PXD008851

PXD008851 is an original dataset announced via ProteomeXchange.

Title	Analysis of extracelullar vesicles (EVs) protein cargo across the bovine estrous cycle
Description	The success of the early reproductive events depends on an appropriate communication between gametes/embryos and the oviduct. Extracellular vesicles (EVs) contained in oviductal secretions have been suggested as new players in mediating this crucial cross-talk by transferring their cargo (e.g. proteins, mRNA and small ncRNA) from cell to cell. Therefore, the objective of the project was to analyze the protein content of EVs (exosomes and microvesicles) contained in the bovine oviductal fluid at different stages of the estrus cycle. For this purpose, pairs of oviducts with their attached ovaries were collected from cyclic cows at a local abattoir under different hormonal regulation along the estrous cycle. Oviducts were classified into one of the 4 stages of the estrous cycle according to the morphology of the corpus lutea(CL) and follicle populations: Stage 1 (S1): showed recently ovulated follicle (d 1 to 4 of estrous cycle); Stage 2 (S2): early luteal development with medium or large or both present (d 5 to 11); Stage 3 (S3): fully functional CL yellow or orange in color (d 11 to 17); or Stage 4 (S4): regressing CL with little vasculature and a large preovulatory follicle present (d 18 to 20). EVs were collected by ultracentrifugation and prepared for mass spectrometry analysis.
HostingRepository	PRIDE
AnnounceDate	2018-07-31
AnnouncementXML	Submission_2018-08-29_02:38:19.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD008851
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Lucie Combes-Soia
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	Oxidation; Carbamidomethyl
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2018-02-05 01:50:59	ID requested
1	2018-07-31 02:41:14	announced
⏵ 2	2018-08-29 02:38:20	announced	Updated publication reference for PubMed record(s): 30134841.

Almi, ñ, ana C, Tsikis G, Labas V, Uzbekov R, da Silveira JC, Bauersachs S, Mermillod P, Deciphering the oviductal extracellular vesicles content across the estrous cycle: implications for the gametes-oviduct interactions and the environment of the potential embryo. BMC Genomics, 19(1):622(2018) [pubmed]

curator keyword: Biological

submitter keyword: Extracellular vesicles, exosomes, oviduct, estrous cycle, gamete/embryo-maternal interaction, mass spectrometry.

Carmen Alminana Brines
contact affiliation	Institud National de la Recherche Agronomique (INRA), UMR7247 PRC, Nouzilly, France.
contact email	Carmen.Alminana-Brines@inra.fr
lab head
Lucie Combes-Soia
contact affiliation	INRA
contact email	lucie.combes-soia@inra.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/07/PXD008851

PRIDE project URI

• PRIDE
  1. PXD008851
     1. Label: PRIDE project
     2. Name: Analysis of extracelullar vesicles (EVs) protein cargo across the bovine estrous cycle