Enhanced genome annotation strategy provides novel insights on the phylogeny of 'Flaviviridae': Supplementary material

SUPPLEMENTARY MATERIAL

Index

• Table S1 (tableS1.csv): genomic data.

• Table S2 (tableS2.csv): character categorization for selected nodes.

• Table S3 (tableS3.csv): programs and parameters.

• Table S4 (tableS4.csv): annotation efficiency.

• File S1 (fileS1.gff): gene annotation.

• File S2 (fileS2.xml): configuration file for BEAST 2 (configuration.xml).

• Figure S1 (figureS1.pdf): dendrogram depicting the hierarchical clusters of trees based on match-split distances.

• Figure S2 (figureS2.pdf): full version of the working phylogenetic hypothesis (tree No. 0 in table 1).

Figure captions

• Figure S1: A dendrogram depicting the hierarchical clusters of trees based on match-split distances. Outgroup sequences (Hepacivirus, Pegivirus, and Pestivirus) were removed to guarantee the compared tree topologies would have the same terminals. Tree numbers correspond to those in table 1 of the manuscript. I. No outgroup sequences; some matrices were partitioned. II. Outgroup sequences and partitioned matrices. *This tree was produced without outgroup sequences.
• Figure S2: Full version of the working phylogenetic hypothesis (tree No. 0 in table 1). Branch lengths represent an estimation of the number of substitutions per site. Node labels indicate SH-aLRT support / ultrafast bootstrap (only shown if one of there is a value below 90%). Clade names correlate to the character categorization analysis (see table S2). Branch labels represent the four genera: I = Pestivirus; II = Pegivirus; III = Hepacivirus; IV = Flavivirus. * The Ecuador Paraiso Escondido virus (EPEV) was isolated from sand flies (Psathyromyia abonnenci). The EPEV was the first sand fly-borne Flavivirus identified in the New World.

Manuscript title

FLAVi: an enhanced annotator for viral genomes of Flaviviridae.

Authors

• de Bernardi Schneider, Adriano. University of California San Diego. ORCID: 0000-0001-7487-266X.

• Jacob Machado, Denis. University of North Carolina at Charlotte. ORCID: 0000-0001-9858-4515. Corresponding author.

• Guirales, Sayal. University of North Carolina at Charlotte.

• Janies, Daniel. University of North Carolina at Charlotte.

First author: Adriano de Bernardi Schneider and Denis Jacob Machado have contributed equally to the manuscript.

Contact information

• Corresponding author: Denis Jacob Machado, Ph.D.

• OrcID: 0000-0001-9858-4515.

• Email: dmachado [at] uncc.edu.

Other additional material

• In addition to the material listed above, all 31 tree topologies and 15 alignment matrices discussed in this manuscript will are available in TreeBASE (http://purl.org/phylo/treebase/phylows/study/TB2:S24096) after the publication of the manuscript.
• The FLAVi pipeline and all the original scripts are available at GitLab (https://gitlab.com/MachadoDJ/FLAVi).
• The web application can be accessed at http://flavi-web.com.