EpiSCORE: Cell-type deconvolution of bulk tissue DNA methylomes from single-cell RNA-Seq data
Description
EpiSCORE is an R-package for constructing a tissue-specific DNA methylation reference matrix that can be subsequently used in conjunction with a reference-based cell-type deconvolution algorithm to (i) obtain cell-type fraction estimates in a corresponding bulk-tissue sample for which a genome-wide DNAm profile exists, and (ii) to infer cell-type specific differential DNA methylation signals in the context of a general Epigenome-Wide-Association Study. EpiSCORE is aimed particularly at complex solid tissues, for which experimentally generating an appropriate DNAm reference matrix representing all the major cell-types within the tissue is not possible. EpiSCORE exploits the tissue-specific single-cell RNA-Sequencing atlases to construct corresponding tissue-specific DNA methylation references.
Files
Files
(92.5 MB)
Name | Size | Download all |
---|---|---|
md5:613cca148beed5bdc06d14692f074690
|
42.0 MB | Download |
md5:545a765bc6580d60d2a8a2aa9ee35832
|
50.5 MB | Download |