Axenic shoot culture of Anthemis nobilis L. was established from young shoots of plants cultivated in a field. The shoots grew rapidly in hormone-free Murashige-Skoog liquid medium although they proliferated on the solid medium. In the shoots cultured in Murashige-Skoog liquid medium at 25°C, GC-MS analysis revealed that geranyl isovalerate was produced in greater amounts than angelates. In the roots of plantlets obtained from shoot cultures, angelates were not detectable by GC-MS, but higher content of geranyl isovalerate were detected. The plantlets obtained by culturing on hormone-free Murashige-Skoog solid medium at 25°C in 16h/day light were transplanted to pots. At the early cultivation stage, levels of geranyl isovalerate and isobutyl angelate were the same. At the later cultivation stage (20 weeks), isobutyl angelate became the main compound, and the production of geranyl isovalerate decreased gradually.