After the immunomagnetic beads prepared separately for the capture of Yersinia enterocolitica 03, 05, 27, 08 and 09 were mixed at an equal ratio, the mixemagnetic bead suspension was used for the simultaneous detection of these serovars by IMS-CINplating followed by PCR. The proposed method was shown to be capable of detecting specificallyand simultaneously 03, 05, 27, 08 and 09 from food and fecal samples within 2 days if anyof these bacteria were present at more than 10⁰ cfu/ml in the sample suspension.