Fottea 2021, 21(1):8-15 | DOI: 10.5507/fot.2020.012

Mychonastes frigidus sp. nov. (Sphaeropleales/Chlorophyceae), a new species described from a mountain stream in the Subpolar Urals (Russia)

Elena Patova1*, Irina Novakovskaya1, Nikita Martynenko2, Evgeniy Gusev2, Maxim Kulikovskiy2
1 Institute of Biology FRC Komi SC UB RAS, Kommunisticheskaya Street 28, Syktyvkar, 167982, Russia; *Corresponding authore-mail: patova@ib.komisc.ru
2 К.А. Timiryazev Institute of Plant Physiology RAS, Botanicheskaya Street 35, Moscow, 127276 Russia

This paper describes a new species from the Class Chlorophyceae, Mychonastes frigidus sp. nov., isolated from a cold-water mountain stream in the north of Russia (Subpolar Ural). The taxon is described using morphological and molecular methods. Mychonastes frigidus sp. nov. belongs to the group of species of the genus Mychonastes with spherical single cells. Comparison of ITS2 rDNA sequences and its secondary structures combined with the compensatory base changes approach confirms the separation between Mychonastes frigidus sp. nov and other species of the genus. Mychonastes frigidus sp. nov. represents a cryptic species that can only be reliably identified using molecular data.

Keywords: Mychonastes, new species, SSU rDNA, ITS2 rDNA secondary structure, CBC approach, Subpolar Urals

Received: April 2, 2020; Accepted: September 2, 2020; Prepublished online: March 2, 2021; Published: April 1, 2021Show citation

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Patova, E., Novakovskaya, I., Martynenko, N., Gusev, E., & Kulikovskiy, M. (2021). Mychonastes frigidus sp. nov. (Sphaeropleales/Chlorophyceae), a new species described from a mountain stream in the Subpolar Urals (Russia). Fottea, 21(1), 8-15. doi: 10.5507/fot.2020.012
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    References

    1. Andersen, R.A. (2005): Algal Culturing Techniques. - 589 pp., Elsevier/Academic, Burlington/San & Diego/London. DOI: 10.1016/B978-012088426-1/50007-X. Go to original source...
    2. Andreyeva, V.M. (1998): Soil and aerophilic green algae (Chlorophyta: Tetrasporales, Chlorococcales, Chlorosarcinales). - 352 pp., Nauka, St. Petersburg.
    3. Ankenbrand, M.J.; Keller, A.; Wolf, M.; Schultz, J. & Förster, F. (2015): ITS2 database V: Twice as much. - Molecular Biology and Evolution 32: 3030 -3032. DOI: 10.1093/molbev/msv174. Go to original source...
    4. Byun, Y. & Han, K. (2006): PseudoViewer: web application and webservice for visualizing RNA pseudoknots and secondary structures. - Nucleic Acids Research 34: 416-422. DOI: 10.1093/nar/gkl210. Go to original source...
    5. Caisová, L.; Marin, B. & Melkonian, M. (2013): A consensus secondary structure of ITS2 in the Chlorophyta identified by phylogenetic reconstruction. - Protist 164: 482-496. DOI: 10.1016/j.protis.2013.04.005. Go to original source...
    6. Coleman, A.W. (2000): The significance of a coincidence between evolutionary landmarks found in mating affinity and a DNA sequence. - Protist 151: 1-9. DOI: 10.1078/1434-4610-00002. Go to original source...
    7. Coleman, A.W. (2009): Is there a molecular key to the level of "biological species" in eukaryotes? A DNA guide. - Molecular phylogenetics and evolution 50: 197-203. DOI: 10.1016/j.ympev.2008.10.008. Go to original source...
    8. Degteva, S.V. (2016): Flora, licheno and mycobiota especially protected landscapes of the Kosyu and Bolshaja Synya river basins (Subpolar Urals, national park "Yugyd va"). - 483 pp., KMK scientific partnership, Moscow.
    9. Eddy, S.R. (1998): Profile hidden Markov models. - Bioinformatics (Oxford, England) 14: 755-763. DOI: 10.1093/bioinformatics/14.9.755. Go to original source...
    10. Fawley, M.W.; Fawley, K.P. & Owen, H.A. (2005): Diversity and ecology of small coccoid green algae from Lake Itasca, Minnesota, USA, including Meyerella planktonica, gen. et sp. nov. - Phycologia 44: 35-48. DOI: 0.2216/0031-8884(2005)44[35:DAEOSC]2.0.CO;2. Go to original source...
    11. Guiry, M.D. & Guiry, G.M. (2020): AlgaeBase. World-wide electronic publication, National University of Ireland, Galway. http://www.algaebase.org; searched on 18 May 2020.
    12. Hanagata, N.; Malinsky-Rushansky, N. & Dubinsky, Z. (1999): Eukaryotic picoplankton, Mychonastes homosphaera (Chlorophyceae, Chlorophyta), in Lake Kinneret, Israel. - Phycological Research 47: 263-269. DOI: 10.1046/j.1440-1835.1999.00176.x. Go to original source...
    13. Hindák, F. (1978): New taxa and reclassifications in the Chlorococcales (Chlorophyceae). - Preslia, Praha 50: 97-109.
    14. Hindák, F. (1984): Studies on the chlorococcal algae (Chlorophyceae). III. - Biologické Práce 30/1: 1-308.
    15. Hindák, F. (1988a): Studies on the chlorococcal algae (Chlorophyceae). IV. - Biologické Práce 34(1/2): 1-264.
    16. Hindák, F. (1988b): Einige kokkale Grünalgen aus den Kärtner Seen, Österreich. - Archiv für Hydrobiologie 79: 446-501.
    17. Kalina, T. & Puncŏchárŏvá, M. (1987): Taxonomy of the subfamily Scotiellocystoideae Fott 1976 (Chlorellaceae, Chlorophyceae). - Archiv für Hydrobiologie 73: 473-521.
    18. Keller, A.; Schleicher, T.; Schultz, J.; Müller, T.; Dandekar, T. & Wolf, M. (2009): 5.8S-28S rRNA interaction and HMM-based ITS2 annotation. - Gene 430: 50-57. DOI: 10.1016/j.gene.2008.10.012. Go to original source...
    19. Kolesnik, S.V. (1964): Atlas of the Komi Republic. - 112 pp., Main Department of Geodesy and Cartography of the State Geological Committee of the USSR, Moscow.
    20. Komárek, J. & Fott, B. (1983): Chlorophyceae (Grünalgen) Ordnung: Chlorococcales. - In: Huber-Pestalozzi, G. (ed.): Das Phytoplankton des Süßwassers, 7. Teil, 1. - 1044 pp., Hälfte, Schweizerbart'sche Verlagsbuchhandlung (Nägele u. Obermiller), Stuttgart.
    21. Krienitz, L.; Bock, C.; Dadheech, P.K. & Pröschold, T. (2011): Taxonomic reassessment of the genus Mychonastes (Chlorophyceae, Chlorophyta) including the description of eight new species. - Phycologia 50: 89-106. DOI: 10.2216/10-15.1. Go to original source...
    22. Krienitz, L.; Huss, V.A.R. & Hümmer, C. (1996): Picoplanktonic Choricystis species (Chlorococcales, Chlorophyta) and problems surrounding the morphologically similar "Nannochloris-like algae". - Phycologia 35: 332-341. DOI: 10.2216/i0031-8884-35-4-332.1. Go to original source...
    23. Krienitz, L.; Takeda, H. & Hepperle, D. (1999): Ultrastructure, cell wall composition, and phylogenetic position of Pseudodictyosphaerium jurisii (Chlorococcales, Chlorophyta) including a comparison with other picoplanktonic green algae. - Phycologia 38: 100-107. DOI: 10.2216/i0031-8884-38-2-100.1. Go to original source...
    24. Kumar, S.; Stecher, G.; Li, M.; Knyaz, C. & Tamura, K. (2018): MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms. - Molecular Biology and Evolution 35: 1547-1549. DOI: 10.1093/molbev/msy096. Go to original source...
    25. Margulis, B.L.; Hinkle, G.; McKhann, H. & Moynihan, B. (1988): Mychonastes desiccatus Brown sp. Nova (Chlorococcales, Chlorophyta) - an intertidal alga forming achlorophyllous desiccation-resistant cysts. - Archiv für Hydrobiologie 78: 425-446.
    26. McFadden, G.I. & Melkonian, M. (1986): Use of Hepes buffer for microalgal culture media and fixation for electron microscopy. - Phycologia 25: 551-557. DOI: 10.2216/i0031-8884-25-4-551.1. Go to original source...
    27. Medlin, L.; Elwood, H.J.; Stickel, S. & Sogin, M.L. (1988): The characterization of enzymatically amplified eukaryotic 16S-like rRNA-coding regions. - Gene 71: 491-499. DOI: 10.1016/0378-1119(88)90066-2. Go to original source...
    28. Müller, T.; Philippi, N.; Dandekar, T.; Schultz, J. & Wolf, M. (2007): Distinguishing species. - RNA 13: 1469-1472. DOI: 10.1261/rna.617107. Go to original source...
    29. Patova, E.N. (2010): Biodiversity of aquatic and terrestrial ecosystems of the Kozhym River Basin (Northern part of the national park). - 192 pp., Syktyvkar.
    30. Patova, E.N. & Novakovskaya, I.V. (2018): Soil algae of the Northeastern European Russia. - Novosti sistematiki nizshikh rastenii 52: 311-353. DOI: 10.31111/nsnr/2018.52.2.311. Go to original source...
    31. Posada, D. (2006): Modeltest Server: a web-based tool for the statistical selection of models of nucleotide substitution online. - Nucleic Acids Res. 34: 700-703. DOI: 10.1093/nar/gkl042. Go to original source...
    32. Pruesse, E., Peplies, J. & Glöckner, F.O. (2012): SINA: accurate high-throughput multiple sequence alignment of ribosomal RNA genes. - Bioinformatics 28: 1823-1829. DOI: 10.1093/bioinformatics/bts252. Go to original source...
    33. Ramos, G.J.P.; Bicudo, C.E. de M. & Moura, C.W. do N. (2015): Trebouxiophyceae (Chlorophyta) from Marimbus Wetlands, Chapada Diamantina, Bahia, Brazil. - Iheringia Série Botanica 70: 57-72.
    34. Ronquist, F. & Huelsenbeck, J.P. (2003): MrBayes 3: Bayesian phylogenetic inference under mixed models. - Bioinformatics 19: 1572-1574. DOI: 10.1093/bioinformatics/btg180. Go to original source...
    35. Schultz, J.; Maisel, S.; Gerlach, D.; Müller, T. & Wolf, M. (2005): A common core of secondary structure of the internal transcribed spacer 2 (ITS2) throughout the Eukaryota. - RNA 11: 361-364. DOI: 10.1261/rna.7204505. Go to original source...
    36. Seibel, P.N.; Müller, T.; Dandekar, T.; Schultz, J. & Wolf, M. (2006): 4SALE - a tool for synchronous RNA sequence and secondary structure alignment and editing. - BMC bioinformatics 7: 498. DOI: 10.1186/1471-2105-7-498. Go to original source...
    37. Simpson, P.D. & Van Valkenburg, S.D. (1978): The ultrastructure of Mychonastes ruminatus gen. et sp. nov., a new member of the Chlorophyceae isolated from brackish water. - British Phycology Journal 13: 117-30. DOI: 10.1080/00071617800650131. Go to original source...
    38. Thompson, J.D.; Higgins, D.G. & Gibson, T.J. (1994): CLUSTALW: Improving the sensitivity of progressive multiple sequence through weighing, position-specific gap penalties and weight matrix choice. - Nucleic Acids Research 22: 4673-4680. doi: 10.1093/nar/22.22.4673. Go to original source...
    39. Treshnikov, A.F. (1985): Atlas of the Arctic. - 204 pp., Main Department of Geodesy and Cartography under the Council of Ministers of the USSR, Moscow.
    40. Tsarenko, P.M. (2011): Chlorococcales. - In: Tsarenko, P.M.; Wasser, S.P. & Nevo, E. (eds): Algae of Ukraine: diversity, nomenclature, taxonomy, ecology and geography, Volume 3, Chlorophyta. - pp. 232-264, A.R.A. Gantner Verlag K.-G, Ruggell.
    41. White, T.J.; Bruns, T.D.; Lee, S.B. & Taylor, J.W. (1990): Amplification and Direct Sequencing of Fungal Ribosomal RNA Genes for Phylogenetics. - In: Innis, M.A.; Gelfand, D.H.; Sninsky, J.J. & White, T.J. (еds): PCR Protocols: A Guide to Methods and Applications. - pp. 315-322, Academic Press, New York. http://dx.doi.org/10.1016/B978-0-12-372180-8.50042-1. Go to original source...
    42. Wolf, M.; Chen, S.; Song, J.; Ankenbrand, M. & Müller, T. (2013): Compensatory Base Changes in ITS2 Secondary Structures Correlate with the Biological Species Concept Despite Intragenomic Variability in ITS2 Sequences - A Proof of Concept. - PLoS ONE 8: e66726. DOI: 10.1371/journal.pone.0066726. Go to original source...
    43. Zuker, M. (2003): Mfold web server for nucleic acid folding and hybridization prediction. - Nucleic Acids Research 31: 3406-3415. DOI: 10.1093/nar/gkg595. Go to original source...

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