Isolation and Characterization of Six Abscisic Acid-Inducible Genes from Carrot Somatic Embryos

Hajime SHIOTA; Guangxiao YANG; Shihua SHEN; Chang-Ho EUN; Ken-ichi WATABE; Ichiro TANAKA; Hiroshi KAMADA

doi:10.5511/plantbiotechnology.21.309

Note

Isolation and Characterization of Six Abscisic Acid-Inducible Genes from Carrot Somatic Embryos

Hajime SHIOTA, Guangxiao YANG, Shihua SHEN, Chang-Ho EUN, Ken-ichi WATABE, Ichiro TANAKA, Hiroshi KAMADA

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Keywords: abscisic acid (ABA), carrot (Daucus carota L), somatic embryogenesis, seed desiccation tolerance

JOURNAL FREE ACCESS

2004 Volume 21 Issue 4 Pages 309-314

DOI https://doi.org/10.5511/plantbiotechnology.21.309

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Abstract

In carrot (Daucus carota L.) somatic embryos, desiccation tolerance is induced by treatment with abscisic acid (ABA). Six cDNA clones that showed ABA-enhanced expression were isolated from carrot by differential screening with ABA-treated and ABA-untreated somatic embryos, and they were named the CAISE (carrot ABA-induced in somatic embryos) genes. Five of the clones encode late embryogenesis abundant (LEA) proteins, and the other clone encodes a glucose and ribitol dehydrogenase. The expression of the CAISE genes was detected in maturing seeds, embryogenic cells, and ABA-treated somatic embryos in which exhibit desiccation tolerance induced by endogenous or exogenous ABA. These results indicate that ABA-induced desiccation tolerance in carrot somatic embryos may be induced by the LEA proteins and the glucose and ribitol dehydrogenase encoded by these ABA-inducible genes.

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