Vol 50, No 3 (2012)
Original paper
Published online: 2012-10-08
DGAT2 revealed by the immunogold technique in Arabidopsis thaliana lipid bodies associated with microtubules
DOI: 10.5603/FHC.2012.0058
Folia Histochem Cytobiol 2012;50(3):427-431.
Abstract
The immunogold technique with anti-diacylglycerol acyltransferase 2 (DGAT2) antibody revealed in
A. thaliana embryo and root meristematic cells gold particles manifesting the presence of DGAT2 in ER as well
as in lipid bodies. This being so, lipid synthesis could take place both in ER and in the lipid bodies. The presence
of microtubules around the lipid bodies was evidenced under transmission EM. Detection of tubulin around the
lipid bodies using the immunogold technique with anti-a-tubulin is in agreement with the above observations.
Connection of lipid bodies with microtubules was also detected by us in other plants where they probably participated
in lipid synthesis. A similar phenomenon may take place in A. thaliana.
A. thaliana embryo and root meristematic cells gold particles manifesting the presence of DGAT2 in ER as well
as in lipid bodies. This being so, lipid synthesis could take place both in ER and in the lipid bodies. The presence
of microtubules around the lipid bodies was evidenced under transmission EM. Detection of tubulin around the
lipid bodies using the immunogold technique with anti-a-tubulin is in agreement with the above observations.
Connection of lipid bodies with microtubules was also detected by us in other plants where they probably participated
in lipid synthesis. A similar phenomenon may take place in A. thaliana.
