Comparative Screening Methods for the Detection of Biosurfactant-Producing Capability of Antarctic Hydrocarbon-degrading Pseudomonas sp.

Authors

  • Nurul Hani Saruni Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
  • Syafiqah Abdul Razak Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, D.E, Malaysia.
  • Syahir Habib Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, D.E, Malaysia.
  • Siti Aqlima Ahmad Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, D.E, Malaysia.
  • Siti Aisyah Alias National Antarctic Research Centre, B303 Level 3, Block B, IPS Building, Universiti Malaya, 50603,Kuala Lumpur, Malaysia.
  • Wan Lutfi Wan Johari Department of Environmental Sciences, Faculty of Environmental Studies, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
  • Jerzy Smykla Institute of Nature Conservation, Polish Academy of Sciences, Mickiewicza, 33, 31-120, Kraków, Poland.
  • Nur Adeela Yasid Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, D.E, Malaysia.

DOI:

https://doi.org/10.54987/jemat.v7i1.471

Keywords:

Biosurfactants; Pseudomonas sp.; hydrocarbon-degrading strain; preliminary screening

Abstract

Four preliminary screening methods for biosurfactant synthesis - drop collapse assay, oil displacement activity, microplate assay and emulsification index (E24) were compared and evaluated for their reliability and ease of use. All screening methods showed positive indications for the synthesis of biological surface-active agents. Nevertheless, partial collapse of the supernatant and low emulsification index (E24) of Pseudomonas sp. might signify a low production of biosurfactants. Based on our observation, both drop collapse and oil displacement assay is the fastest, easiest and most reliable analytical routine to be suggested to screen for biosurfactant-producing strains. In the extent for a high throughput screening (HTS), drop collapse assay is the best method for an accurate screening of biosurfactant producers.

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Published

31.07.2019

How to Cite

Saruni, N. H., Abdul Razak, S., Habib, S., Ahmad, S. A., Alias, S. A., Wan Johari, W. L., Smykla, J., & Yasid, N. A. (2019). Comparative Screening Methods for the Detection of Biosurfactant-Producing Capability of Antarctic Hydrocarbon-degrading Pseudomonas sp. Journal of Environmental Microbiology and Toxicology, 7(1), 44–47. https://doi.org/10.54987/jemat.v7i1.471

Issue

Vol. 7 No. 1 (2019)

Section

Articles

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