Paper Titles

Octacalcium Phosphate: A Potential Scaffold Material for Controlling Activity of Bone-Related Cells In Vitro
p.1366

Effect of Phase Stability on Some Physical and Mechanical Properties in β-Ti Single Crystal for Biomedical Applications
p.1372

Biocompatibility of Titanium Dioxide Film Modified by Femtosecond Laser Irradiation
p.1377

Chemical-Hydrothermal Synthesis of Zr-Containing Titanium Oxide Film on CP-Ti
p.1383

Substrate Independent Approach for Immobilisation of Quaternary Ammonium Compounds to Surfaces to Reduce Bio-Burden
p.1389

Plasma Modification of DLC Films and the Resulting Surface Biocompatibility
p.1396

Comparison of Various Electroless Nickel Coatings on Steel: Structure, Hardness and Abrasion Resistance
p.1405

Formation of Fe-Al Intermetallic Compound Film on High-Speed Tool Steel by Shot Lining and Heat Treatment
p.1414

Influence of SiO₂ Nanoparticle Codeposition on Homogeneity of Zinc-Nickel Alloy Plating from an Acid Sulphate Bath
p.1420

HomeMaterials Science ForumMaterials Science Forum Vols. 783-786Substrate Independent Approach for Immobilisation...

Substrate Independent Approach for Immobilisation of Quaternary Ammonium Compounds to Surfaces to Reduce Bio-Burden

Article Preview

Abstract:

Bacterial contamination of biomedical devices is an ongoing problem. One method to alleviate such contamination is the introduction of surface compounds onto devices which can kill bacteria on contact. Polymers containing quaternary ammonium groups are known for their antimicrobial properties. Here we report a substrate-independent two-step method for the immobilisation of quaternary ammonium groups onto any type of surface. To achieve this glycidlytrimethylammonium chloride was covalently bound to plasma polymerised allylmine interlayer. Changes in the membrane permeability of Escherichia coli were observed by BacLight LIVE\DEAD staining. 30% of E. coli grown on the treated surfaces showed high levels of membrane permeability within 4 hours. Importantly, there was no observable cytotoxic effect on human dermal fibroblasts.

You might also be interested in these eBooks

THERMEC 2013

Info:

Periodical:

Materials Science Forum (Volumes 783-786)

Pages:

1389-1395

DOI:

https://doi.org/10.4028/www.scientific.net/MSF.783-786.1389

Citation:

Cite this paper

Online since:

May 2014

Authors:

Alex Cavallaro*, Peter Majewski, Mary Barton, Krasimir Vasilev

Keywords:

Antibacterial Coatings, Infections, Medical Devices, Plasma Polymerisation, Quaternary Ammonium Compounds, Surface Modification

Export:

RIS, BibTeX

Price:

Permissions:

Request Permissions

* - Corresponding Author

[1] World Health Organization. Annex Table 2: Deaths by cause, sex and mortality stratum in WHO regions, estimates for 2002. The world health report. (2004).

[2] McCann MT, Gilmore BF, Gorman SP. Staphylococcus epidermidis device-related infections: pathogenesis and clinical management. Journal of Pharmacy and Pharmacology. 2008; 60: 1551-71.

DOI: 10.1211/jpp.60.12.0001

[3] Fux CA, Costerton JW, Stewart PS, Stoodley P. Survival strategies of infectious biofilms. Trends in Microbiology. 2005; 13: 34-40.

DOI: 10.1016/j.tim.2004.11.010

[4] Vasilev K, Cook J, Griesser HJ. Antibacterial surfaces for biomedical devices. Expert Rev Med Devices. 2009; 6: 553-67.

DOI: 10.1586/erd.09.36

[5] Vasilev K, Griesser SS, Griesser HJ. Antibacterial Surfaces and Coatings Produced by Plasma Techniques. Plasma Processes and Polymers. 2011; 8: 1010-23.

DOI: 10.1002/ppap.201100097

[6] Tiller JC, Liao C-J, Lewis K, Klibanov AM. Designing surfaces that kill bacteria on contact. Proceedings of the National Academy of Sciences. 2001; 98: 5981-5.

DOI: 10.1073/pnas.111143098

[7] Gottenbos B, van der Mei HC, Klatter F, Nieuwenhuis P, Busscher HJ. In vitro and in vivo antimicrobial activity of covalently coupled quaternary ammonium silane coatings on silicone rubber. Biomaterials. 2002; 23: 1417-23.

DOI: 10.1016/s0142-9612(01)00263-0

[8] Isquith AJ, Abbott EA, Walters PA. Surface-bonded antimicrobial activity of an organosilicon quaternary ammonium chloride. Appl Microbiol. 1972; 24: 859-63.

DOI: 10.1128/am.24.6.859-863.1972

[9] Vasilev K, Michelmore A, Griesser HJ, Short RD. Substrate Influence on the Initial Growth Phase of Plasma-Deposited Polymer Films. Chemical Communications. 2009: 3600 - 2.

DOI: 10.1039/b904367e

[10] Vasilev K, Michelmore A, Martinek P, Chan J, Sah V, Griesser HJ, et al. Early Stages of Growth of Plasma Polymer Coatings Deposited from Nitrogen- and Oxygen-Containing Monomers. Plasma Processes and Polymers. 2010; 7: 824-35.

DOI: 10.1002/ppap.201000030

[11] Weinstein RA, Gaynes R, Edwards JR, System NNIS. Overview of Nosocomial Infections Caused by Gram-Negative Bacilli. Clinical Infectious Diseases. 2005; 41: 848-54.

DOI: 10.1086/432803

[12] Vasilev K, Mierczynska A, Hook AL, Chan J, Voelcker NH, Short RD. Creating gradients of two proteins by differential passive adsorption onto a PEG-density gradient. Biomaterials. 2010; 31: 392-7.

DOI: 10.1016/j.biomaterials.2009.09.056

[13] Goreham RV, Short RD, Vasilev K. Method for the Generation of Surface-Bound Nanoparticle Density Gradients. Journal of Physical Chemistry C. 2011; 115: 3429-33.

DOI: 10.1021/jp111221g

[14] Kistamah N, Carr CM, Rosunee S. X-ray photoelectron spectroscopic study of Tencel treated with a cationic β-cyclodextrin derivative. Surface and Interface Analysis. 2009; 41: 710-3.

DOI: 10.1002/sia.3076

[15] Mierczynska A, Michelmore A, Tripathi A, Goreham RV, Sedev R, Vasilev K. pH-tunable gradients of wettability and surface potential. Soft Matter. 2012; 8: 8399-404.

DOI: 10.1039/c2sm25221j

[16] Ferreira C, Pereira AM, Pereira MC, Melo LF, Simões M. Physiological changes induced by the quaternary ammonium compound benzyldimethyldodecylammonium chloride on Pseudomonas fluorescens. Journal of Antimicrobial Chemotherapy. (2011).

DOI: 10.1093/jac/dkr028

[17] Kügler R, Bouloussa O, Rondelez F. Evidence of a charge-density threshold for optimum efficiency of biocidal cationic surfaces. Microbiology. 2005; 151: 1341-8.

DOI: 10.1099/mic.0.27526-0

[18] Murata H, Koepsel RR, Matyjaszewski K, Russell AJ. Permanent, non-leaching antibacterial surfaces-2: How high density cationic surfaces kill bacterial cells. Biomaterials. 2007; 28: 4870-9.

DOI: 10.1016/j.biomaterials.2007.06.012

[19] Terada A, Yuasa A, Kushimoto T, Tsuneda S, Katakai A, Tamada M. Bacterial adhesion to and viability on positively charged polymer surfaces. Microbiology. 2006; 152: 3575-83.

DOI: 10.1099/mic.0.28881-0

[20] Ioannou CJ, Hanlon GW, Denyer SP. Action of disinfectant quaternary ammonium compounds against Staphylococcus aureus. Antimicrob Agents Chemother. 2007; 51: 296-306.

DOI: 10.1128/aac.00375-06

[21] Delcour AH. Outer membrane permeability and antibiotic resistance. Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics. 2009; 1794: 808-16.

DOI: 10.1016/j.bbapap.2008.11.005

[22] Hancock REW. The bacterial outer membrane as a drug barrier. Trends in Microbiology. 1997; 5: 37-42.

[23] Abdull Rasad MSB, Halim AS, Hashim K, Rashid AHA, Yusof N, Shamsuddin S. In vitro evaluation of novel chitosan derivatives sheet and paste cytocompatibility on human dermal fibroblasts. Carbohydrate Polymers. 2010; 79: 1094-100.

DOI: 10.1016/j.carbpol.2009.10.048

[24] Werner S, Krieg T, Smola H. Keratinocyte-Fibroblast Interactions in Wound Healing. Journal of Investigative Dermatology. 2007; 127: 998-1008.

DOI: 10.1038/sj.jid.5700786