The Gene Expression Profile of Human Umbilical Vein Endothelial Cells Stimulated by Tumor Necrosis Factor a Using DNA Microarray Analysis

Takeshi Murakami; Chikage Mataki; Chizuru Nagao; Michihisa Umetani; Youichiro Wada; Masami Ishii; Shuichi Tsutsumi; Takahide Kohro; Akio Saiura; Hiroyuki Aburatani; Takao Hamakubo; Tatsuhiko Kodama

doi:10.5551/jat1994.7.39

Takeshi Murakami, Chikage Mataki, Chizuru Nagao, Michihisa Umetani, Youichiro Wada, Masami Ishii, Shuichi Tsutsumi, Takahide Kohro, Akio Saiura, Hiroyuki Aburatani, Takao Hamakubo, Tatsuhiko Kodama

Author information

Takeshi Murakami
Department of Molecular Biology and Medicine
Chikage Mataki
Department of Molecular Biology and Medicine
Chizuru Nagao
Department of Molecular Biology and Medicine
Michihisa Umetani
Department of Molecular Biology and Medicine
Youichiro Wada
Department of Molecular Biology and Medicine
Masami Ishii
Genome Sciences, Research Center for Advanced Science and Technology, The University of Tokyo
Shuichi Tsutsumi
Genome Sciences, Research Center for Advanced Science and Technology, The University of Tokyo
Takahide Kohro
Department of Molecular Biology and Medicine
Akio Saiura
Department of Molecular Biology and Medicine
Hiroyuki Aburatani
Genome Sciences, Research Center for Advanced Science and Technology, The University of Tokyo
Takao Hamakubo
Department of Molecular Biology and Medicine
Tatsuhiko Kodama
Department of Molecular Biology and Medicine

Keywords: DNA microarray analysis, Tumor necrosis factor α (TNFα), Endothelial cells, Transcriptional regulation

JOURNAL FREE ACCESS

2000 Volume 7 Issue 1 Pages 39-44

DOI https://doi.org/10.5551/jat1994.7.39

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Abstract

Stimulation of vascular endothelial cells by tumor necrosis factor α (TNFα) plays a critical role in the pathogenesis of inflammation and vascular diseases. Changes in the gene expression profile in cultured human umbilical vein endothelial cells (HUVEC) treated with TNFα was analyzed with high-density oligonucleotide arrays comprised of 35, 000 genes. TNFα stimulation profoundly induced genes involved in signal transduction, leukocyte adhesion and chemoattraction. ICAM-1 mRNA (fold change 111.9) was most profoundly induced followed by TNFα receptor-associated factor 1 (TRAF1) (95.5), Bcl3 (71.8), IL8 (65. 4), fractalkaine (62.4), E-selectin (48.0), lymphotoxin, β (41.3) and VCAM-1 (31.7). In addition to these previously known genes, 18 poorly characterized or novel genes known as ESTs profoundly induced by TNFα. Initial sequencing analysis identified three of these the genes for squalene epoxydase, chromodomain helicase DNA binding protein 4, and CLP respectively. Further analysis of these genes will provide important information about TNF α signaling and function in vascular endothelial cells.

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