Clerodane Diterpenes Isolated from Polyalthia longifolia Induce Apoptosis in Human Leukemia HL-60 Cells

Dina Permata Sari; Masayuki Ninomiya; Mai Efdi; Adlis Santoni; Sanusi Ibrahim; Kaori Tanaka; Mamoru Koketsu

doi:10.5650/jos.62.843

Biochemistry and Biotechnology

Clerodane Diterpenes Isolated from Polyalthia longifolia Induce Apoptosis in Human Leukemia HL-60 Cells

Dina Permata Sari, Masayuki Ninomiya, Mai Efdi, Adlis Santoni, Sanusi Ibrahim, Kaori Tanaka, Mamoru Koketsu

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Keywords: Polyalthia longifolia, clerodane diterpene, HL-60 cell, antileukemic activity, apoptosis

JOURNAL FREE ACCESS

2013 Volume 62 Issue 10 Pages 843-848

DOI https://doi.org/10.5650/jos.62.843

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Abstract

Polyalthia is a versatile genus of shrubs and trees found in tropic and sub-tropic regions. In this study, three clerodane diterpenes, kolavenic acid (1), polyalthialdoic acid (2), and 16α-hydroxy-cleroda-3,13(14)Z-dien-15,16-olide (3) isolated from Polyalthia longifolia leaves were evaluated for their apoptotic potential against human leukemia HL-60 cells. Compounds 2 and 3 inhibited cell proliferation with IC₅₀ values of 21.8 and 13.7 μM, respectively. Morphological changes and DNA fragmentation analysis indicated that these diterpenes induce apoptotic cell death in the HL-60 cells. Our results revealed the importance of P. longifolia as a chemopreventive medicinal plant.

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