Rapid increase in transferrin receptor recycling promotes adhesion during T cell activation

This Dataset contains primary data used for the publication "Rapid increase in transferrin receptor recycling promotes adhesion during T cell activation" accepted for publication in BMC Biology on 15. July 2022

Abstract:

Background
T cell activation leads to increased expression of the receptor for the iron transporter transferrin (TfR) to provide iron required for the cell differentiation and clonal expansion that takes place during the days after encounter with a cognate antigen. However, T cells mobilise TfR to their surface within minutes after activation, although the reason and mechanism driving this process remain unclear.

Results
Here we show that T cells transiently increase endocytic uptake and recycling of TfR upon activation, thereby boosting their capacity to import iron. We demonstrate that increased TfR recycling is powered by a fast endocytic sorting pathway relying on the membrane proteins flotillins, Rab5 and Rab11a-positive endosomes. Our data further reveal that iron import is required for a non-canonical signalling pathway involving the kinases Zap70 and PAK, which controls adhesion of the integrin LFA-1 and eventually leads to conjugation with antigen-presenting cells.

Conclusions
Altogether, our data suggest that T cells boost their iron importing capacity immediately upon activation to promote adhesion to antigen-presenting cells.

Data are organised in compressed (.zip) folders entitled as the corresponding Figures in the publication.

Programs we recommend to view the files are:
.fcs files:    FlowJo software v10 (Tree Star, Ashland, OR, USA)
.lsm and .czi files: ZEN 2012 SP1 and higher (Carl Zeiss, Jena, Germany)
.lif files:       LAS X v3 (Leica Microsystems, Wetzlar, Germany)
.pzfx files:   Prism v7 software (GraphPad, San Diego, CA, USA)