Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2012, 156(4):348-353 | DOI: 10.5507/bp.2012.039

Carriage of ESBL- and AmpC-positive Enterobacteriaceae in the gastrointestinal tract of community subjects and hospitalized patients in the Czech Republic

Vendula Husickovaa, Luboslava Cekanovaa, Magdalena Chromab, Miroslava Htoutou-Sedlakovaa, Kristyna Hricovaa, Milan Kolara
a Department of Microbiology, Faculty of Medicine and Dentistry, Palacky University Olomouc, Czech Republic
b Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University Olomouc

Aim: The study aimed at analyzing ESBL- and AmpC-positive Enterobacteriaceae in the gastrointestinal tracts of university hospital inpatients and persons from the Olomouc Region community, and comparing the results with data from 2007.

Methods: Bacteria were isolated from rectal swabs inoculated onto the ChromIDTM ESBL selective medium (bioMérieux). Production of ESBL-type beta-lactamases was confirmed by the modified double-disk synergy test and AmpC enzyme production was detected by the AmpC disk test. ESBL- and AmpC-positive isolates were subjected to basic genetic analysis aimed at detecting the blaTEM, blaSHV, blaCTX-M and blaAmpC genes.

Results: Over the study period (1 March 2010 - 1 May 2010), a total of 1,279 rectal swabs (70.4% of community subjects) were analyzed on the above medium. The prevalence rates of ESBL-positive Enterobacteriaceae were 8.2% in hospitalized patients and 3.2% in community subjects. Production of the AmpC enzyme was detected in 1.1% of bacterial isolates from the community and in one (0.3%) hospital isolate. Among ESBL, the most frequent genes encoding enzymes were from the CTX-M-1-like genes. Detected AmpC beta-lactamases belonged to the CIT, DHA and EBC groups.

Conclusion: When compared with the year 2007, the rates of carriers of ESBL-positive bacteria increased in both hospitalized patients (from 3% to 8%) and community subjects (from 1% to 3%) in 2010. Given the fact that production of extended-spectrum beta-lactamases is clinically significant, knowing the epidemiological situation is very important for selecting adequate antibiotic therapy.

Keywords: ESBL, AmpC, GIT, carriage

Received: August 29, 2011; Accepted: April 4, 2012; Prepublished online: May 25, 2012; Published: December 12, 2012  Show citation

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Husickova, V., Cekanova, L., Chroma, M., Htoutou-Sedlakova, M., Hricova, K., & Kolar, M. (2012). Carriage of ESBL- and AmpC-positive Enterobacteriaceae in the gastrointestinal tract of community subjects and hospitalized patients in the Czech Republic. Biomedical papers, 156(4), 348-353. doi: 10.5507/bp.2012.039
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