Full Papers
Assessing serum IgG4 glycosylation profiles of IgG4-related disease using lectin microarray
C. Hu1, P. Zhang2, L. Li3, C. Liu4, J. Li5, W. Zhang6, Y. Li7
- Department of Rheumatology, Peking Union Medical College Hospital, Chinese Academy of Medical Science & Peking Union Medical College, Beijing, China.
- Department of Rheumatology, Peking Union Medical College Hospital, Chinese Academy of Medical Science & Peking Union Medical College, Beijing, China.
- Department of Clinical Laboratory, Peking Union Medical College Hospital, Chinese Academy of Medical Science & Peking Union Medical College, Beijing, China.
- Department of Clinical Laboratory, Peking Union Medical College Hospital, Chinese Academy of Medical Science & Peking Union Medical College, Beijing, China.
- Department of Rheumatology, Peking Union Medical College Hospital, Chinese Academy of Medical Science & Peking Union Medical College, Beijing, China.
- Department of Rheumatology, Peking Union Medical College Hospital, Chinese Academy of Medical Science & Peking Union Medical College, Beijing, China.
- Department of Rheumatology and Department of Clinical Laboratory, Peking Union Medical College Hospital, Chinese Academy of Medical Science & Peking Union Medical College, Beijing, China. yongzhelipumch@163.com
CER12902
2021 Vol.39, N°2
PI 0393, PF 0402
Full Papers
PMID: 32662414 [PubMed]
Received: 28/10/2019
Accepted : 05/05/2020
In Press: 30/06/2020
Published: 09/04/2021
Abstract
OBJECTIVES:
IgG4 related disease (IgG4-RD) is a multiorgan fibroinflammatory disorder. Lectin microarray is a high-throughput glycosylation analysis technology. The aim of our study was to investigate glycosylation profiling of serum IgG4 from IgG4-RD patients and controls.
METHODS:
A large cohort of 167 IgG4-RD patients, 130 disease controls (DCs) and 86 healthy controls (HCs) were included in the current study. The glycan level of serum IgG4 of all participants was determined by lectin microarray. A verification assay of lectin microarray and lectin blot were used to clarify the relationship between the serum IgG4 and purified IgG4 glycosylation.
RESULTS:
The results revealed that the glycan level of mannose (binding MNA-M, VVA mannose, ConA) was significantly increased and that the glycan level of fucose (binding LTL), GlcNAc (binding DSL), GalNAc (binding HPA) was significantly decreased in IgG4-RD patients compared to DCs and HCs. We further found that the glycan level of GlcNAc was positively correlated with that of complement 3 (C3), and that the reduced level of GlcNAc was associated with damage to multiple organs. In addition, the mannose level (binding MNA-M and VVA mannose) was negatively correlated with C3 and complement 4 (C4) levels.
CONCLUSIONS:
Serum IgG4 of IgG4-RD patients exhibits different glycosylation levels. This study demonstrated that there is important clinical value in identifying aberrant GlcNAc levels as a potential diagnostic index for multi-organ involvement. Furthermore, the mannose level of serum IgG4 may reflect the degree of inflammation of IgG4-RD.
