Fottea 2021, 21(2):235-246 | DOI: 10.5507/fot.2021.008

Multiple phylogenies reveal a true taxonomic position of Dulcicalothrix alborzica sp. nov. (Nostocales, Cyanobacteria)

Bahareh Nowruzi1, Sergei Shalygin2, *
1 Department of Biology, Science and Research Branch, Islamic Azad University, Daneshgah Blvd, Simon Bulivar Blvd, Tehran, Iran
2 Plant and Environmental Sciences Department, New Mexico State University, 945 College Drive, Las Cruces, New Mexico, 88003 USA; *Corresponding author phone: +1(575)339-99-09, e-mail: sshalygin18@jcu.edu

A new species of heterocytous cyanobacterium Dulcicalothrix alborzica sp. nov. (Calotrichaceae) was isolated from Iran and described following a polyphasic approach. Morphological examination indicated that the strain, initially called "Alborzica", belonged to the genus Calothrix. Nonetheless, 16S rRNA gene analysis showed that "Alborzica" were within the Dulcicalothrix cluster. In order to confirm the taxonomic position of this new taxon, rbcL and rpoC1 phylogenies were inferred. Results confirmed identity of "Alborzica" supporting the 16S rRNA phylogeny. Analysis of the secondary structures of 16S-23S Internal Transcribed Spacer (ITS) revealed that "Alborzica" had unique structure compared with known Dulcicalothrix spp. Therefore, based on the morphology and thorough molecular analysis, "Alborzica'' should be considered a novel species in the genus Dulcicalothrix.

Keywords: new species, polyphasic approach, rbcL, rpoC1, 16S-23S ITS

Received: January 10, 2021; Revised: May 6, 2021; Accepted: May 6, 2021; Prepublished online: September 6, 2021; Published: October 13, 2021Show citation

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Nowruzi, B., & Shalygin, S. (2021). Multiple phylogenies reveal a true taxonomic position of Dulcicalothrix alborzica sp. nov. (Nostocales, Cyanobacteria). Fottea, 21(2), 235-246. doi: 10.5507/fot.2021.008
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    References

    1. Anagnostidis, K. & Komárek, J. (1985): Modern approach to the classification system of cyanophytes. 1 - Introduction. - Algological Studies 38/39: 291-302.
    2. Becerra-Absalón, I.; Johansen, J.R.; Osorio-Santos, K. & Montejano, G. (2020): Two new Oculatella (Oculatellaceae, Cyanobacteria) species in soil crusts from tropical semi-arid uplands of México. - Fottea 20: 160-70. Go to original source...
    3. Berrendero Gómez, E.; Johansen, J.R.; Kaštovský, J.; Bohunická, M. & Čapková, K. (2016): Macrochaete gen. nov.(Nostocales, Cyanobacteria), a taxon morphologically and molecularly distinct from Calothrix. - J. Phycol. 52: 638-55. Go to original source...
    4. Berrendero, E.; Perona, E. & Mateo, P. (2008): Genetic and morphological characterization of Rivularia and Calothrix (Nostocales, Cyanobacteria) from running water. - Int. J. Syst. Evol. Microbiol. 58: 447-60. Go to original source...
    5. Berrendero, E.; Perona, E. & Mateo, P. (2011): Phenotypic variability and phylogenetic relationships of the genera Tolypothrix and Calothrix (Nostocales, Cyanobacteria) from running water. - Int. J. Syst. Evol. Microbiol. 61: 3039-51. Go to original source...
    6. Cai, F.; Peng, X. & Li, R. (2020): Violetonostoc minutum gen. et sp. nov. (Nostocales, Cyanobacteria) from a rocky substrate in China. - Algae 35: 1-16. Go to original source...
    7. Casamatta, D.A.; Villanueva, C.D.; Garvey, A.D.; Stocks, H.S.; Vaccarino, M.; Dvořák, P.; Hašler, P. & Johansen, J.R. (2020): Reptodigitus chapmanii (Nostocales, Hapalosiphonaceae) gen. nov.: a unique nostocalean (Cyanobacteria) genus based on a polyphasic approach. - J. Phycol. 56: 425-36. Go to original source...
    8. Castenholz, R. W. & Norris, T. B. (2005): Revisionary concepts of species in the Cyanobacteria and their applications. - Algological Studies 117: 53-69. Go to original source...
    9. Davydov, D.; Shalygin, S. & Vilnet, A. (2020): New cyanobacterium Nodosilinea svalbardensis sp. nov.(Prochlorotrichaceae, Synechococcales) isolated from alluvium in Mimer river valley of the Svalbard archipelago. - Phytotaxa 442: 61-79. Go to original source...
    10. Desikachary, T. V. (1959): Cyanophyta. - 686 pp., New Delhi: Indian Council of Agricultural Research.
    11. Edgar, R. C. (2004): MUSCLE: multiple sequence alignment with high accuracy and high throughput. - Nucl. Acids Res. 32: 1792-7. Go to original source...
    12. Galhano, V.; De Figueiredo, D.R.; Alves, A.; Correia, A.; Pereira, M.J.; Gomes-Laranjo, J. & Peixoto, F. (2011): Morphological, biochemical and molecular characterization of Anabaena, Aphanizomenon and Nostoc strains (Cyanobacteria, Nostocales) isolated from Portuguese freshwater habitats. - Hydrobiologia 663: 187-203. Go to original source...
    13. Garcia-Pichel, F.; Loza, V.; Marusenko, Y.; Mateo, P. & Potrafka, R.M. (2013): Temperature drives the continental-scale distribution of key microbes in topsoil communities. - Science 340: 1574-7. Go to original source...
    14. González-Resendiz, L.; Johansen, J.R.; Alba-Lois, L.; Segal-Kischinevzky, C.; Escobar-Sanchez, V.; Jimenez Garcia, L.F.; Hauer, T. & León-Tejera, H. (2018): Nunduva, a new marine genus of Rivulariaceae (Nostocales, Cyanobacteria) from marine rocky shores. - Fottea 18: 86-105. Go to original source...
    15. Gould, S. B.; Waller, R. F. & McFadden, G. I. (2008): Plastid evolution. - Ann. Rev. Plant Biol. 59: 491-517. Go to original source...
    16. Gugger, M.; Lyra, C.; Henriksen, P.; Coute, A.; Humbert, J.F. & Sivonen, K. 2002. Phylogenetic comparison of the cyanobacterial genera Anabaena and Aphanizomenon. - Int. J. Syst. Evol. Microbiol. 52: 1867-80. Go to original source...
    17. Heidari, F.; Riahi, H.; Yousefzadi, M. & Shariatmadari, Z. (2013): Morphological and phylogenetic diversity of cyanobacteria in four hot springs of Iran. - Iran. J. Bot. 19: 162-72.
    18. Heidari, F.; Zima, J.; Riahi, H. & Hauer, T. (2018): New simple trichal cyanobacterial taxa isolated from radioactive thermal springs. - Fottea 18: 137-49. Go to original source...
    19. Hentschke, G. S.; Johansen, J. R.; Piestrasiak, N.; Fiore, M. F.; Rigonato, J.; Sant'Anna, C. L. & Komárek, J. (2016): Phylogenetic placement of Dapisostemon gen. nov. and Streptostemon, two tropical heterocytous genera (Cyanobacteria). - Phytotaxa 245:129-43. Go to original source...
    20. Herdman M. & Rippka R. (2021): Cyanobacterial phylogenetic tree. - http://cyanophylogeny.scienceontheweb.net
    21. Hoffmann, L.; Komárek, J. & Kaštovskỳ, J. (2005): System of cyanoprokaryotes (Cyanobacteria) - state in 2004. - Algological Studies 117: 95-115. Go to original source...
    22. Hongmei, J.; Aitchison, J.C.; Lacap, D.C.; Peerapornpisal, Y.; Sompong, U. & Pointing, S.B. (2005): Community phylogenetic analysis of moderately thermophilic cyanobacterial mats from China, the Philippines and Thailand. - Extremophiles 9: 325-32. Go to original source...
    23. Johansen, J. R. & Casamatta, D. A. (2005): Recognizing cyanobacterial diversity through adoption of a new species paradigm. - Algological Studies 117: 71-93. Go to original source...
    24. Johansen, J.R.; Kovacik, L.; Casamatta, D.A.; Iková, K.F. & Kaštovský, J. (2011): Utility of 16S-23S ITS sequence and secondary structure for recognition of intrageneric and intergeneric limits within cyanobacterial taxa: Leptolyngbya corticola sp. nov.(Pseudanabaenaceae, Cyanobacteria). - Nova Hedwigia 92: 283-302. Go to original source...
    25. Jöhnk, K.D.; Brüggemann, R.; Rücker, J.; Luther, B.; Simon, U.; Nixdorf, B. & Wiedner, C. (2011): Modelling life cycle and population dynamics of Nostocales (cyanobacteria). - Environ. Model. Softw. 26: 669-77. Go to original source...
    26. Komárek, J. & Kaštovský, J. (2003): Coincidences of structural and molecular characters in evolutionary lines of cyanobacteria. - Algological Studies 109: 305-25. Go to original source...
    27. Komárek, J. (2013): Cyanoprokaryota: 3. Teil: Heterocytous Genera. - In: Büdel, B.; Gärtner, G.; Krienitz, L. & Schagerl, M. (eds): Süßwasserflora von Mitteleuropa, Bd. 19/3. - 1130 pp., Springer Specktrum, Berlin. Go to original source...
    28. Komárek, J.; Kaštovský, J.; Mareš, J. & Johansen, J.R. (2014): Taxonomic classification of cyanoprokaryotes (cyanobacterial genera) 2014, using a polyphasic approach. - Preslia 86: 295-335.
    29. Krienitz, L.; Dadheech, P. K. & Kotut, K. (2013): Mass developments of the cyanobacteria Anabaenopsis and Cyanospira (Nostocales) in the soda lakes of Kenya: ecological and systematic implications. - Hydrobiologia 703: 79-93. Go to original source...
    30. Kumar, S.; Stecher, G.; Li, M.; Knyaz, C.; Tamura, K. & Battistuzzi, F. U. (2018): MEGA X: molecular evolutionary genetics analysis across computing platforms. - Mol. Biol. Evol. 35: 1547-1549. Go to original source...
    31. Mareš, J.; Strunecký, O.; Bučinská, L. & Wiedermannová, J. (2019): Evolutionary Patterns of Thylakoid Architecture in Cyanobacteria. - Front. Microbiol. 10:1-22. Go to original source...
    32. Minh, B.Q.; Nguyen, M.A.T. & von Haeseler, A. (2013): Ultrafast approximation for phylogenetic bootstrap. - Mol. Biol. Evol. 30: 1188-1195. Go to original source...
    33. Mishra, S.; Bhargava, P.; Adhikary, S.P.; Pradeep, A. & Rai, L.C. (2015): Weighted morphology: a new approach towards phylogenetic assessment of Nostocales (Cyanobacteria). - Protoplasma 252: 145-163 Go to original source...
    34. Nguyen, L.T.; Schmidt, H.A.; Von Haeseler, A. & Minh, B.Q. (2015): IQ-TREE: a fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies. - Mol. Biol. Evol. 32: 268-274. Go to original source...
    35. Nowruzi, B. & Ahmadimoghadam, A. (2006): Two new records of heterocystus cyanobacteria (Nostocaceae) from paddy fields of Golestan Province. - Iran. J. Bot. 11: 170-173.
    36. Nowruzi, B. & Blanco, S. (2019): In silico identification and evolutionary analysis of candidate genes involved in the biosynthesis methylproline genes in cyanobacteria strains of Iran. - Phytochem. Lett. 29: 199-211. Go to original source...
    37. Nowruzi, B. & Lorenzi, A.S. (2021): Characterization of a potentially microcystin-producing Fischerella sp. isolated from Ajigol wetland of Iran. - S. Afr. J. Bot. 137: 423-33. Go to original source...
    38. Nowruzi, B.; Blanco, S. & Nejadsattari, T. (2018): Chemical and molecular evidences for the poisoning of a duck by anatoxin-a, nodularin and cryptophycin at the coast of Lake Shoormast (Mazandaran province, Iran). - Int. J. Algae. 20: 359-76. Go to original source...
    39. Nowruzi, B.; Khavari-Nejad, R.A.; Nejadsattari, T.; Sivonen, K. & Fewer, D. (2017): A proposal for the unification of two cyanobacterial strains of Nostoc as the same species. - Rostaniha 17: 161-72.
    40. Nowruzi, B.; Khavari-Nejad, R.A.; Sivonen, K.; Kazemi, B.; Najafi, F. & Nejadsattari, T. (2012): Identification and toxigenic potential of a Nostoc sp. - Algae 27: 303-13. Go to original source...
    41. Řeháková, K.; Johansen, J.R.; Casamatta, D.A.; Xuesong, L. & Vincent, J. (2007): Morphological and molecular characterization of selected desert soil cyanobacteria: three species new to science including Mojavia pulchra gen. et sp. nov. - Phycologia 46: 481-502. Go to original source...
    42. Rippka, R.; Deruelles, J.; Waterbury, J.B.; Herdman, M. & Stanier, R.Y. (1979): Generic assignments, strain histories and properties of pure cultures of cyanobacteria. - Microbiology 111: 1-61. Go to original source...
    43. Roeselers, G.; Stal, L.J.; van Loosdrecht, M.C. & Muyzer, G. (2007): Development of a PCR for the detection and identification of cyanobacterial nifD genes. - J. Microbiol. Methods 70: 550-556. Go to original source...
    44. Saraf, A.; Suradkar, A.; Dawda, H.G.; Gaysina, L.A.; Gabidullin, Y.; Kumat, A.; Behere, I.; Kotulkar, M.; Batule, P. & Singh, P. (2019): Phylogenetic complexities of the members of Rivulariaceae with the re-creation of the family Calotrichaceae and description of Dulcicalothrix necridiiformans gen nov., sp nov., and reclassification of Calothrix desertica. - FEMS Microbiol. Lett. 366: p.fnz219. Go to original source...
    45. Shalygin, S.; Pietrasiak, N.; Gomez, F.; Mlewski, C.; Gerard, E. & Johansen, J.R. (2018): Rivularia halophila sp. nov. (Nostocales, Cyanobacteria): the first species of Rivularia described with the modern polyphasic approach. - Eur. J. Phycol. 53: 537-548. Go to original source...
    46. Shalygin, S.; Shalygina, R.; Johansen, J.R.; Pietrasiak, N.; Berrendero Gómez, E.; Bohunická, M.; Mareš, J. & Sheil, C.A. (2017): Cyanomargarita gen. nov.(Nostocales, Cyanobacteria): convergent evolution resulting in a cryptic genus. - J. Phycol. 53: 762-777. Go to original source...
    47. Siegesmund, M.A.; Johansen, J.R.; Karsten, U. & Friedl, T. (2008): Coleofasciculus gen. nov.(Cyanobacteria): morphological and molecular criteria for revision of the genus Microcoleus Gomont. - J. Phycol. 44: 1572-1585. Go to original source...
    48. Sihvonen, L.M.; Lyra, C.; Fewer, D.P.; Rajaniemi-Wacklin, P.; Lehtimäki, J.M.; Wahlsten, M. & Sivonen, K. (2007): Strains of the cyanobacterial genera Calothrix and Rivularia isolated from the Baltic Sea display cryptic diversity and are distantly related to Gloeotrichia and Tolypothrix. - FEMS Microbiol. Ecol. 61: 74-84. Go to original source...
    49. Taton, A.; Grubisic, S.; Ertz, D.; Hodgson, D.A.; Piccardi, R.; Biondi, N.; Tredici, M.R.; Mainini, M.; Losi, D.; Marinelli, F. & Wilmotte, A. (2006): Polyphasic study of Antarctic cyanobacterial strains. - J. Phycol. 42: 1257-1270. Go to original source...
    50. Thomazeau, S.; Houdan-Fourmont, A.; Couté, A.; Duval, C.; Couloux, A.; Rousseau, F. & Bernard, C. (2009): The contribution of sub-Saharan African strains to the phylogeny of cyanobacteria: focusing on the Nostocaceae family (Nostocales, Cyanobacteria). - J. Phycol. 46: 564-579 Go to original source...
    51. Turland, N.J.; Wiersema, J.H.; Barrie, F.R.; Greuter, W.; Hawksworth, D.L.; Herendeen, P.S.; Knapp, S.; Kusber, W.-H.; Li, D.-Z.; Marhold, K.; May, T.W.; McNeill, J.; Monro, A.M.; Prado, J.; Price, M.J. & Smith, G.F. (eds.) (2018): International Code of Nomenclature for algae, fungi, and plants (Shenzhen Code) adopted by the Nineteenth International Botanical Congress Shenzhen, China, July 2017. Regnum Vegetabile. - 159 pp., Koeltz Botanical Books, Glashütten. Go to original source...
    52. Werner, V.R.; Laughinghouse IV, H.D.; Fiore, M.F.; Sant'Anna, C.L.; Hoff, C., de Souza Santos, K.R.; Neuhaus, E.B.; Molica, R.J.R.; Honda, R.Y. & Echenique, R.O. (2012): Morphological and molecular studies of Sphaerospermopsis torques-reginae (Cyanobacteria, Nostocales) from South American water blooms. - Phycologia 51: 228-38. Go to original source...
    53. Wilmotte, A. & Golubic, S. (1991): Morphological and genetic criteria in the taxonomy of Cyanophyta Cyanobacteria. - Arch. Hydrobiol. 92: 1-24.
    54. Wilmotte, A. (1994): Molecular Evolution and Taxonomy of the Cyanobacteria. - In: Bryant, D. A. (ed.): The Molecular Biology of Cyanobacteria. - pp. 1-25, Springer, Dordrecht. Go to original source...
    55. Zapomělová, E.; Řeháková, K.; Jezberová, J. & Komárková, J. (2010): Polyphasic characterization of eight planktonic Anabaena strains (Cyanobacteria) with reference to the variability of 61 Anabaena populations observed in the field. ‒ Hydrobiologia 639: 99-113. Go to original source...
    56. Zimba, P.V.; Shalygin, S.; Huang, I.S.; Momčilović, M. & Abdulla, H. (2021): A new boring toxin producer-Perforafilum tunnelli gen. & sp. nov. (Oscillatoriales, Cyanobacteria) isolated from Laguna Madre, Texas, USA. ‒ Phycologia 60: 10-24 Go to original source...
    57. Zuker, M. (2003): Mfold web server for nucleic acid folding and hybridization prediction. ‒ Nucl. Acids Res. 31: 3406-15. Go to original source...

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