Fottea 2015, 15(2):113-122 | DOI: 10.5507/fot.2015.013

Community structure of corticolous microalgae within a single forest stand: evaluating the effects of bark surface pH and tree species

Anna ©tifterová, Jiøí Neustupa*
Department of Botany, Faculty of Science, Charles University Prague, Benátská 2, Prague, CZ-128 01, Czech Republic; *Corresponding author e-mail: neustupa@natur.cuni.cz

Community structure of corticolous macro-epiphytes, such as lichens and mosses, usually strongly correlates with bark characteristics related to the taxonomic identity of the host tree. However, the reasons for the occurrence of similar patterns of microalgal corticolous biofilms are unclear and have rarely been investigated. In this study, we surveyed aerial phototrophic biofilms growing on the bark of Acer, Alnus, Fagus, Fraxinus and Picea trees in a single stand of a temperate forest. A flathead electrode was used to measure and evaluate the effect of bark surface pH on microalgal species richness and composition. In total, 47 distinct morphotypes were found, chiefly consisting of various coccoid green algae belonging to Trebouxiophyceae and Chlorophyceae. In addition, members of the Bacillariophyceae, Eustigmatophyceae, Streptophyta, Trentepohliales, Xanthophyceae and Cyanobacteria were also found. Bark pH and species richness of the samples were positively correlated; in the most acidic samples (from Picea and Alnus trees), only green algae occured. Aerial diatoms, Cyanobacteria, Xanthophyceae and Trentepohlia sp. were only found in the more basic samples. The species composition was significantly structured by the separated effects of host tree species, the bark pH and the sampling season. The observed non-random differences in community structure of the biofilms among host tree species indicated possible effects of deterministic processes, such as species sorting of microalgae along the environmental gradients.

Keywords: Algae and Cyanobacteria, Bark pH, Biodiversity, Corticolous biofilms, Epiphyte ecology, Trebouxiophyceae

Received: February 10, 2015; Accepted: March 30, 2015; Prepublished online: September 1, 2015; Published: October 1, 2015Show citation

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©tifterová, A., & Neustupa, J. (2015). Community structure of corticolous microalgae within a single forest stand: evaluating the effects of bark surface pH and tree species. Fottea15(2), 113-122. doi: 10.5507/fot.2015.013
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References

  1. Andersen, R.A. (2005): Algal culturing techniques. - Elsevier Academic Press, London.
  2. Barkman, J.J. (1958): Phytosociology and ecology of cryptogamic epiphytes. - Van Gorkum, Assen.
  3. Büdel, B. (2011): Eukaryotic Algae. - In: Lüttge, U.; Beck, E. & Bartels, D. (eds): Plant Desiccation Tolerance. - 45-63 pp., Springer, Berlin - Heidelberg. Go to original source...
  4. Cáceres, M.E.; Lücking, R. & Rambold, G. (2007): Phorophyte specificity and environmental parameters versus stochasticity as determinants for species composition of corticolous crustose lichen communities in the Atlantic rain forest of northeastern Brazil. - Mycological Progress 6: 117-136. Go to original source...
  5. Caruso, A. & Thor, G. (2007): Importance of different tree fractions for epiphytic lichen diversity on Picea abies and Populus tremula in mature managed boreonemoral Swedish forests. - Scandinavian Journal of Forest Research 22: 219-230. Go to original source...
  6. Colwell, R.K.; Mao, C.X. & Chang, J. (2004): Interpolating, extrapolating, and comparing incidence-based species accumulation curves. - Ecology 85: 2717-2727. Go to original source...
  7. Cutler, N.A.; Oliver, A.E.; Viles, H.A.; Ahmad, S. & Whiteley, A.S. (2013): The characterisation of eukaryotic microbial communities on sandstone buildings in Belfast, UK, using TRFLP and 454 pyrosequencing. - International Biodeterioration and Biodegradation 82: 124-133. Go to original source...
  8. Davies, L.O.; Schäfer, H.; Marshall, S.; Bramke, I.; Oliver, R.G. & Bending, G.D. (2013): Light structures phototroph, bacterial and fungal communities at the soil surface. - PLoS One 8: e69048. Go to original source...
  9. Ellis, C.J. (2012): Lichen epiphyte diversity: a species, community and trait-based review. - Perspectives in Plant Ecology 14: 131-152. Go to original source...
  10. Ettl, H. & Gärtner, G. (1995): Syllabus der Boden-, Luft- und Flechtenalgen. - Gustav Fisher Verlag, Stuttgart.
  11. Freystein, K.; Salisch, M. & Reisser, W. (2008): Algal biofilms on tree bark to monitor airborne pollutants. - Biologia 63: 866-872. Go to original source...
  12. Freystein, K. & Reisser, W. (2010): Green biofilms on tree barks: More than Just Algae. - In: Seckbach, J. & Grube, M. (eds): Symbioses and Stress. - 557-573 pp., Springer, Netherlands. Go to original source...
  13. Friedl, T. & Rybalka, N. (2012): Systematics of the green algae: A brief introduction to the current status. - In: Lüttge, U.; Beyschlag, W.; Büdel, B. & Francis, D. (eds): Progress in Botany 73. - 259-280 pp., Springer, Berlin. Go to original source...
  14. Gärtner, G. (1994): Zur Taxonomie aerophiler grüner Algenanflüge an Baumrinden. - Berichte des naturwissenschaftlich-medizinischen Vereins in Innsbruck 81: 51-59.
  15. Gonzalez-Mancebo, J.M.; Losada-Lima, A. & McAlister, S. (2003): Host specificity of epiphytic bryophyte communities of a laurel forest on Tenerife (Canary Islands, Spain). - The Bryologist 106: 383-394. Go to original source...
  16. Hájek, M.; Roleèek, J.; Cottenie, K.; Kintrová, K.; Horsák, M.; Poulíèková, A.; Hájková, P.; Fránková, M. & Dítì, D. (2011): Environmental and spatial controls of biotic assemblages in a discrete semi-terrestrial habitat: comparison of organisms with different dispersal abilities sampled in the same plots. - Journal of Biogeography 38: 1683-1693. Go to original source...
  17. Hammer, Ø.; Harper, D.A.T. & Ryan, P.D. (2001): PAST: Paleontological statistics software package for education and data analysis. Palaeontologia Electronica 4(1): 9pp. Available from: http://palaeo-electronica.org/2001_1/past/issue1_01.htm.
  18. Hänninen, O.; Ruuskanen, J. & Oksanen, J. (1993): A method for facilitating the use of algae growing on tree trunks as bioindicators of air quality. - Environmental Monitoring Assessment 28: 215-220. Go to original source...
  19. Häubner, N.; Schumann, R. & Karsten, U. (2006): Aeroterrestrial microalgae growing in biofilms on facades - response to temperature and water stress. - Microbial Ecology 51: 285-293. Go to original source...
  20. Hedenås, H.; Blomberg, P. & Ericson, L. (2007): Significance of old aspen (Populus tremula) trees for the occurrence of lichen photobionts. - Biological Conservation 135: 380-387. Go to original source...
  21. Hoffmann, L. (1989): Algae of terrestrial habitats. - Botanical Review 55: 77-105. Go to original source...
  22. Johansen, J.R. (2010): Diatoms of aerial habitats. - In: Smol, J.P. & Stoermer, E.F. (eds): The diatoms: Applications for the Environmental and Earth Sciences. - 465-472 pp., Cambridge University Press, Cambridge. Go to original source...
  23. Karsten, U. & Holzinger, A. (2014): Green algae in alpine biological soil crust communities: acclimation strategies against ultraviolet radiation and dehydration. - Biodiversity and Conservation 23: 1845-1858. Go to original source...
  24. Kitzing, C.; Pröschold, T. & Karsten, U. (2014): UV-Induced effects on growth, photosynthetic performance and sunscreen contents in different populations of the green alga Klebsormidium fluitans (Streptophyta) from alpine soil crusts. - Microbial Ecology 67: 327-340. Go to original source...
  25. Komárek, J. & Anagnostidis, K. (2005): Süßwasserflora von Mitteleuropa, Cyanoprokaryota 19/2. Teil: Oscillatoriales. - 759 pp., Elsevier, München.
  26. Kulichová, J.; ©kaloud, P. & Neustupa, J. (2014): Molecular diversity of green corticolous microalgae from two sub-Mediterranean European localities. - European Journal of Phycology 49: 345-355. Go to original source...
  27. Larsen, R.S.; Bell, J.N.B.; James, P.W.; Chimonides, P.J.; Rumsey, F.J.; Tremper, A. & Purvis, O.W. (2007): Lichen and bryophyte distribution on oak in London in relation to air pollution and bark acidity. - Environmental Pollution 146: 332-340. Go to original source...
  28. Lemes-da-Silva, N.M.; Zanini Branco, L.H. & Necchi Júnior, O. (2012): Corticolous cyanobacteria from tropical forest remnants in northwestern São Paulo State, Brazil. - Brazilian Journal of Botany 35: 169-179. Go to original source...
  29. Lewis, J.E. & Ellis, C.J. (2010): Taxon-compared with trait-based analysis of epiphytes, and the role of tree species and tree age in community composition. - Plant Ecology and Diversity 3: 203-210. Go to original source...
  30. Lopez-Bautista, J.M.; Rindi, F. & Casamatta, D. (2007): The systematics of subaerial algae. - In: Seckbach, J. (ed): Algae and cyanobacteria in extreme environments. - 599-617 pp., Springer, Netherlands. Go to original source...
  31. Luke¹ová, A. & Hoffmann, L. (1996): Soil algae from acid rain impacted forest areas of the Kru¹né hory Mts. - Vegetatio 125: 123-36. Go to original source...
  32. Lüttge, U. & Büdel, B. (2010): Resurrection kinetics of photosynthesis in desiccation-tolerant terrestrial green algae (Chlorophyta) on tree bark. - Plant Biology 12: 437-444. Go to original source...
  33. Ma, S.; Huss, V.A.; Tan, D.; Sun, X.; Chen, J.; Xie, Y. & Zhang, J. (2013): A novel species in the genus Heveochlorella (Trebouxiophyceae, Chlorophyta) witnesses the evolution from an epiphytic into an endophytic lifestyle in tree-dwelling green algae. - European Journal of Phycology 48: 200-209. Go to original source...
  34. Marmor, L. & Randlane, T. (2007): Effects of road traffic on bark pH and epiphytic lichens in Tallinn. - Folia Cryptogamica Estonica 43: 23-37.
  35. Marmor, L.; Tõrra, T. & Randlane, T. (2010): The vertical gradient of bark pH and epiphytic macrolichen biota in relation to alkaline air pollution. - Ecological Indicators 10: 1137-1143. Go to original source...
  36. Marmor, L. & Degtjarenko, P. (2014): Trentepohlia umbrina on Scots pine as a bioindicator of alkaline dust pollution. - Ecological Indicators 45: 717-720. Go to original source...
  37. McCune, B.; Rosentreter, R.; Ponzetti, J.M. & Shaw, D.C. (2000): Epiphyte habitats in an old conifer forest in western Washington, USA. - The Bryologist 103: 417-427. Go to original source...
  38. Mikhailyuk, T.I. (1999): Eusubaerial algae of Kaniv Nature Reserve (Ukraine). - Ukrayins'kyi botanichnyi zhurnal 56: 507-513.
  39. Mikhailyuk, T.I.; Tsarenko, P.M.; Nevo, E. & Wasser, S.P. (2001): Additions to the study of aerophytic eukaryotic algae of Israel. - International Journal on Algae 3: 19-39. Go to original source...
  40. Mikhailyuk, T.I.; Sluiman, H.J.; Massalski, A.; Mudimu, O.; Demchenko, E.M.; Kondratyuk, S.Y. & Friedl, T. (2008): New streptophyte algae from terrestrial habitats and an assessment of the genus Interfilum (Klebsormidiophyceae, Streptophyta). - Journal of Phycology 44: 1586-1603. Go to original source...
  41. Nakano, T.; Handa, S. & Takeshita, S. (1991): Some corticolous algae from the Taishaku-kyô Gorge, western Japan. - Nova Hedwigia 52: 427-451.
  42. Nìmcová, Y.; Eliá¹, M.; ©kaloud, P.; Hodaè, L. & Neustupa, J. (2011): Jenufa gen. nov.: A new genus of coccoid green algae (Chlorophyceae, inceartae sedis) previously recorded by environmental sequencing. - Journal of Phycology 47: 928-938. Go to original source...
  43. Neustupa, J. & ©kaloud, P. (2008): Diversity of subaerial algae and cyanobacteria on tree bark in tropical mountain habitats. - Biologia 63: 806-812. Go to original source...
  44. Neustupa, J.; Nìmcová, Y.; Veselá, J.; Steinová, J. & ©kaloud, P. (2013): Parachloroidium gen. nov. (Trebouxiophyceae, Chlorophyta), a novel genus of coccoid green algae from subaerial corticolous biofilms. - Phycologia 52: 411-421. Go to original source...
  45. Neustupa, J. & ©tifterová, A. (2013): Distribution patterns of subaerial corticolous microalgae in two European regions. - Plant Ecology and Evolution 146: 279-289. Go to original source...
  46. Oksanen, J.; Blanchet, F.G.; Kindt, R.; Legendre, P.; Minchin, P.R.; O'Hara, R.B.; Simpson, G.L.; Solymos, P.; Stevens, M.H.H. & Wagner, H. (2011): Vegan: Community Ecology Package. R package version 2.0-0 [online]. Available from http://CRAN.R-project.org/package=vegan.
  47. Poikolainen, J.; Lippo, H.; Hongisto, M.; Kubin, E.; Mikkola, K. & Lindgren, M. (1998): On the abundance of epiphytic green algae in relation to the nitrogen concentrations of biomonitors and nitrogen deposition in Finland. - Environmental Pollution 102: 85-92. Go to original source...
  48. R Development Core Team (2013): R: A language and environment for statistical computing, ver. 2.15.3. - R Foundation for Statistical Computing, Vienna.
  49. Rambo, T.R. (2010): Structure and composition of corticolous epiphyte communities in a Sierra Nevada old-growth mixed-conifer forest. - The Bryologist 113: 55-71. Go to original source...
  50. Ress, J.A. & Lowe, R.L. (2013): Contrast and comparison of aerial algal communities from two distinct regions in the U.S.A., the Great Smoky Mountains National Park (TN) and the Lake Superior region. - Fottea 13: 165-172. Go to original source...
  51. Rindi, F.; Allali, H.A.; Lam, D.W. & Lopéz-Bautista, J.M. (2009): An overview of the biodiversity and biogeography of terrestrial green algae. - In: Rescigno, V. & Maletta, S. (eds): Biodiversity Hotspots. - 105-122 pp., Nova Science Publishers, New York.
  52. Rindi, F.; Mikhailyuk, T.I.; Sluiman, H.J.; Friedl, T. & López-Bautista, J.M. (2011): Phylogenetic relationships in Interfilum and Klebsormidium (Klebsormidiophyceae, Streptophyta). - Molecular Phylogenetics and Evolution 58: 218-231. Go to original source...
  53. Scarborough, A.R.; Keller, H. W. & Ely, J.S. (2009): Species assemblages of tree canopy myxomycetes related to bark pH. - Castanea 74: 93-104. Go to original source...
  54. Schmidt, J.; Kricke, R. & Feige, G. B. (2001): Measurements of bark pH with a modified flathead electrode. - The Lichenologist 33: 456-460. Go to original source...
  55. Sharma, N.K.; Rai, A.K.; Singh, S. & Brown, R.M. (2007): Airborne algae: their present status and relevance. - Journal of Phycology 43: 615-627. Go to original source...
  56. Sharma, N.K. & Singh, S. (2010): Differential aerosolization of algal and cyanobacterial particles in the atmosphere. - Indian Journal of Microbiology 50: 468-473. Go to original source...
  57. Spier, L.; Van Dobben, H. & Van Dort, K. (2010): Is bark pH more important than tree species in determining the composition of nitrophytic or acidophytic lichen floras? - Environmental Pollution 158: 3607-3611. Go to original source...
  58. Studlar, S.M. (1982): Host specificity of epiphytic bryophytes near Mountain Lake, Virginia. - The Bryologist 85: 27-50. Go to original source...
  59. Valová, M. & Bieleszová, S. (2008): Interspecific variations of bark´s water storage capacity of chosen types of trees and the dependance on occurance of epiphytic mosses. - GeoScience Engineering 54: 45-51.