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PXD006605

PXD006605 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleInsights In The Molecular Regulation Of Monolignol-derived Product Biosynthesis In The Growing Hemp Hypocotyl
DescriptionLignin and lignans are both deriving from the monolignol pathway. Despite the similarity of their building blocks, they fulfil different functions in planta. Lignin strengthens the tissues of the plant, while lignans are involved in plant defence and growth regulation. Their biosyntheses are tuned both spatially and temporally to suit the development of the plant (water conduction, reaction to stresses). It was previously shown that the growing hemp hypocotyl is a valid system to study secondary growth and the molecular events accompanying lignification. The present work confirms the validity of this system, by using it to study the regulation of lignin and lignan biosyntheses. Microscopic observations, lignin analysis, proteomics, together with targeted RT-qPCR and in situ laccase and peroxidase activity assays were carried out to understand the dynamics of lignan/lignin synthesis during the development of the hemp hypocotyl. Based on phylogenetic analysis and targeted gene expression, we suggest a role for the hemp dirigent and dirigent-like proteins. The transdisciplinary approach adopted resulted in the gene- and protein-level quantification of the main enzymes involved in the biosynthesis of monolignols and their oxidative coupling (laccases and class III peroxidases), in lignin deposition (dirigent-like proteins) and in the determination of the stereoconformation of lignans (dirigent proteins). Our work sheds light on how, in the growing hemp hypocotyl, the provision of the precursors needed to synthesize the aromatic biopolymers lignin and lignans is regulated at the transcriptional and proteomic level.
HostingRepositoryPRIDE
AnnounceDate2018-01-08
AnnouncementXMLSubmission_2018-01-08_07:06:01.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD006605
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterkjell sergeant
SpeciesList scientific name: Cannabis; NCBI TaxID: 3482;
ModificationListdeaminated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentTripleTOF 5600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-05-26 04:46:23ID requested
12018-01-08 07:06:02announced
Publication List
Behr M, Sergeant K, Leclercq CC, Planchon S, Guignard C, Lenouvel A, Renaut J, Hausman JF, Lutts S, Guerriero G, Insights into the molecular regulation of monolignol-derived product biosynthesis in the growing hemp hypocotyl. BMC Plant Biol, 18(1):1(2018) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Cannabis sativa, cell wall, lignin, lignan,
Contact List
Jean-François Hausman
contact affiliationLuxemburg Institute of Science and Technology
contact emailjean-francois.hausman@list.lu
lab head
kjell sergeant
contact affiliationLuxembourg Institute of Science and Technology (LIST) Environmental Research and Innovation (ERIN)
contact emailkjell.sergeant@list.lu
dataset submitter
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Dataset FTP location
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