Biomed. Papers 145(2), 81-83 (2001) | DOI: 10.5507/bp.2001.019

DETERMINATION OF TOTAL ANTIOXIDANT CAPACITY IN PLASMA BY CYCLIC VOLTAMMETRY. TWO CASE REPORTS

Jitka Psotová^a, Jana Zahálková^b, Jan Hrbáč^c, Vilím Šimánek^a, Josef Bartek^a

^a Institute of Medical Chemistry and Biochemistry, Palacký University, 775 15 Olomouc, psotova@tunw.upol.cz

^b 3rd Clinic of Internal Medicine, University Hospital Olomouc

^c Institute of Inorganic and Physical Chemistry, Faculty of Science, Palacký University, Olomouc, Czech Republic

The cyclic voltammetry (CV) was used for the measurement of the plasma total antioxidant capacity from twotypes of patients. The first one consisted of 29 volunteers (men aged 18–21 years) who were administered placebo orsilymarin at a dose of 858 mg/day. After two months of silymarine administration, CV revealed a statisticallysignificant increase in total antioxidant capacity compared to placebo. No statistically significant changes inTBARS, SH-groups, creatininin, urea, and uric acid concentrations were found. The second group under studycomprised 49 patients with chronic renal disease during dialysis therapy. After dialysis, CV revealed a decrease oftotal antioxidant capacity in the plasma, which was equivalent to a decrease in creatinine, urea and uric acid. CV wasperformed using a system consisting of a working glassy carbon electrode, an auxiliary platinum electrode, anda reference saturated calomel electrode; a linear change of voltage of 200 mV/s was applied. CV is a simple andrelatively reliable method for assessment of body antioxidant status. It is also time and cost effective.

Keywords: Cyclic voltammetry, Plasma, Total antioxidant capacity, Silymarine, Dialysis

Received: October 12, 2001; Published: December 1, 2001  Show citation