Trichorzins HA and MA, Antibiotic Peptides from Trichoderma harzianum II. Sequence Determination

SANAE HLIMI; SYLVIE REBUFFAT; CHRISTOPHE GOULARD; SYLVIE DUCHAMP; BERNARD BODO

doi:10.7164/antibiotics.48.1254

Trichorzins HA and MA, Antibiotic Peptides from Trichoderma harzianum

II. Sequence Determination

SANAE HLIMI, SYLVIE REBUFFAT, CHRISTOPHE GOULARD, SYLVIE DUCHAMP, BERNARD BODO

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JOURNAL FREE ACCESS

1995 Volume 48 Issue 11 Pages 1254-1261

DOI https://doi.org/10.7164/antibiotics.48.1254

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Abstract

A series of 18-residue antibiotic-antifungal peptides, trichorzins HA and MA, were isolated from Trichoderma harzianum strains exhibiting antagonistic properties against phytopathogenic fungi. The sequences of the nine major pure peptides isolated by HPLC were determined by positive ion FAB-MS data and two-dimensional NMR measurements, including COSY, HOHAHA, ROESY and ¹H-¹³C LRCOSY experiments.

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