Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2007, 151(2):247-249 | DOI: 10.5507/bp.2007.041

Use of glycogen phosphorylase BB measurement with POCT in the diagnosis of acute coronary syndromes. A comparison with the ELISA method

David Stejskal^a, Borek Lacnak^a, Libor Jedelsky^a, Leona Stepanova^a, Jitka Proskova^a, Pavlina Solichova^a, Lenka Kadalova^a, Marie Janosova^a, Petra Seitlova^a, Michal Karpisek^b, Ludek Sprongl^c

^a Department of Laboratory Medicine and Department of Internal Medicine, Sternberk Hospital, Czech Republic

^b Institute of Human Pharmacology and Toxicology, Faculty of Pharmaceutical Sciences, Veterinary and Pharmaceutical University Brno, Czech Republic

^c Central Laboratory, Sumperk Hospital, Czech Republic

Background: Glycogen Phosphorylase BB (GPBB) is considered an early and specific marker of myocardial necrosis and ischemia. A POCT kit GPBB for diagnostic use has recently been approved.

Aim: an evaluation of the correspondence of qualitative POCT GBPP measurements with ELISA test results. Material and methodology: 20 individuals with non-ST elevation myocardial infarction (non-STEMI) and 20 probands without acute coronary syndrome (ACS) were tested. GPBB (POCT, ELISA) in venous plasma (lithium-heparin) was assayed in all probands.

Results: individuals with non-STEMI had significantly higher GPBB ELISA values (32.3 vs. 6.1 μg/l; p < 0.01). GPBB sensitivity and specificity for non-STEMI presence 6 hours after chest pain generation were 100 %. No proband was classified in a different subgroup with POCT of GPBB (positive/negative). GPBB POCT correlate with a non- STEMI diagnosis (χ² 36.1; p <0.01).

Conclusion: GPBB POCT measurement is comparable with ELISA test results. GPBB analysis could expand the diagnostic palette in the first hours after the onset of acute coronary syndrome.

Keywords: Acute coronary syndrome, Myocardial ischemia, Non-STEMI, Coronary by-pass, Laboratory markers of myocardial ischemia, CAD, GPBB, POCT, ELISA

Received: August 21, 2007; Accepted: November 10, 2007; Published: December 1, 2007  Show citation