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2015 Vol.33, Issue 5 Preview Page
Development of a Single-nucleotide Polymorphism Marker for the Sw-5b Gene Conferring Disease Resistance to Tomato spotted wilt virus in Tomato
Hyung Jin Lee1
,
Boyoung Kim1
,
Chungyun Bae1
,
Won-Hee Kang2
,
Byoung-Cheorl Kang2
,
Inhwa Yeam3
,
Chang-Sik Oh1*
1Department of Horticultural Biotechnology and Institute of Life Science & Resources, College of Life Science, Kyung Hee University
2Department of Plant Science, Seoul National University
3Horticulture and Breeding Major, School of Bioresource Sciences, Andong National University
*교신저자. *Corresponding Author. 
31 October 2015. pp. 730-736
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Abstract

Tomato spotted wilt virus (TSWV) causes one of the most destructive viral diseases that threatens global tomato production. Sw-5b was reported as the resistance gene effective against TSWV. The objective of this research was to develop a single-nucleotide polymorphism (SNP) marker to distinguish tomato cultivars resistant to TSWV from susceptible cultivars for marker-assisted breeding. First, we determined genotypes for TSWV resistance in 32 commercial tomato cultivars using the previously reported Sw-5b gene-based marker. Then, DNA sequences of Sw-5b alleles in tomato cultivars showing resistant or susceptible genotypes were analyzed; a single SNP was found to distinguish tomato cultivars resistant to TSWV from susceptible cultivars. Based on the confirmed SNP, a SNP primer pair was designed. Using this new SNP sequence and high-resolution melting analysis, the same 32 tomato cultivars were screened. The results were perfectly correlated with those from screening with the Sw-5b gene-based marker. These results indicate that the SNP maker developed in this study will be useful for better tracking of resistance to TSWV in tomato breeding.

Keywords
Genotypes
HRM
marker-assisted selection
SNP marker
TSWV

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Information
  • Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
  • Publisher(Ko) :원예과학기술지
  • Journal Title :Horticultural Science and Technology
  • Journal Title(Ko) :원예과학기술지
  • Volume : 33
  • No :5
  • Pages :730-736
  • Received Date : 2015-02-24
  • Revised Date : 2015-06-01
  • Accepted Date : 2015-06-12
  • DOI :https://doi.org/10.7235/hort.2015.15025
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