%) were identified as having sarcopenia, 5.0% of men and 6.1% of women. Multiple logistic regression analysis showed that the odds ratios of sarcopenia for the dietary intake of vitamin E (α-tocopherol, 0.14 (CI 0.04–0.49), β-tocopherol (0.24, CI 0.07–0.78), γ-tocopherol (0.28, CI 0.09–0.87), and fats (fat 0.27, CI 0.08–0.96; monounsaturated fatty acids, 0.22, CI 0.07–0.72, polyunsaturated fatty acids, 0.28, CI 0.09–0.89) at the highest quantile were significantly lower compared with those at the lowest quantile. Therefore, higher dietary intakes of vitamin E and fats would be associated with a lower prevalence of sarcopenia.

Graphical Abstract

1. Introduction

Sarcopenia is characterized by an age-related decrease in skeletal muscle mass and the loss of strength and/or physical function [1], and has become a worldwide social issue because sarcopenia induces a high risk of frailty, mobility limitation, and mortality in older populations [2]. The factors associated with sarcopenia are various and complicated, such as aging, body composition, diet, exercise habits, and inflammation status [1,3,4,5,6]. In addition to understanding its pathophysiology, many studies have focused on strategies for the prevention and treatment of sarcopenia. The importance of exercise and physical activity [5,7], as well as nutrition [4], has been well recognized as a modifiable factor for the maintenance of muscle health. There is considerable evidence that clearly shows the benefits of exercise interventions, especially resistance exercise training [8], for preventing and delaying the progression of sarcopenia in older people. However, compared to exercise interventions, the amount of evidence regarding nutrition is less, and there is a need to better understand the association of dietary factors with sarcopenia.
Several reports have shown the protective potential of nutrients for muscular health [9,10,11]. In particular, supplementary and dietary proteins play an important role in the maintenance of muscle mass and strength [9,10]. Proteins and their active metabolites, essential amino acids, exert anabolic effects of skeletal muscle synthesis via mTOR signaling [12] to enhance muscle hypertrophy [13]. In fact, a recent meta-analysis indicated that protein supplementation enhances the increase in lean body mass and hand grip strength [14,15], and high protein intake (1.0 g/kg body weight per day) was associated with better physical performance in older adults [16]. In addition to protein, the dietary intake of other nutrients such as vitamins, fatty acids, and anti-oxidants is also related to the maintenance of muscle function [9,10] through the mechanisms of anti-oxidation and anti-inflammation. However, little is known about the direct association of these nutrients with sarcopenia, the pathological condition with decreases in both muscle mass and function. Recently, Japanese cohort studies suggested that the dietary intake of protein and vitamin D [17] and the specific dietary pattern characterized by Japanese diets [18] were associated with the prevalence of sarcopenia, although a comprehensive analysis of various nutrients in a large population of community-dwelling Japanese subjects has not been documented.
The Research on Osteoarthritis/osteoporosis Against Disability (ROAD) study was a prospective cohort study which aimed to characterize the environmental and genetic backgrounds of musculoskeletal diseases such as osteoarthritis, osteoporosis, sarcopenia, and other locomotor dysfunctions in the Japanese community-dwelling population [19,20,21,22]. The ROAD study started in 2005, and the fifth survey was completed in 2018–2019. The second survey of ROAD in 2008–2010 identified the prevalence of sarcopenia using the definition of the Asian Working Group for Sarcopenia (AWGS) established in 2014 [23] as 8.5% in men and 8.0% in women among the general Japanese population ≥60 years old [21]. The same study showed that the cumulative incidences of sarcopenia increased with age in both men and women [21]. Furthermore, another study from the second ROAD survey indicated the association between the prevalence of sarcopenia and exercise habits [24], although the relationship of dietary intake with sarcopenia has not been clarified.
In the present study, data from the fifth survey of the ROAD study in which an examination of sarcopenia was conducted were examined. The aim of this study was to investigate the cross-sectional association between dietary intake and sarcopenia prevalence in community-dwelling Japanese subjects using the AWGS criteria from 2014, as well as the previous study from the second ROAD survey [21].

2. Materials and Methods

2.1. Study Participants

The data for the present study were obtained from the fifth survey of the ROAD study. The ROAD study was a prospective study of bone and joint diseases that consisted of population-based cohorts from several regions in Japan established in 2005. The details of the cohort profiles have been reported previously [19,20,21,22]. Briefly, between 2005 and 2007, a baseline database was created including clinical and genetic information for 3040 residents (1061 men and 1979 women). The subjects were recruited from resident registration lists in 3 regions from an urban region in Itabashi, Tokyo, a mountainous region in Hidakagawa, Wakayama, and a coastal region in Taiji, Wakayama.
The fifth survey of the ROAD study was performed only in the cohorts from the mountainous and coastal regions, from 2018 to 2019. In the fifth survey, the diagnosis of sarcopenia, including measurements of usual walking speed, grip strength, and skeletal muscle mass, was assessed, which was initiated from the second survey in 2008 [21]. In the present study, 2386 participants (768 men and 1618 women) from the mountainous and coastal regions were recruited, and participants aged ≥60 years were selected for analysis based on the AWGS criteria for sarcopenia defined in 2014 [23], as well as the second survey [21]. As described in Figure 1, 1345 (437 men and 908 women; mean age, 71.2 (standard deviation, 7.4) years [71.4 (7.9) years in men, 71.1 (7.1) years in women]) participants were included in the analysis, while 1041 participants were excluded from the analysis because of no visit to the clinic (382 participants), a lack of available data on sarcopenia assessment (13 participants), or being less than 60 years old (646 participants).

2.2. Interviewer-Administered Questionnaire and Anthropometric Measurements

Participants answered an interviewer-administered questionnaire, which included 200 questions concerned with lifestyle, including smoking habits and alcohol consumption. Anthropometric measurements, heights, and weights were also measured, and the body mass index (BMI; weight/height (kg/m2)) was calculated.

2.3. Walking Speed, Muscle Strength, and Skeletal Muscle Mass

For the measurement of walking speed, subjects walked 6 m at a comfortable speed, and the time taken was recorded to calculate the usual walking speed. Handgrip strength was measured with a Toei Light handgrip dynamometer (Toei Light Co. Ltd., Saitama, Japan) to evaluate muscle strength. Both hands were evaluated individually, and the largest values were used to determine the maximum grip strength. Skeletal muscle mass was measured with the Body Composition Analyzer MC-190 (Tanita Corp., Tokyo, Japan) based on bioimpedance analysis [25], whose protocol was described and validated previously [26]. Appendicular skeletal muscle mass was determined as the sum of the arms and legs’ muscle mass, which was converted to an appendicular muscle mass index by dividing by height in meters squared (kg/m2).

2.4. Definition of Sarcopenia

Sarcopenia was determined using the criteria defined by the AWGS in 2014 [23]. The cut-off values of the AWGS criteria were as follows: (A) age ≥60 or ≥65 years; (B) low appendicular skeletal muscle mass, 7.0 kg/m2 for men and 5.7 kg/m2 for women, according to bioimpedance analysis; (C) low handgrip strength, <26 kg in men and <18 kg in women; and (D) low gait speed, with a usual walking speed ≤0.8 m/s. Subjects were diagnosed as having sarcopenia if they fulfilled criteria (A), (B), and (C), or (A), (B), and (D). Regarding the age definition, not all countries use the same cut-off age to define elderly populations in the AWGS criteria due to the different states of aging in Asia. In the present study, subjects aged ≥60 years were defined as potential subjects for sarcopenia, as previously reported in the second ROAD survey [21].

2.5. Dietary Assessment

A self-administered brief diet history questionnaire (BDHQ), which was developed as a short version of the validated self-administered diet history questionnaire in regard to the typical Japanese diet [27] and was widely used for dietary surveys in Japan [28,29], was used. Participants completed the questionnaire at home, and it was then reviewed by well-trained interviewers in the clinic to complete all answers. The BDHQ evaluates the dietary intake frequency of 56 foods and beverage items during the past month to calculate the daily intake of energy and selected nutrients using a specific computer algorithm. In the present study, dietary intake levels of total energy and 51 major nutrient factors were analyzed.

2.6. Statistical Analysis

All statistical analyses were performed using JMP software, version 15.2 (SAS institute Inc, Cary, NC, USA). Differences in characteristics between men and women were compared using Student’s t-test for continuous variables or the chi-squared test for categorical variables. Differences in the dietary intakes of nutrient factors between participants with and without sarcopenia were compared using Student’s t-test. Multiple logistic regression analysis was used to evaluate the associations between the prevalence of sarcopenia and the dietary intakes of nutrient factors. In the analysis, the odds ratio (OR) and 95% confidence intervals (CI) of sarcopenia were calculated for the quintiles of the dietary intake of nutrient factors, using the lowest quintile category as the reference, after adjustment for age, sex, BMI, residence area, current smoking habit, current alcohol drinking habit, and total caloric intake. Trend associations were assessed by entering dummy variables assigned to the quintile of the dietary intake of the nutrient factors. p values < 0.05 were regarded as significant.

3. Results

3.1. Baseline Characteristics of the Study Population

The participants aged ≥60 years for the analysis consisted of 437 men (32.5%) and 908 women (67.5%), as shown in Table 1. There were no significant differences in age and residence area, whereas height, weight, BMI, current smoking habit, and current alcohol drinking habit were higher in men than in women. Among the indices of sarcopenia, grip strength (39.6 kg vs. 24.0 kg) and appendicular muscle mass index (7.7 kg/m2 vs. 6.0 kg/m2) were higher in men than women, but no significant difference was observed in usual walking speed (1.16 m/s vs. 1.18 m/s). In total, 77 subjects (5.7%) were identified as having sarcopenia, and there were no significant differences in the prevalence of sarcopenia between men (5.0%) and women (6.1%). According to age group stratifications of 60–64, 65–69, 70–74, 75–79, and ≥80 years, the prevalences of sarcopenia were 0.0, 0.9, 4.9, 3.8, and 25.2%, respectively (in men, 0.0, 1.7, 2.4, 2.9, and 21.1%; in women, 0.0, 0.4, 5.9, 4.1, and 27.8%, for 60–64, 65–69, 70–74, 75–79, and ≥80 years, respectively).

3.2. Differences between Intakes of Nutrients with and without Sarcopenia

There were no significant differences in total energy between participants with and without sarcopenia (p > 0.05, Table 2). However, among 51 major nutrient factors, the intakes of fat (p = 0.032), vegetable fat (p = 0.013), α-tocopherol (p = 0.049), β-tocopherol (p = 0.004), γ-tocopherol (p = 0.003), δ-tocopherol (p = 0.033), monounsaturated fatty acid (p = 0.016), polyunsaturated fatty acid (p = 0.014), and n-6 fatty acid (p = 0.004) were significantly lower in participants with sarcopenia than in those without sarcopenia (Table 2).

3.3. The Prevalence of Sarcopenia with the Amount of Vitamin E and Fats Intake

Next, the ORs of sarcopenia for quantiles of the nutrient factors for which significant differences between participants with and without sarcopenia were found were analyzed using the lowest quantile category as the reference (Table 3). The dietary intakes of α-, β-, and γ-tocopherol; fat; and monounsaturated and polyunsaturated fatty acids showed inverse associations, but δ-tocopherol, vegetable fat, and n-6 fatty acid were not significant. Adjusted for age, sex, BMI, residence area, current smoking habit, current alcohol drinking habit, and total caloric intake, the ORs for α-, β-, and γ-tocopherol at the fourth quintile were significantly lower compared with the reference values; in particular, the OR for α-tocopherol was the lowest value (OR 0.14; 95% CI 0.04–0.49), compared to β- and γ-tocopherol (OR 0.24; 95% CI 0.07–0.78; OR 0.28; 95% CI 0.09–0.87). Furthermore, among the categories of major nutrients, fat showed a significant association with a lower OR for sarcopenia (OR 0.27; 95% CI 0.08–0.96), and the ORs for monounsaturated and polyunsaturated fatty acids at the fourth quantile were also significantly lower compared with the reference value (OR 0.22; 95% CI 0.07–0.72; OR 0.28; 95% CI 0.09–0.89).

4. Discussion

In the present study, the prevalence of sarcopenia in the fifth survey of the ROAD study in 2018–2019 and differences in the dietary intakes of nutrient factors between participants with and without sarcopenia were examined. To the best of our knowledge, the present study is the first to demonstrate that higher dietary intakes of vitamin E and fats are associated with a low prevalence of sarcopenia in a community-dwelling older population.
The prevalence of sarcopenia in the present results was estimated using the AWGS criteria established in 2014, as well as the previous report from the second ROAD survey [21]. Compared with the second survey in 2008–2009, the prevalence in the fifth survey in 2018- 2019 was significantly lower (in the fifth survey, 5.7% [5.0% in men, 6.1% in women], Table 1; in the second survey, 8.2% [8.5% in men, 8.0% in women, p < 0.05]), especially in the 75–79 year age group (in the fifth survey, 0.0, 0.9, 4.9, 3.8, and 25.2%; in the second survey, 0.5, 0.0, 4.3, 11.2, and 27.0% for 60–64, 65–69, 70–74, 75–79, and ≥80 years, respectively). The reasons for these differences are related to the improvement of the physical function of elderly Japanese persons during the past decade because of increased exercise habits [30]. The prevalence was also estimated using the recently established criteria of AWGS in 2019 [31], whose value was 6.2% [5.9% in men, 6.4% in women]. However, similar trends as with AWGS2014, but no significant associations between its prevalence and the dietary intake of nutrient factors, were observed (data not shown). The cut-off values of the AWGS2014 criteria were lower for usual walking speed (≤0.8 m/s vs. <1.0 m/s) and grip strength in men (<26 kg vs. <28 kg) compared to those of AWGS2019 [31], resulting in lower statistical power for identifying significant associations among subjects with relatively mild symptoms.
Vitamin E includes four types of tocopherols (α, β, γ, and δ), whose main dietary sources are plant oils, nuts, seeds, fish, shellfish, and vegetables. Among them, α-tocopherol is the most abundant in the human body [32], and has various biological functions, such as decreasing lipid peroxide production and the maintenance of membrane fluid [33,34]. A recent review also showed the potential of vitamin E in skeletal muscle health [35], whose mechanism was related to the attenuation of oxidative stress and inflammation in age-associated muscle dysfunction [36,37,38]. In fact, some observational studies suggested that a low concentration of plasma α-tocopherol would be associated with decreased muscle strength and physical function [39,40], and the dietary intake of vitamin E was positively correlated with skeletal muscle mass [40,41], although there was no information about the relationship between its dietary intake and sarcopenia. The present study was the first to report that a higher dietary intake of vitamin E (α-, β-, and γ-tocopherol) was significantly associated with lower ORs of sarcopenia prevalence (Table 3). According to the dietary reference intake defined by the Ministry of Health, Labour and Welfare in Japan, the adequate intake levels of vitamin E, equal to α-tocopherol, are 6.5 and 6.0 mg/day in adult men and women, respectively [42], which corresponded with the values between the first and second quantiles in the present results. Therefore, higher intakes of vitamin E, rather than those of adequate levels, would prevent sarcopenia symptoms.
In the present study, there was a significant association between lower ORs for sarcopenia prevalence and higher intakes of fat, monounsaturated fatty acids, and polyunsaturated fatty acids (Table 3). The relationship between sarcopenia and fat intake has been controversial, because some previous studies have shown that a dietary pattern with a lower percentage of energy from fat was negatively related to the risk of sarcopenia [43,44], but other studies have shown that greater daily fat intake was positively related with skeletal muscle mass and lower sarcopenia prevalence [45,46]. However, the supplementation of specific fatty acids, such as omega-3 fatty acids, increases muscle mass and physical function in elderly people [47]. Furthermore, it has been reported that the plasma fatty acid profile modified by diet and exercise contributes to preventing sarcopenia [48]. Taken together, the dietary intake of fatty acids would provide a beneficial effect on sarcopenia, which is dependent on the type of fatty acid and the characteristics of the subjects. In fact, the dietary intake of oleic acid (C18:1 (M)) also showed a similar association with the OR for sarcopenia prevalence as fat in the present study (data not shown). Oleic acid, well-known as one of the omega-9 fatty acids, is contained mainly in olive oil, and has a protective role in cardiovascular disease [49]. Oleic acid has been reported to prevent muscle atrophy and increase muscle differentiation through the reduction of mitochondrial reactive oxygen species in vitro [50,51], but there have been few reports of clinical studies. Thus, further studies are needed to clarify the beneficial effect of oleic acid in sarcopenia.
The role of dietary protein in sarcopenia is well-known, and several previous studies have suggested positive associations with muscle mass and function [9,10]. However, there was no significant association between protein intake and sarcopenia prevalence in the present study (Table 2). In agreement with the present findings, some observational studies have found no association between the amounts of daily protein intake and sarcopenia or frailty, because the average daily protein intake was relatively high compared to the 0.83 g/kg BW/day recommended by the World Health Organization [52,53], and these associations would be dependent on the definition of sarcopenia [54].
There were some limitations to the present study. First, it was not possible to demonstrate a causal relationship due to the cross-sectional analysis. However, the relationship can be explained by the possibility that higher dietary intakes of vitamin E and fats may prevent the onset of sarcopenia; thus, further longitudinal analyses with 10 years of observation between the second and fifth ROAD surveys are needed to clarify these relationships in the future. Second, individuals who were able to participate in this survey from the mountainous and coastal regions may not be completely representative of the general population in Japan, although a large number of participants were enrolled in the ROAD study. Comparing the anthropometric measurements between the present study participants and the general Japanese population based on the 2018 National Health and Nutrition Survey in Japan [55], there was a significant difference in the mean BMI (22.7 (3.4) vs. 23.4 (0.6) kg/m2, p < 0.001) in subjects aged ≥60 years. Moreover, the proportion of current smokers in the present study was significantly lower in both men and women compared to the general Japanese population (men, 16.5% vs. 22.3%, p < 0.01; women, 2.4% vs. 4.9%, p < 0.01), as well as the proportion of current drinkers (men, 66.6% vs. 71.9%, p < 0.05; women, 28.0% vs. 35.8%, p < 0.001). These findings suggested that the participants in the present study had healthier lifestyles, at least regarding smoking and drinking habits, compared to the general Japanese population, leading to selection bias, which should be taken into consideration when the results in the present study are generalized. Third, the BDHQ was administered for dietary assessment, but this questionnaire has the disadvantages of low accuracy and being susceptible to seasonal effects. However, the BDHQ is considered suitable for investigating dietary habits and has been used in many cohort studies with validation by comparison with dietary records [27]. Furthermore, the strength of the ROAD study was the large population of community-dwelling subjects, and as such, the present results are considered to be highly reflective of general dietary habits.

5. Conclusions

In conclusion, the present study identified the associations between lower sarcopenia prevalence and higher dietary intakes of vitamin E and fats. Further longitudinal studies are needed to clarify the preventive effects of vitamin E and fats on sarcopenia.

Author Contributions

Conceptualization, Y.O., T.I. (Toshiko Iidaka), T.I. (Takayuki Izumo), T.R., H.S. and N.Y.; Data curation, Y.O.; Formal analysis, Y.O.; Funding acquisition, H.O., S.T. and N.Y.; Investigation, T.I. (Toshiko Iidaka), C.H. and N.Y.; Methodology; S.M., H.O., K.N., S.T. and N.Y.; Supervision, N.Y. and Y.O.; Writing—original draft, Y.O.; Writing—review and editing, T.I. (Takayuki Izumo), T.R., H.S. and N.Y. All authors have read and agreed to the published version of the manuscript.

Funding

This work was supported by Grant-in-Aid funding from the Ministry of Health, Labour and Welfare: 19FA1401 (Director, Sakae Tanaka), 19FA0701 (Director, Hiroyuki Oka), and 19FA1901 (Director, Estuo Chosa). The study was also supported by JSPS KAKENHI Grant Number 19H03895 and 18K18447 to Noriko Yoshimura, and JP19K19454 to Toshiko Iidaka. The study was partly supported by grants from the Japan Agency for Medical Research and Development (17dk0110028h0001, Director, Noriko Yoshimura; 17gk0210007h0003, Director, Sakae Tanaka). Further, the study was partly supported by grants from the Mitsui Sumitomo Insurance Welfare Foundation (2016, Director, Noriko Yoshimura), and Japan Dairy Association (2017, Director, Noriko Yoshimura), Nakatomi Foundation (2019, Director, Toshiko Iidaka), and Japan Osteoporosis Foundation (2019, Director, Toshiko Iidaka).

Institutional Review Board Statement

The present study was conducted according to the guidelines of the Declaration of Helsinki and approved by the ethics committees of the University of Tokyo (No. 1264 and 1326) and Wakayama Medical University (No. 373).

Informed Consent Statement

All subjects participating in the ROAD study provided their written, informed consent.

Data Availability Statement

The data are not publicly available due to privacy. The data presented in this study are available in this text.

Acknowledgments

The authors are grateful to all of participants and investigators for their support and cooperation.

Conflicts of Interest

Four of the authors (Y.O., T.I. (Takayuki Izumo), T.R. and H.S.) are salaried employees of Suntory Wellness Limited. All other authors have no conflicts of interest. Y.O., T.I. (Takayuki Izumo), T.R., and H.S. contributed to the interpretation of the data and the revision and approval of the manuscript with the other authors as well. However, this study is not related to any particular products of the company.

References

  1. Cruz-Jentoft, A.J.; Baeyens, J.P.; Bauer, J.M.; Boirie, Y.; Cederholm, T.; Landi, F.; Martin, F.C.; Michel, J.P.; Rolland, Y.; Schneider, S.M.; et al. Sarcopenia: European consensus on definition and diagnosis: Report of the European Working Group on Sarcopenia in Older People. Age Ageing 2010, 39, 412–423. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  2. Cruz-Jentoft, A.J.; Sayer, A.A. Sarcopenia. Lancet 2019, 393, 2636–2646. [Google Scholar] [CrossRef]
  3. Marcos-Pardo, P.J.; González-Gálvez, N.; López-Vivancos, A.; Espeso-García, A.; Martínez-Aranda, L.M.; Gea-García, G.M.; Orquín-Castrillón, F.J.; Carbonell-Baeza, A.; Jiménez-García, J.D.; Velázquez-Díaz, D.; et al. Sarcopenia, Diet, Physical Activity and Obesity in European Middle-Aged and Older Adults: The LifeAge Study. Nutrients 2020, 13, 8. [Google Scholar] [CrossRef] [PubMed]
  4. Bloom, I.; Shand, C.; Cooper, C.; Robinson, S.; Baird, J. Diet Quality and Sarcopenia in Older Adults: A Systematic Review. Nutrients 2018, 10, 308. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  5. Bowen, T.S.; Schuler, G.; Adams, V. Skeletal muscle wasting in cachexia and sarcopenia: Molecular pathophysiology and impact of exercise training. J. Cachexia Sarcopenia Muscle 2015, 6, 197–207. [Google Scholar] [CrossRef] [PubMed]
  6. Vatic, M.; von Haehling, S.; Ebner, N. Inflammatory biomarkers of frailty. Exp. Gerontol 2020, 133, 110858. [Google Scholar] [CrossRef] [PubMed]
  7. Chodzko-Zajko, W.J.; Proctor, D.N.; Fiatarone Singh, M.A.; Minson, C.T.; Nigg, C.R.; Salem, G.J.; Skinner, J.S. American College of Sports Medicine position stand. Exercise and physical activity for older adults. Med. Sci. Sports Exerc. 2009, 41, 1510–1530. [Google Scholar] [CrossRef]
  8. American College of Sports Medicine Position Stand. Progression models in resistance training for healthy adults. Med. Sci. Sports Exerc. 2009, 41, 687–708. [Google Scholar] [CrossRef]
  9. Tessier, A.J.; Chevalier, S. An Update on Protein, Leucine, Omega-3 Fatty Acids, and Vitamin D in the Prevention and Treatment of Sarcopenia and Functional Decline. Nutrients 2018, 10, 1099. [Google Scholar] [CrossRef] [Green Version]
  10. Robinson, S.; Granic, A.; Sayer, A.A. Nutrition and Muscle Strength, As the Key Component of Sarcopenia: An Overview of Current Evidence. Nutrients 2019, 11, 2942. [Google Scholar] [CrossRef] [Green Version]
  11. Mareschal, J.; Genton, L.; Collet, T.H.; Graf, C. Nutritional Intervention to Prevent the Functional Decline in Community-Dwelling Older Adults: A Systematic Review. Nutrients 2020, 12, 2820. [Google Scholar] [CrossRef]
  12. Dreyer, H.C.; Drummond, M.J.; Pennings, B.; Fujita, S.; Glynn, E.L.; Chinkes, D.L.; Dhanani, S.; Volpi, E.; Rasmussen, B.B. Leucine-enriched essential amino acid and carbohydrate ingestion following resistance exercise enhances mTOR signaling and protein synthesis in human muscle. Am. J. Physiol. Endocrinol. Metab. 2008, 294, E392–E400. [Google Scholar] [CrossRef] [Green Version]
  13. Kim, I.Y.; Schutzler, S.; Schrader, A.; Spencer, H.; Kortebein, P.; Deutz, N.E.; Wolfe, R.R.; Ferrando, A.A. Quantity of dietary protein intake, but not pattern of intake, affects net protein balance primarily through differences in protein synthesis in older adults. Am. J. Physiol. Endocrinol. Metab. 2015, 308, E21–E28. [Google Scholar] [CrossRef]
  14. Tagawa, R.; Watanabe, D.; Ito, K.; Ueda, K.; Nakayama, K.; Sanbongi, C.; Miyachi, M. Dose-response relationship between protein intake and muscle mass increase: A systematic review and meta-analysis of randomized controlled trials. Nutr. Rev. 2020, 79, 66–75. [Google Scholar] [CrossRef]
  15. Veronese, N.; Stubbs, B.; Punzi, L.; Soysal, P.; Incalzi, R.A.; Saller, A.; Maggi, S. Effect of nutritional supplementations on physical performance and muscle strength parameters in older people: A systematic review and meta-analysis. Ageing Res. Rev. 2019, 51, 48–54. [Google Scholar] [CrossRef]
  16. Coelho-Júnior, H.J.; Milano-Teixeira, L.; Rodrigues, B.; Bacurau, R.; Marzetti, E.; Uchida, M. Relative Protein Intake and Physical Function in Older Adults: A Systematic Review and Meta-Analysis of Observational Studies. Nutrients 2018, 10, 1300. [Google Scholar] [CrossRef] [Green Version]
  17. Mori, H.; Tokuda, Y. Differences and overlap between sarcopenia and physical frailty in older community-dwelling Japanese. Asia Pac. J. Clin. Nutr. 2019, 28, 157–165. [Google Scholar] [CrossRef]
  18. Suthuvoravut, U.; Takahashi, K.; Murayama, H.; Tanaka, T.; Akishita, M.; Iijima, K. Association between Traditional Japanese Diet Washoku and Sarcopenia in Community-Dwelling Older Adults: Findings from the Kashiwa Study. J. Nutr. Health Aging 2020, 24, 282–289. [Google Scholar] [CrossRef]
  19. Yoshimura, N.; Muraki, S.; Oka, H.; Mabuchi, A.; En-Yo, Y.; Yoshida, M.; Saika, A.; Yoshida, H.; Suzuki, T.; Yamamoto, S.; et al. Prevalence of knee osteoarthritis, lumbar spondylosis, and osteoporosis in Japanese men and women: The research on osteoarthritis/osteoporosis against disability study. J. Bone Min. Metab. 2009, 27, 620–628. [Google Scholar] [CrossRef]
  20. Muraki, S.; Oka, H.; Akune, T.; Mabuchi, A.; En-yo, Y.; Yoshida, M.; Saika, A.; Suzuki, T.; Yoshida, H.; Ishibashi, H.; et al. Prevalence of radiographic knee osteoarthritis and its association with knee pain in the elderly of Japanese population-based cohorts: The ROAD study. Osteoarthr. Cartil. 2009, 17, 1137–1143. [Google Scholar] [CrossRef] [Green Version]
  21. Yoshimura, N.; Muraki, S.; Oka, H.; Iidaka, T.; Kodama, R.; Kawaguchi, H.; Nakamura, K.; Tanaka, S.; Akune, T. Is osteoporosis a predictor for future sarcopenia or vice versa? Four-year observations between the second and third ROAD study surveys. Osteoporos Int. 2017, 28, 189–199. [Google Scholar] [CrossRef] [PubMed]
  22. Yoshimura, N.; Muraki, S.; Iidaka, T.; Oka, H.; Horii, C.; Kawaguchi, H.; Akune, T.; Nakamura, K.; Tanaka, S. Prevalence and co-existence of locomotive syndrome, sarcopenia, and frailty: The third survey of Research on Osteoarthritis/Osteoporosis Against Disability (ROAD) study. J. Bone Min. Metab. 2019, 37, 1058–1066. [Google Scholar] [CrossRef] [PubMed]
  23. Chen, L.K.; Liu, L.K.; Woo, J.; Assantachai, P.; Auyeung, T.W.; Bahyah, K.S.; Chou, M.Y.; Chen, L.Y.; Hsu, P.S.; Krairit, O.; et al. Sarcopenia in Asia: Consensus report of the Asian Working Group for Sarcopenia. J. Am. Med. Dir. Assoc. 2014, 15, 95–101. [Google Scholar] [CrossRef] [PubMed]
  24. Akune, T.; Muraki, S.; Oka, H.; Tanaka, S.; Kawaguchi, H.; Nakamura, K.; Yoshimura, N. Exercise habits during middle age are associated with lower prevalence of sarcopenia: The ROAD study. Osteoporos Int. 2014, 25, 1081–1088. [Google Scholar] [CrossRef]
  25. Janssen, I.; Heymsfield, S.B.; Baumgartner, R.N.; Ross, R. Estimation of skeletal muscle mass by bioelectrical impedance analysis. J. Appl. Physiol. 2000, 89, 465–471. [Google Scholar] [CrossRef] [Green Version]
  26. Nemoto, M.; Yabushita, N.; Kim, M.; Matsuo, T.; Seino, S.; Songee, J.; Sasai, H.; Tanaka, K. Validity of the bioelectrical impedance method for assessing body composition in non-frail and pre-frail older adults. Int. J. Body Compos. Res. 2012, 10, 55–61. [Google Scholar]
  27. Sasaki, S.; Yanagibori, R.; Amano, K. Self-administered diet history questionnaire developed for health education: A relative validation of the test-version by comparison with 3-day diet record in women. J. Epidemiol. 1998, 8, 203–215. [Google Scholar] [CrossRef] [Green Version]
  28. Muraki, S.; Akune, T.; En-yo, Y.; Yoshida, M.; Tanaka, S.; Kawaguchi, H.; Nakamura, K.; Oka, H.; Yoshimura, N. Association of dietary intake with joint space narrowing and osteophytosis at the knee in Japanese men and women: The ROAD study. Mod. Rheumatol. 2014, 24, 236–242. [Google Scholar] [CrossRef]
  29. Odai, T.; Terauchi, M.; Hirose, A.; Kato, K.; Miyasaka, N. Bone Mineral Density in Premenopausal Women Is Associated with the Dietary Intake of α-Tocopherol: A Cross-Sectional Study. Nutrients 2019, 11, 2474. [Google Scholar] [CrossRef] [Green Version]
  30. Japan Sports Agency. The Report of Physical and Exercise Capacity. 2018. Available online: https://www.mext.go.jp/prevsports/comp/bmenu/other/icsFiles/afieldfile/2019/10/15/14219212.pdf (accessed on 25 March 2021).
  31. Chen, L.K.; Woo, J.; Assantachai, P.; Auyeung, T.W.; Chou, M.Y.; Iijima, K.; Jang, H.C.; Kang, L.; Kim, M.; Kim, S.; et al. Asian Working Group for Sarcopenia: 2019 Consensus Update on Sarcopenia Diagnosis and Treatment. J. Am. Med. Dir. Assoc. 2020, 21, 300–307.e302. [Google Scholar] [CrossRef]
  32. Traber, M.G.; Atkinson, J. Vitamin E, antioxidant and nothing more. Free Radic. Biol. Med. 2007, 43, 4–15. [Google Scholar] [CrossRef] [Green Version]
  33. Niki, E. Role of vitamin E as a lipid-soluble peroxyl radical scavenger: In vitro and in vivo evidence. Free Radic. Biol. Med. 2014, 66, 3–12. [Google Scholar] [CrossRef]
  34. Wang, X.; Quinn, P.J. Vitamin E and its function in membranes. Prog. Lipid Res. 1999, 38, 309–336. [Google Scholar] [CrossRef]
  35. Chung, E.; Mo, H.; Wang, S.; Zu, Y.; Elfakhani, M.; Rios, S.R.; Chyu, M.C.; Yang, R.S.; Shen, C.L. Potential roles of vitamin E in age-related changes in skeletal muscle health. Nutr. Res. 2018, 49, 23–36. [Google Scholar] [CrossRef]
  36. Aragno, M.; Mastrocola, R.; Catalano, M.G.; Brignardello, E.; Danni, O.; Boccuzzi, G. Oxidative stress impairs skeletal muscle repair in diabetic rats. Diabetes 2004, 53, 1082–1088. [Google Scholar] [CrossRef] [Green Version]
  37. Dong, X.; Xing, Q.; Li, Y.; Han, X.; Sun, L. Dexmedetomidine protects against ischemia-reperfusion injury in rat skeletal muscle. J. Surg. Res. 2014, 186, 240–245. [Google Scholar] [CrossRef]
  38. Servais, S.; Letexier, D.; Favier, R.; Duchamp, C.; Desplanches, D. Prevention of unloading-induced atrophy by vitamin E supplementation: Links between oxidative stress and soleus muscle proteolysis? Free Radic. Biol. Med. 2007, 42, 627–635. [Google Scholar] [CrossRef] [Green Version]
  39. Bartali, B.; Frongillo, E.A.; Guralnik, J.M.; Stipanuk, M.H.; Allore, H.G.; Cherubini, A.; Bandinelli, S.; Ferrucci, L.; Gill, T.M. Serum micronutrient concentrations and decline in physical function among older persons. JAMA 2008, 299, 308–315. [Google Scholar] [CrossRef] [Green Version]
  40. Mulligan, A.A.; Hayhoe, R.P.G.; Luben, R.N.; Welch, A.A. Positive Associations of Dietary Intake and Plasma Concentrations of Vitamin E with Skeletal Muscle Mass, Heel Bone Ultrasound Attenuation and Fracture Risk in the EPIC-Norfolk Cohort. Antioxidants 2021, 10, 159. [Google Scholar] [CrossRef]
  41. Welch, A.A.; Jennings, A.; Kelaiditi, E.; Skinner, J.; Steves, C.J. Cross-Sectional Associations Between Dietary Antioxidant Vitamins C, E and Carotenoid Intakes and Sarcopenic Indices in Women Aged 18–79 Years. Calcif. Tissue Int. 2020, 106, 331–342. [Google Scholar] [CrossRef] [Green Version]
  42. Overview of Dietary Reference Intakes for Japanese 2015. Available online: http://www.mhlw.go.jp/file/06-Seisakujouhou-10900000-Kenkoukyoku/Overview.pdf (accessed on 25 March 2021).
  43. Li, C.; Kang, B.; Zhang, T.; Gu, H.; Song, P.; Chen, J.; Wang, X.; Xu, B.; Zhao, W.; Zhang, J. Dietary Pattern and Dietary Energy from Fat Associated with Sarcopenia in Community-Dwelling Older Chinese People: A Cross-Sectional Study in Three Regions of China. Nutrients 2020, 12, 3689. [Google Scholar] [CrossRef] [PubMed]
  44. Welch, A.A.; MacGregor, A.J.; Minihane, A.M.; Skinner, J.; Valdes, A.A.; Spector, T.D.; Cassidy, A. Dietary fat and fatty acid profile are associated with indices of skeletal muscle mass in women aged 18-79 years. J. Nutr. 2014, 144, 327–334. [Google Scholar] [CrossRef] [Green Version]
  45. Alhussain, M.H.; Alkahtani, S.; Aljuhani, O.; Habib, S.S. Effects of Nutrient Intake on Diagnostic Measures of Sarcopenia among Arab Men: A Cross-Sectional Study. Nutrients 2020, 13, 114. [Google Scholar] [CrossRef] [PubMed]
  46. Beaudart, C.; Locquet, M.; Touvier, M.; Reginster, J.Y.; Bruyère, O. Association between dietary nutrient intake and sarcopenia in the SarcoPhAge study. Aging Clin. Exp. Res. 2019, 31, 815–824. [Google Scholar] [CrossRef]
  47. Huang, Y.H.; Chiu, W.C.; Hsu, Y.P.; Lo, Y.L.; Wang, Y.H. Effects of Omega-3 Fatty Acids on Muscle Mass, Muscle Strength and Muscle Performance among the Elderly: A Meta-Analysis. Nutrients 2020, 12, 3739. [Google Scholar] [CrossRef]
  48. Corsetto, P.A.; Montorfano, G.; Klersy, C.; Massimino, L.; Infantino, V.; Iannello, G.; Anna Faliva, M.; Lukaski, H.; Perna, S.; Alalwan, T.A.; et al. Fatty Acid Profile and Antioxidant Status Fingerprint in Sarcopenic Elderly Patients: Role of Diet and Exercise. Nutrients 2019, 11, 2569. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  49. Schwingshackl, L.; Hoffmann, G. Monounsaturated fatty acids, olive oil and health status: A systematic review and meta-analysis of cohort studies. Lipids Health Dis. 2014, 13, 154. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  50. Lee, H.; Lim, J.Y.; Choi, S.J. Oleate Prevents Palmitate-Induced Atrophy via Modulation of Mitochondrial ROS Production in Skeletal Myotubes. Oxid. Med. Cell Longev. 2017, 2017, 2739721. [Google Scholar] [CrossRef] [PubMed]
  51. Xue, M.; Zhang, F.; Ji, X.; Yu, H.; Jiang, X.; Qiu, Y.; Yu, J.; Chen, J.; Yang, F.; Bao, Z. Oleate Ameliorates Palmitate-Induced Impairment of Differentiative Capacity in C2C12 Myoblast Cells. Stem. Cells Dev. 2021, 30, 289–300. [Google Scholar] [CrossRef] [PubMed]
  52. Højfeldt, G.; Nishimura, Y.; Mertz, K.; Schacht, S.R.; Lindberg, J.; Jensen, M.; Hjulmand, M.; Lind, M.V.; Jensen, T.; Jespersen, A.P.; et al. Daily Protein and Energy Intake Are Not Associated with Muscle Mass and Physical Function in Healthy Older Individuals-A Cross-Sectional Study. Nutrients 2020, 12, 2794. [Google Scholar] [CrossRef]
  53. Bollwein, J.; Diekmann, R.; Kaiser, M.J.; Bauer, J.M.; Uter, W.; Sieber, C.C.; Volkert, D. Distribution but not amount of protein intake is associated with frailty: A cross-sectional investigation in the region of Nürnberg. Nutr. J. 2013, 12, 109. [Google Scholar] [CrossRef] [Green Version]
  54. Das, A.; Cumming, R.G.; Naganathan, V.; Blyth, F.; Le Couteur, D.G.; Handelsman, D.J.; Waite, L.M.; Ribeiro, R.V.; Simpson, S.J.; Hirani, V. Associations between nutrient intakes and dietary patterns with different sarcopenia definitions in older Australian men: The concord health and ageing in men project. Public Health Nutr. 2020, 10, 1–16. [Google Scholar] [CrossRef]
  55. Ministry of Health, Labour and Welfare. The Report of National Health and Nutrition Survey (2018). Available online: https://www.mhlw.go.jp/stf/seisakunitsuite/bunya/kenkou_iryou/kenkou/eiyou/h30-houkoku_00001.html (accessed on 25 March 2021).
Nutrients 13 01730 g001 550
Figure 1. Flow chart of the recruitment of participants in this study.
Figure 1. Flow chart of the recruitment of participants in this study.
Nutrients 13 01730 g001
Table
Table 1. Baseline characteristics of participants.
Table 1. Baseline characteristics of participants.
CharacteristicOverall
(n = 1345)		Men
(n = 437)		Women
(n = 908)		p Value *
Age (y)71.2 (7.4)71.4 (7.9)71.1 (7.1)0.513
Height (cm)156.1 (8.9)165.3 (6.3)151.7 (6.1)<0.001
Weight (cm)55.6 (10.9)63.8 (10.8)51.7 (8.5)<0.001
BMI (kg/m2)22.7 (3.4)23.3 (3.4)22.5 (3.4)<0.001
Residing in a coastal area (%)54.953.155.80.343
Current smoking habit (%)7.016.52.4<0.001
Current alcohol drinking habit (%)40.566.628.0<0.001
Usual walking speed (m/s)1.17 (0.26)1.16 (0.25)1.18 (0.26)0.140
Handgrip strength (kg)29.1 (9.5)39.6 (7.8)24.0 (4.8)<0.001
Appendicular muscle mass index (kg/m2)6.6 (1.1)7.7 (1.1)6.0 (0.6)<0.001
Prevalence of sarcopenia (%)5.75.06.10.450
Values are means (standard deviation). BMI, body mass index. * Continuous variables, Student’s t-test; categorical variables, chi-squared test, and p < 0.05 shown as boldface.
Table
Table 2. Dietary intakes of total energy and 50 major nutrient factors in participants with and without sarcopenia.
Table 2. Dietary intakes of total energy and 50 major nutrient factors in participants with and without sarcopenia.
CategoryNutrient FactorSarcopenia (+)
(n = 77)		Sarcopenia (−)
(n = 1268)		p Value *
CalorieTotal energy (kcal/day)1808.4 (522.2)1877.8 (559.4)0.289
Major nutrientProtein (g/day)73.3 (27.4)75.9 (28.2)0.433
Animal protein (g/day)44.8 (21.8)46.6 (22.4)0.493
Vegetable protein (g/day)28.5 (8.6)29.3 (9.2)0.462
Fat (g/day)50.6 (19.2)55.7 (20.6)0.032
Animal fat (g/day)25.4 (12.1)27.3 (12.2)0.181
Vegetable fat (g/day)25.2 (10.2)28.4 (11.1)0.013
Carbohydrate (g/day)249.6 (72.0)247.5 (79.3)0.823
MineralAsh (g/day)18.8 (6.4)19.6 (6.5)0.268
Sodium (mg/day)4314.7 (1385.3)4483.4 (1506.3)0.338
Potassium (mg/day)2557.4 (1039.6)2709.8 (1016.6)0.202
Calcium (mg/day)604.8 (279.3)613.1 (261.3)0.788
Magnesium (mg/day)253.7 (94.4)268.8 (94.7)0.175
Phosphorus (mg/day)1124.6 (434.7)1163.6 (436.6)0.447
Iron (mg/day)7.8 (3.1)8.1 (3.1)0.382
Zinc (mg/day)8.1 (2.7)8.4 (2.8)0.400
Copper (mg/day)1.1 (0.4)1.2 (0.4)0.717
Manganese (mg/day)3.2 (1.1)3.2 (1.1)0.825
Fat-soluble vitaminα-Carotene (μg/day)465.4 (440.0)421.1 (328.5)0.261
β-Carotene (μg/day)3165.3 (2648.5)3098.7 (2036.6)0.784
Retinol (μg/day)474.5 (502.9)444.1 (404.2)0.527
Cryptoxanthin (μg/day)448.7 (400.0)505.7 (402.8)0.228
β-Carotene equivalents (μg/day)3627.0 (2919.5)3567 (2246.2)0.823
Retinol equivalents (μg/day)780.5 (587.9)745.2 (485.7)0.542
Vitamin D (μg/day)21.3 (14.6)20.8 (14.3)0.770
α-Tocopherol (mg/day)7.2 (3.1)7.9 (3.1)0.049
β-Tocopherol (mg/day)0.3 (0.1)0.4 (0.1)0.004
γ-Tocopherol (mg/day)11.0 (4.7)12.7 (5.1)0.003
δ-Tocopherol (mg/day)2.8 (1.1)3.1 (1.2)0.033
Vitamin K (μg/day)256.1 (165.1)269.5 (165.2)0.489
Water-soluble
vitamin		Vitamin B1 (mg/day)0.8 (0.3)0.8 (0.3)0.143
Vitamin B2 (mg/day)1.3 (0.5)1.4 (0.5)0.438
Niacin (mg/day)17.6 (7.6)19.2 (7.7)0.077
Vitamin B6 (mg/day)1.3 (0.5)1.4 (0.5)0.308
Vitamin B12 (μg/day)12.6 (7.8)12.8 (7.8)0.844
Folic acid (μg/day)330.3 (154.9)348.5 (145.6)0.287
Pantothenic acid (mg/day)6.6 (2.4)6.8 (2.4)0.573
Vitamin C (mg/day)125.6 (65.7)135.2 (69.2)0.235
Fatty acidSaturated fatty acid (g/day)13.6 (5.5)14.7 (5.8)0.105
Monounsaturated fatty acid (g/day)17.6 (7.2)19.8 (7.6)0.016
Polyunsaturated fatty acid (g/day)12.0 (4.5)13.4 (5.1)0.014
n-3 fatty acid (g/day)2.9 (1.4)3.1 (1.5)0.333
n-6 fatty acid (g/day)9.0 (3.3)10.3 (3.8)0.004
OthersCholesterol (mg/day)420.0 (199.7)436.7 (200.6)0.479
Soluble dietary fiber (g/day)2.9 (1.3)3.1 (1.3)0.194
Insoluble dietary fiber (g/day)8.5 (3.4)8.9 (3.4)0.241
Dietary fiber (g/day)11.8 (4.9)12.4 (4.9)0.319
Salt equivalent (g/day)10.9 (3.5)11.3 (3.8)0.341
Sucrose (g/day)13.9 (9.2)14.2 (9.8)0.845
Alcohol (g/day)5.4 (14.9)8.7 (18.9)0.127
Daidzein (mg/day)12.5 (9.0)12.4 (9.3)0.987
Genistein (mg/day)21.2 (15.3)21.2 (15.8)0.986
Values are means (standard deviation). * Student’s t-test, and p < 0.05 shown as boldface.
Table
Table 3. Prevalence of sarcopenia according to the quantile of the intakes of vitamin E and fats.
Table 3. Prevalence of sarcopenia according to the quantile of the intakes of vitamin E and fats.
Nutrient FactorCharacteristicDaily IntakeTrend p *
Q1 (Low)Q2Q3Q4 (High)
FatMedian (range) of intake (g/day) †33.8 (6.8–41.4)47.5 (41.5–53.4)59.2 (53.5–66.7)77.9 (66.7–143.8)
N with or without sarcopenia ‡23/31321/31622/31411/325
Adjusted OR (95% CI) §1.00 (reference)1.01 (0.45–2.27)0.75 (0.31–1.82)0.27 (0.08–0.96)0.037
Vegetable fatMedian (range) of intake (g/day) †16.5 (1.9–20.5)24.1 (20.5–27.1)30.2 (27.1–34.6)40.0 (34.6–75.8)
N with or without sarcopenia ‡27/30920/31717/31913/323
Adjusted OR (95% CI) §1.00 (reference)0.86 (0.40–1.83)0.67 (0.28–1.58)0.53 (0.18–1.50)0.202
α-TocopherolMedian (range) intake (mg/day) †4.6 (0.9–5.7)6.6 (5.7–7.5)8.4 (7.5–9.6)11.2 (9.6–21.8)
N with or without sarcopenia ‡25/31117/32026/3109/327
Adjusted OR (95% CI) §1.00 (reference)0.68 (0.30–1.55)0.92 (0.38–2.19)0.14 (0.04–0.49)0.008
β-TocopherolMedian (range) intake (mg/day) †0.21 (0.02–0.27)0.31 (0.27–0.35)0.39 (0.35–0.44)0.51 (0.44–0.95)
N with or without sarcopenia ‡24/31224/31222/3157/329
Adjusted OR (95% CI) §1.00 (reference)0.93 (0.44–1.94)0.78 (0.34–1.78)0.24 (0.07–0.78)0.018
γ-TocopherolMedian (range) intake (mg/day) †7.2 (0.9–9.2)10.6 (9.2–12.0)13.7 (12.0–15.4)18.2 (15.4–36.1)
N with or without sarcopenia ‡25/31122/31423/3147/329
Adjusted OR (95% CI) §1.00 (reference)1.31 (0.62–2.77)1.07 (0.49–2.38)0.28 (0.09–0.87)0.024
δ-TocopherolMedian (range) intake (mg/day) †1.7 (0.3–2.2)2.6 (2.2–2.9)3.3 (2.9–3.8)4.4 (3.8–8.5)
N with or without sarcopenia ‡22/31424/31320/31611/325
Adjusted OR (95% CI) §1.00 (reference)1.60 (0.75–3.42)1.09 (0.47–2.55)0.75 (0.26–2.12)0.409
Monounsaturated fatty acidMedian (range) intake (g/day) †11.8 (2.0–14.5)16.5 (14.6–18.8)20.9 (18.8–24.0)28.0 (24.0–53.3)
N with or without sarcopenia ‡25/31122/31519/31711/325
Adjusted OR (95% CI) §1.00 (reference)0.94 (0.43–2.05)0.69 (0.29–1.67)0.22 (0.07–0.72)0.011
Polyunsaturated fatty acidMedian (range) intake (g/day) †8.0 (1.6–9.9)11.4 (10.0–12.7)14.2 (12.7–16.0)18.9 (16.0–34.1)
N with or without sarcopenia ‡23/31322/31422/31510/326
Adjusted OR (95% CI) §1.00 (reference)1.19 (0.54–2.63)0.96 (0.41–2.23)0.28 (0.09–0.89)0.029
n-6 fatty acidMedian (range) intake (g/day) †6.2 (0.9–7.6)8.7 (7.6–9.8)10.9 (9.8–12.2)14.3 (12.2–26.3)
N with or without sarcopenia ‡23/31326/31020/3178/328
Adjusted OR (95% CI) §1.00 (reference)1.65 (0.77–3.51)1.01 (0.42–2.39)0.39 (0.12–1.26)0.064
OR: odds ratio, CI: confidence interval, Q: quantile. * Based on logistic regression analysis according to the quantile categories of nutrient intakes. p for trend estimated by treating the quantiles as ordinal variables for nutrient intakes. † Values at baseline. ‡ The number of subjects with or without sarcopenia. § Adjusted for age, sex, BMI, residence area, current smoking habit, current alcohol drinking habit, and total caloric intake. p < 0.05 shown as boldface.
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Share and Cite

MDPI and ACS Style

Otsuka, Y.; Iidaka, T.; Horii, C.; Muraki, S.; Oka, H.; Nakamura, K.; Izumo, T.; Rogi, T.; Shibata, H.; Tanaka, S.; et al. Dietary Intake of Vitamin E and Fats Associated with Sarcopenia in Community-Dwelling Older Japanese People: A Cross-Sectional Study from the Fifth Survey of the ROAD Study. Nutrients 2021, 13, 1730. https://0-doi-org.brum.beds.ac.uk/10.3390/nu13051730

AMA Style

Otsuka Y, Iidaka T, Horii C, Muraki S, Oka H, Nakamura K, Izumo T, Rogi T, Shibata H, Tanaka S, et al. Dietary Intake of Vitamin E and Fats Associated with Sarcopenia in Community-Dwelling Older Japanese People: A Cross-Sectional Study from the Fifth Survey of the ROAD Study. Nutrients. 2021; 13(5):1730. https://0-doi-org.brum.beds.ac.uk/10.3390/nu13051730

Chicago/Turabian Style

Otsuka, Yuta, Toshiko Iidaka, Chiaki Horii, Shigeyuki Muraki, Hiroyuki Oka, Kozo Nakamura, Takayuki Izumo, Tomohiro Rogi, Hiroshi Shibata, Sakae Tanaka, and et al. 2021. "Dietary Intake of Vitamin E and Fats Associated with Sarcopenia in Community-Dwelling Older Japanese People: A Cross-Sectional Study from the Fifth Survey of the ROAD Study" Nutrients 13, no. 5: 1730. https://0-doi-org.brum.beds.ac.uk/10.3390/nu13051730

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop