Vol. 56, 2017
(update: 2017.2.2)
Phylogeographic Identification of Tench Tinca tinca
(L., 1758) (Actinopterygii: Cyprinidae) from the Northern Balkans and
Adjacent Regions and its Implications for Conservation
Jelena Lujić1,*, Klaus Kohlmann2, Petra
Kersten2, Zoran Marinović1,3, Miroslav Ćirković4,
and Vladica Simić5
doi:10.6620/ZS.2017.56-03
1Department of Aquaculture, Szent István
University, 2100 Gödöllő, Hungary. E-mail: zor.marinovic@gmail.com
2Department
of Ecophysiology and Aquaculture, Leibniz-Institute of Freshwater
Ecology and Inland Fisheries, 12587 Berlin, Germany. E-mail:
kohlmann@igb-berlin.de; kersten@igb-berlin.de
3Department of Biology and Ecology, University of Novi
Sad, 21000 Novi Sad, Serbia. E-mail: zor.marinovic@gmail.com
4Scientific Veterinary Institute “Novi Sad”, 21000
Novi Sad, Serbia. E-mail: miroslavcirkovic@yahoo.com
5Institute of Biology and Ecology, University of
Kragujevac, 34000 Kragujevac, Serbia. E-mail: simic@kg.ac.rs
(Received 24 October 2016; Accepted 18 January 2017;
Communicated by Ka Hou Chu)
Jelena Lujić, Klaus Kohlmann, Petra Kersten, Zoran Marinović,
Miroslav Ćirković, and Vladica
Simić (2017) The tench, Tinca
tinca,
is an endangered freshwater fish species in the Balkans. However, there
are no management and conservation strategies developed for this
species so far. In order to be able to develop such strategies, we
first determined the phylogeographic identity of 70 tench individuals
from four countries (Serbia, FYRO Macedonia, Hungary and Croatia) by
PCR-RFLP analyses of two nuclear markers (Act and RpS7)
and one mitochondrial marker (Cytb).
All makers enabled the identification of two major geographic clades of
tench (Western and Eastern), while nuclear markers additionally enabled
the identification of hybrids between the two clades. Based on the
mitochondrial marker Cytb, tench populations can be separated
into two distinct areas: areas north of the Danube River with the
dominant Western origin, and areas south of the Danube River with the
dominant Eastern origin. Data obtained for the Act gene
demonstrated Eastern origin for most individuals (88.23%) while data
obtained for the RpS7
gene demonstrated mixed origin with a high percentage of hybrids. The
presence of high numbers of individuals with Western alleles for the RpS7
gene in areas south of the Danube may indicate a natural invasion of
this phylogroup. According to these results, areas north and south of
the Danube are identified as two main management units. Additionally,
we identified the rare western haplotype W2 based on the Cytb
marker which clearly indicated human-aided dispersals of tench in the
investigated region and since some individuals with W2 origin were
cultured, attention must be given to the genetic structure and identity
of the introduced individuals, whether during introduction or
reintroduction since biological and ecological consequences of the
hybridization between the two major clades are still unknown. Finally,
we propose and discuss management and conservation strategies for tench
of both management areas.
Key words: Tench, Phylogeographic clades,
Conservation, Management strategy, Population genetics.
*Correspondence: Tel: +381646259819. E-mail:
lujicjelena@gmail.com
Citation: Lujić J, Kohlmann K, Kersten P, Marinović Z, Ćirković M, Simić V. 2017. Phylogeographic identification of tench Tinca tinca
(L., 1758) (Actinopterygii: Cyprinidae) from the northern Balkans and
adjacent regions and its implications for conservation. Zool Stud 56:3. doi:10.6620/ZS.2017.56-03.
|