Sulfated polysaccharide-rich extract from <i>Navicula incerta</i>: physicochemical characteristics, antioxidant activity, and anti-hemolytic property

Ricardo I. González-Vega; Carmen L. Del-Toro-Sánchez; Ramón A. Moreno-Corral; José A. López-Elías; Aline Reyes-Díaz; Norma García-Lagunas; Elizabeth Carvajal-Millán; Diana Fimbres-Olivarría; Ricardo I. González-Vega; Carmen L. Del-Toro-Sánchez; Ramón A. Moreno-Corral; José A. López-Elías; Aline Reyes-Díaz; Norma García-Lagunas; Elizabeth Carvajal-Millán; Diana Fimbres-Olivarría

doi:10.3934/bioeng.2022027

AIMS Bioengineering

2022, Volume 9, Issue 4: 364-382. doi: 10.3934/bioeng.2022027

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Sulfated polysaccharide-rich extract from Navicula incerta: physicochemical characteristics, antioxidant activity, and anti-hemolytic property

1.
University of Guadalajara (UDG), Cienega University Center, Universidad Ave. 1115, Lindavista, 47820, Ocotlán, Jalisco, México
2.
University of Sonora (UNISON), Rosales y Niños Héroes S/N, 83000, Hermosillo, Sonora, México
3.
State University of Sonora (UES), Navojoa Academic Unit, Manlio Fabio Beltrones Blvd. 810, 85875, Navojoa, Sonora, México
4.
Research Center for Food and Development (CIAD, A.C.), Carretera Gustavo Enrique Astiazarán Rosas, 46, 83304, Hermosillo, Sonora, México

Received: 09 October 2022 Revised: 23 November 2022 Accepted: 06 December 2022 Published: 23 December 2022

A sulfated polysaccharide from Navicula incerta (SPNi) was extracted, and its physicochemical characteristics, antioxidant activity, and anti-hemolytic property were investigated. The polysaccharide yield was 4.8% (SPNi weight/biomass dry weight). Glucose, galactose, mannose, and xylose were the primary sugars. The sulfate content and Mw values were 0.46% and 45 kDa, respectively. The FT-IR spectrum showed characteristic bands at 3276,1079,1255, and 820 cm⁻¹, related to -OH, C-O-C, S=O, and C-O-S stretching vibration. The ¹H-NMR analysis revealed signals of anomeric protons, indicating the presence of CH₂-O and CH-O groups. SPNi registered ferric-reducing antioxidant power (up to 1.47 µmol TE/g) and 54% anti-radical activity on ABTS⁺. This polysaccharide registered 90% hemolysis inhibition achieving integrity of the erythrocyte membrane. The results indicate that SPNi could be a candidate for biotechnology applications where antioxidant activity and hemolysis inhibition are required.
- Navicula incerta,
- benthic diatoms,
- sulfated polysaccharide,
- antioxidant activity,
- anti-hemolytic property,
- bioactivity
Citation: Ricardo I. González-Vega, Carmen L. Del-Toro-Sánchez, Ramón A. Moreno-Corral, José A. López-Elías, Aline Reyes-Díaz, Norma García-Lagunas, Elizabeth Carvajal-Millán, Diana Fimbres-Olivarría. Sulfated polysaccharide-rich extract from Navicula incerta: physicochemical characteristics, antioxidant activity, and anti-hemolytic property[J]. AIMS Bioengineering, 2022, 9(4): 364-382. doi: 10.3934/bioeng.2022027

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Abstract

A sulfated polysaccharide from Navicula incerta (SPNi) was extracted, and its physicochemical characteristics, antioxidant activity, and anti-hemolytic property were investigated. The polysaccharide yield was 4.8% (SPNi weight/biomass dry weight). Glucose, galactose, mannose, and xylose were the primary sugars. The sulfate content and Mw values were 0.46% and 45 kDa, respectively. The FT-IR spectrum showed characteristic bands at 3276,1079,1255, and 820 cm⁻¹, related to -OH, C-O-C, S=O, and C-O-S stretching vibration. The ¹H-NMR analysis revealed signals of anomeric protons, indicating the presence of CH₂-O and CH-O groups. SPNi registered ferric-reducing antioxidant power (up to 1.47 µmol TE/g) and 54% anti-radical activity on ABTS⁺. This polysaccharide registered 90% hemolysis inhibition achieving integrity of the erythrocyte membrane. The results indicate that SPNi could be a candidate for biotechnology applications where antioxidant activity and hemolysis inhibition are required.

Acknowledgments

This research received funding from CONACYT, grant number 319684. The authors are pleased to acknowledge the DICTUS, CIAD, DIPA, and DIPM spectroscopy laboratory of Universidad de Sonora for use of the facilities to characterize the material.

Conflict of interest

All authors declare no conflicts of interest in this paper.

Author contributions

Ricardo I. González-Vega: Conceptualization, methodology, formal analysis, investigation, writing—review and editing, visualization, supervision. Carmen L. Del-Toro-Sánchez: resources, writing—review and editing. Ramón A. Moreno-Corral: methodology, writing—review and editing. José A. López-Elías: resources, writing—review and editing. Aline Reyes-Díaz: methodology, writing—review and editing. Norma García-Lagunas: formal analysis, writing—review and editing. Elizabeth Carvajal-Millán: Supervision, Project administration, Funding acquisition, Conceptualization, Writing- Reviewing and Editing, Validation. Diana Fimbres-Olivarría: Conceptualization, methodology, investigation, writing—original draft preparation, writing—review and editing, visualization, supervision.

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