Script documents for genome assembly of the maize inbred line A188
Creators
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Guifang Lin
-
Cheng He
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Jun Zheng
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Dal-Hoe Koo
- Ha Le1
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Huakun Zheng
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Tej Man Tamang
- Jinguang Lin1
- Yan Liu2
- Mingxia Zhao1
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Yangfan Hao
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Frank McFarland
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Bo Wang
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Yang Qin
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Haibao Tang
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Donald R McCarty
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Hairong Wei
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Myeong-Je Cho
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Sunghun Park
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Heidi Kaeppler
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Shawn M Kaeppler
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Yunjun Liu
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Nathan Springer
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Patrick S Schnable
- Guoying Wang2
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Frank White
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Sanzhen Liu
- 1. Kansas State University
- 2. Chinese Academy of Agricultural Sciences
Description
The inbred line A188 is an attractive model for elucidation of maize gene function and improvement due to the high embryogenic and transformable character of the line. A188 exhibits many contrasting traits to the inbred line B73, the first maize reference genome, and other elite lines. The lack of a genome sequence of A188 limits the use of A188 as a model for functional studies. We have assembled the A188 genome with Nanopore long reads and Bionano optical maps. The A188 genome assembly provides a high-resolution sequence for a complex genome species and a foundational resource for analyses of genome variation and gene function in maize. The genome, in comparison to B73, contains extensive intra-species structural variations and other genetic differences. Expression and network analyses identified discrete profiles for embryonic callus and other tissues. Here, we deposited the scripts used for the genome assembly of A188.
Files
A188Ref1.zip
Files
(320.4 MB)
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Additional details
Funding
- ECA-PGR: Under the Hood: The Genetic Components of Maize Transformation 1741090
- National Science Foundation