PXD045450

PXD045450 is an original dataset announced via ProteomeXchange.

Title	Characterization of RSK3 interactome in human cells
Description	Cellular senescence is characterized by a proliferation arrest accompanied by a senescence-associated secretory phenotype (SASP) in normal epithelial cells. During carcinogenesis cells lose the ability to undergo senescence, at least the associated proliferation arrest, in response to stresses such as oncogenes, or TGFβ, a SASP molecule able to mediate autocrine and paracrine senescence. A genetic screen in human mammary epithelial cells (HMECs) identified that the serine threonine kinase RSK3 reverts TGFβ-induced senescence. Interestingly, RSK3 expression decreases in response to TGFβ in a SMAD3-dependent manner, and its constitutive expression rescues SMAD3-induced premature senescence, indicating that decrease of RSK3 itself contributes to TGFβ-induced senescence. Mechanistically, RSK3 regulates senescence by inhibiting NF-κΒ pathway through the decrease in proteasome-mediated IκBα degradation. To better understand how RSK3 might interfere with IκBα degradation, we conducted immunoprecipitation experiments to purify the RSK3-Flag protein and its partners. Mass spectrometry-based proteomics was then performed to identify potential partners.
HostingRepository	PRIDE
AnnounceDate	2023-11-27
AnnouncementXML	Submission_2023-11-27_02:49:50.670.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD045450
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Yohann Couté
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-09-18 04:43:27	ID requested
⏵ 1	2023-11-27 02:49:51	announced

10.1186/S13046-023-02909-5;

10.6019/PXD045450;

submitter keyword: RSK3,Senescence, TGFβ, Epithelial-mesenchymal transition, immunoprecipitation, nanoLC-MS/MS

Yohann Couté
contact affiliation	EDyP
contact email	yohann.coute@cea.fr
lab head
Yohann Couté
contact affiliation	EDyP
contact email	yohann.coute@cea.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/11/PXD045450

PRIDE project URI

• PRIDE
  1. PXD045450
     1. Label: PRIDE project
     2. Name: Characterization of RSK3 interactome in human cells