1. Neuroscience

The Ionotropic Receptors IR21a and IR25a mediate cool sensing in Drosophila

  1. Lina Ni
  2. Mason Klein  Is a corresponding author
  3. Kathryn V Svec
  4. Gonzalo Budelli
  5. Elaine C Chang
  6. Anggie J Ferrer
  7. Richard Benton
  8. Aravinthan DT Samuel  Is a corresponding author
  9. Paul A Garrity  Is a corresponding author
  1. Brandeis University, United States
  2. Harvard University, United States
  3. University of Miami, United States
  4. University of Lausanne, Switzerland
https://doi.org/10.7554/eLife.13254
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Cite this article
  1. Lina Ni
  2. Mason Klein
  3. Kathryn V Svec
  4. Gonzalo Budelli
  5. Elaine C Chang
  6. Anggie J Ferrer
  7. Richard Benton
  8. Aravinthan DT Samuel
  9. Paul A Garrity
(2016)
The Ionotropic Receptors IR21a and IR25a mediate cool sensing in Drosophila
eLife 5:e13254.
https://doi.org/10.7554/eLife.13254
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Abstract

Animals rely on highly sensitive thermoreceptors to seek out optimal temperatures, but the molecular mechanisms of thermosensing are not well understood. The Dorsal Organ Cool Cells (DOCCs) of the Drosophila larva are a set of exceptionally thermosensitive neurons critical for larval cool avoidance. Here, we show that DOCC cool-sensing is mediated by Ionotropic Receptors (IRs), a family of sensory receptors widely studied in invertebrate chemical sensing. We find that two IRs, IR21a and IR25a, are required to mediate DOCC responses to cooling and are required for cool avoidance behavior. Furthermore, we find that ectopic expression of IR21a can confer cool-responsiveness in an Ir25a-dependent manner, suggesting an instructive role for IR21a in thermosensing. Together, these data show that IR family receptors can function together to mediate thermosensation of exquisite sensitivity.

https://doi.org/10.7554/eLife.13254.001

eLife digest

Animals need to be able to sense temperatures for a number of reasons. For example, this ability allows animals to avoid conditions that are either too hot or too cold, and to maintain an optimal body temperature. Most animals detect temperature via nerve cells called thermoreceptors. These sensors are often extremely sensitive and some can even detect changes in temperature of just a few thousandths of a degree per second. However, it is not clear how thermoreceptors detect temperature with such sensitivity, and many of the key molecules involved in this ability are unknown.

In 2015, researchers discovered a class of highly sensitive nerve cells that allow fruit fly larvae to navigate away from unfavorably cool temperatures. Now, Ni, Klein et al. – who include some of the researchers involved in the 2015 work – have determined that these nerves use a combination of two receptors to detect cooling. Unexpectedly, these two receptors – Ionotropic Receptors called IR21a and IR25a – had previously been implicated in the detection of chemicals rather than temperature. IR25a was well-known to combine with other related receptors to detect an array of tastes and smells, while IR21a was thought to act in a similar way but had not been associated with detecting any specific chemicals. These findings demonstrate that the combination of IR21a and IR25a detects temperature instead.

Together, these findings reveal a new molecular mechanism that underlies an animal’s ability to sense temperature. These findings also raise the possibility that other “orphan” Ionotropic Receptors, which have not been shown to detect any specific chemicals, might actually contribute to sensing temperature instead. Further work will explore this possibility and attempt to uncover precisely how IR21a and IR25a work to detect cool temperatures.

https://doi.org/10.7554/eLife.13254.002

Introduction

Temperature is an omnipresent physical variable with a dramatic impact on all aspects of biochemistry and physiology (Sengupta and Garrity, 2013). To cope with the unavoidable spatial and temporal variations in temperature they encounter, animals rely on thermosensory systems of exceptional sensitivity. These systems are used to avoid harmful thermal extremes and to seek out and maintain body temperatures optimal for performance, survival and reproduction (Barbagallo and Garrity, 2015; Flouris, 2011).

Among the most sensitive biological thermoreceptors described to date are the Dorsal Organ Cool Cells (DOCCs), a recently discovered trio of cool-responsive neurons found in each of the two dorsal organs at the anterior of the Drosophila melanogaster larva (Klein et al., 2015). The DOCCs robustly respond to decreases in temperature as small as a few millidegrees C per second (Klein et al., 2015), a thermosensitivity similar to that of the rattlesnake pit organ (Goris, 2011), a structure known for its extraordinary sensitivity. A combination of laser ablation, calcium imaging and cell-specific inhibition studies was used to establish the DOCCs as critical for mediating larval avoidance of temperatures below ~20˚C, with the thermosensitivity of this avoidance behavior paralleling the thermosensitivity of DOCC physiology (Klein et al., 2015). While the DOCCs are exceptionally responsive to temperature, the molecular mechanisms that underlie their thermosensitivity are unknown.

At the molecular level, several classes of transmembrane receptors have been shown to participate in thermosensation in animals. The most extensively studied are the highly thermosensitive members of the Transient Receptor Potential (TRP) family of cation channels (Palkar et al., 2015; Vriens et al., 2014). These TRPs function as temperature-activated ion channels and mediate many aspects of thermosensing from fruit flies to humans (Barbagallo and Garrity, 2015; Palkar et al., 2015; Vriens et al., 2014). In addition to TRPs, other classes of channels contribute to thermosensation in vertebrates, including the thermosensitive calcium-activated chloride channel Anoctamin 1 (Cho et al., 2012) and the two pore domain potassium channel TREK-1 (Alloui et al., 2006). Recent work in Drosophila has demonstrated that sensory receptors normally associated with other modalities, such as chemical sensing, can also make important contributions to thermotransduction. In particular, GR28B(D), a member of the invertebrate gustatory receptor (GR) family, was shown to function as a warmth receptor to mediate warmth avoidance in adult flies exposed to a steep thermal gradient (Ni et al., 2013). The photoreceptor Rhodopsin has also been reported to contribute to temperature responses, although its role in thermosensory neurons is unexamined (Shen et al., 2011).

Ionotropic Receptors (IRs) are a family of invertebrate receptors that have been widely studied in insect chemosensation, frequently serving as receptors for diverse acids and amines (Benton et al., 2009; Silbering et al., 2011). The IRs belong to the ionotropic glutamate receptor (iGluR) family, an evolutionarily conserved family of heterotetrameric cation channels that includes critical regulators of synaptic transmission like the NMDA and AMPA receptors (Croset et al., 2010). In contrast to iGluRs, IRs have been found only in Protostomia and are implicated in sensory transduction rather than synaptic transmission (Rytz et al., 2013). In insects, the IR family has undergone significant expansion and diversification, with the fruit fly D. melanogaster genome encoding 66 IRs (Croset et al., 2010). While the detailed structures of IR complexes are unknown, at least some IRs are thought to form heteromeric channels in which a broadly-expressed IR 'co-receptor' (such as IR25a, IR8a or IR76b) partners one or more selectively-expressed 'stimulus-specific' IRs (Abuin et al., 2011).

Among insect IRs, IR25a is the most highly conserved across species (Croset et al., 2010). In Drosophila, IR25a expression has been observed in multiple classes of chemosensory neurons with diverse chemical specificities, and IR25a has been shown to function as a 'co-receptor' that forms chemoreceptors of diverse specificities in combination with other, stimulus-specific IRs (Abuin et al., 2011; Rytz et al., 2013). IR21a is conserved in mosquitoes and other insects, but has not been associated with a specific chemoreceptor function (Silbering et al., 2011), raising the possibility that it may contribute to other sensory modalities.

Here, we show that the previously 'orphan' IR, Ir21a, acts together with the co-receptor IR25a to mediate thermotransduction. We show that these receptors are required for larval cool avoidance behavior as well as the physiological responsiveness of the DOCC thermosensory neurons to cooling. Furthermore, we find that ectopic expression of IR21a can confer cool responsiveness in an Ir25a-dependent manner, indicating that IR21a can influence thermotransduction in an instructive fashion.

Results

Dorsal organ cool cells express Ir21a-Gal4

To identify potential regulators of DOCC thermosensitivity, we sought sensory receptors specifically expressed in the dorsal organ housing these thermoreceptors (Figure 1a). Examining a range of potential sensory receptors in the larva, we found that regulatory sequences from the Ionotropic Receptor Ir21a drove robust gene expression (via the Gal4/UAS system [Brand and Perrimon, 1993]) in a subset of neurons in the dorsal organ ganglion, as well as in other locations (Figure 1b,c, Figure 1—figure supplement 1). Within each dorsal organ ganglion, Ir21a-Gal4 drove gene expression in three neurons (Figure 1b,c). These neurons exhibited the characteristic morphology of the DOCCs, which have unusual sensory processes that form a characteristic 'dendritic bulb' inside the larva (Klein et al., 2015).

Figure 1 with 2 supplements see all
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Dorsal Organ Cool Cells (DOCCs) express Ir21a-Gal4.

(a) First/second instar larval anterior. Each Dorsal Organ Ganglion (grey) contains three DOCCs (blue). Anterior-Posterior axis denoted by double-headed arrow. (b,c) Ir21a-Gal4;UAS-GFP (Ir21a>GFP) labels larval DOCCs. Arrows denote cell bodies and arrowheads dendritic bulbs. (d) Temperature responses of Ir21a-Gal4;UAS-GCaMP6m-labeled DOCCs. Left panels, raw images; right panels, colors reflect fluorescence intensity. Arrows denote cell bodies. (e) Fluorescence quantified as percent change in fluorescence intensity compared to minimum intensity. n=22 cells (from 6 animals). (f,g) Temperature-responses of Ir21a-Gal4;R11F02-Gal4;UAS-GCaMP6m-labeled DOCCs. n=26 (7). Scale bars, 10 microns. Traces, average +/- SEM. Figure 1—figure supplement 1 provides an example of the 3-D imaging stacks used for calcium imaging data acquisition.

https://doi.org/10.7554/eLife.13254.003

To confirm that the Ir21a-Gal4-positive neurons were indeed cool-responsive, their thermosensitivity was tested by cell-specific expression of the genetically encoded calcium indicator GCaMP6m under Ir21a-Gal4 control. Consistent with previously characterized DOCC responses (Klein et al., 2015), when exposed to a sinusoidal temperature stimulus between ~14˚C and ~20˚C, GCaMP6m fluorescence in these neurons increased upon cooling and decreased upon warming (Figure 1d,e and Figure 1—figure supplement 2). The expression of Ir21a-Gal4 was also compared with that of R11F02-Gal4 (Figure 1—figure supplement 1), a promoter used in the initial characterization of the DOCCs (Klein et al., 2015). As expected, GCaMP6m expressed under the combined control of Ir21a-Gal4 and R11F02-Gal4 revealed their precise overlap in three cool-responsive neurons with DOCC morphology in the dorsal organ, further confirming the identification of the Ir21a-Gal4-expressing cells as the cool-responsive DOCCs (Figure 1f,g).

Ir21a mediates larval thermotaxis

To assess the potential importance of Ir21a in larval thermosensation, we tested the ability of animals to thermotax when Ir21a function has been eliminated. Two Ir21a alleles were generated, Ir21a123 and Ir21a∆1. Ir21a123 deletes 23 nucleotides in the region encoding the first transmembrane domain of IR21a and creates a translational frameshift (Figure 2a). Ir21a∆1 is an ~11 kb deletion removing all except the last 192 nucleotides of the Ir21a open reading frame, including all transmembrane and ion pore sequences (Figure 2a). As the deletion in Ir21a∆1 could also disrupt the nearby chitin deacetylase 5 (cda5) gene (Figure 2—figure supplement 1), Ir21a-specific rescue experiments were performed to confirm all defects reflected the loss of Ir21a activity (see below).

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Larval cool avoidance requires Ir21a and Ir25a.

(a) Sequence alterations in Ir21a and Ir25a alleles. Ir21a regulatory sequences present in Ir21a-Gal4 are denoted in green and regions encoding transmembrane domains (TMs) and pore region in red. Additional details provided in Figure 2—figure supplement 1. (b) Thermotaxis is quantified as navigational bias. Cool avoidance behavior was assessed by tracking larval trajectories on a ~0.36˚C/cm gradient extending from ~13.5˚C to ~21.5˚C, with a midpoint of ~17.5˚C. (c) Cool avoidance requires Ir21a and Ir25a. Ir21a>Ir21a denotes a wild type Ir21a transcript expressed under Ir21a-Gal4 control. {Ir21a+} and {Ir25a+} denote wild type genomic rescue transgenes. Letters denote statistically distinct categories (alpha=0.05; Tukey HSD). wild type, n=836 animals. Ir21a∆1, n=74. Ir21a∆1;Ir21a-Gal4, n=48. Ir21a∆1;UAS-Ir21a, n=10. Ir21a∆1;Ir21a>Ir21a, n= 88. Ir21a∆1/ Ir21a123, n=71; Ir21a∆1/ Ir21a123; {Ir21a+} n=70; Ir25a2, n =100. Ir25a2; {Ir25a+} n= 247. Additional mutant analyses provided in Figure 2—figure supplement 1.

https://doi.org/10.7554/eLife.13254.006

The loss of Ir21a function strongly disrupted larval thermotaxis. When exposed to a thermal gradient of ~0.36˚C/cm, ranging from ~13.5˚C to ~21.5˚C, Ir21a∆1 null mutants as well as Ir21a123/ Ir21a∆1 heterozygotes were unable to navigate away from cooler temperatures and toward warmer temperatures (Figure 2b,c). These defects could be rescued by expression of a wild-type Ir21a transcript under Ir21a-Gal4 control and by a wild-type Ir21a genomic transgene (Figure 2c). Taken together, these results are consistent with a critical role for Ir21a in larval thermotaxis.

Ir25a mediates larval thermotaxis and is expressed in DOCCs

As IRs commonly act in conjunction with 'co-receptor' IRs, we examined the possibility that larval thermotaxis involved such additional IRs. Animals homozygous for loss-of-function mutations in two previously reported IR co-receptors, Ir8a and Ir76b, exhibited robust avoidance of cool temperatures, indicating that these receptors are not essential for this behavior (Figure 2—figure supplement 1). By contrast, Ir25a2 null mutants failed to avoid cool temperatures, a defect that could be rescued by the introduction of a transgene containing a wild type copy of Ir25a (Figure 2c). Thus, Ir25a also participates in cool avoidance. To assess IR25a expression, larvae were stained with antisera for IR25a. Robust IR25a protein expression was detected in multiple cells in the dorsal organ ganglion, including the three Ir21a-Gal4-expressing DOCCs (Figure 3a). Within DOCCs, IR25a strongly labels the 'dendritic bulbs', consistent with a role in sensory transduction. Staining was absent in Ir25a null mutants demonstrating staining specificity (Figure 3b). Thus Ir25a is required for thermotaxis and is expressed in the neurons that drive this behavior.

Figure 3
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DOCCs express IR25a.

(a) Left panel, Ir21a>GFP-labeled DOCCs. Middle panel, IR25a protein expression in dorsal organ. Right panel, Ir21a>GFP-labeled DOCCs express IR25a protein. Arrows denote DOCC cell bodies and arrowheads DOCC dendritic bulbs. (b) IR25a immunostaining is not detected in Ir25a2 null mutants. Scale bar, 10 microns.

https://doi.org/10.7554/eLife.13254.008

Ir21a and Ir25a are required for cool detection by DOCCs

To assess whether Ir21a and Ir25a contribute to cool detection by the DOCCs, DOCC cool-responsiveness was examined using the genetically encoded calcium sensor GCaMP6m. Consistent with a role for Ir21a in cool responses, DOCCs exhibited strongly reduced responses to cooling in Ir21a∆1 deletion mutants, and this defect was robustly rescued by expression of an Ir21a transcript in the DOCCs using R11F02-Gal4 (Figure 4a-e,h). Similarly, DOCC thermosensory responses were greatly reduced in Ir25a mutants, a defect that was rescued by a wild type Ir25a transgene (Figure 4f–h). Together these data demonstrate a critical role for Ir21a and Ir25a in the detection of cooling by the DOCCs.

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DOCC cool responses require Ir21a and Ir25a.

DOCC responses monitored using R11F02>GCaMP6m. DOCCs exhibit robust cool-responsive increases in fluorescence (a,c), which are dramatically reduced in Ir21a (b,d) and Ir25a (f) mutants. (e) Ir21a transcript expression under R11F02-Gal4 control rescues the Ir21a mutant defect. (g) Introduction of an Ir25a genomic rescue transgene rescues the Ir25a mutant defect. (h) Ratio of fluorescence at 14˚C versus 20˚C depicted using a violin plot. Letters denote statistically distinct categories, p<0.0001, Steel-Dwass test. Scale bars, 10 microns. Traces, average +/- SEM. wild type, n=33 cells (from 11 animals). Ir21a∆1, n= 58 (14). Ir21a∆1; R11F02>Ir21a, n=32 (9). Ir25a2, n=43 (16). Ir25a2; {Ir25a+}, n=30 (10). Analyses of brv1 and brv2 mutants provided in Figure 4—figure supplement 1.

https://doi.org/10.7554/eLife.13254.009

Prior work has suggested that three TRP channels, Brivido-1, Brivido-2 and Brivido-3, work together to mediate cool sensing in adult thermosensors (Gallio et al., 2011). Putative null mutations are available for two of these genes, brv1 and brv2, and we used these alleles to test the potential role of Brivido function in DOCC cool sensing (Gallio et al., 2011). Although brv1 mutant showed defects in thermotactic behavior, DOCC responses to cooling appeared unaffected in brv1 mutants (Figure 4—figure supplement 1). brv2 nulls exhibited no detectable thermotaxis defects (Figure 4—figure supplement 1). Thus, we detect no role for these receptors in cool sensing by the DOCCs.

Ectopic IR21a expression confers cool-sensitivity in an Ir25a-dependent manner

The requirement for Ir21a and Ir25a in DOCC-mediated cool sensing raised the question of whether ectopic expression of these receptors could confer cool-responsiveness upon a cell, as might be predicted for a cool receptor. Attempts to express IR21a and IR25a together or separately in heterologous cells, including S2 cells, Xenopus oocytes and HEK cells, failed to yield detectable responses to cooling or warming, as did attempts to confer thermosensitivity upon non-thermosensitive neurons by ectopically expressing them separately or together in Drosophila, broadly throughout the larval nervous system and in adult chemosensory neurons (G.B., L.N., M.K. and P.G, unpublished). However, ectopic expression of IR21a in one set of neurons in the adult, Hot Cell thermoreceptors in the arista that normally respond to warming rather than cooling, conferred cool-sensitivity.

The adult arista contains three warmth-activated thermosensory neurons, termed Hot Cells (or HC neurons) (Gallio et al., 2011). We found that forced expression of IR21a in the HC neurons could significantly alter their response to temperature. As previously reported (Gallio et al., 2011), wild-type HC neurons respond to warming with robust increases in intracellular calcium and to cooling with decreases in intracellular calcium, as reflected in temperature-dependent changes in GCaMP6m fluorescence (Figure 5a,c). In contrast, HC neurons in which IR21a is expressed under the control of a pan-neuronal promoter (N-syb>Ir21a animals) frequently exhibited elevations in calcium not only in response to warming, but also at the coolest temperatures (Figure 5b,d,f, Figure 5—figure supplement 1a). Thus, ectopic IR21a expression causes HC neurons, which are normally inhibited by cooling, to become responsive to both cooling and warming.

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IR21a expression confers cool-sensitivity upon warmth-responsive Hot Cell neurons.

(a,b) Temperature responses of wild type (a) or N-syb>Ir21a-expressing (b) thermoreceptors in the arista, monitored with N-syb>GCaMP6m. Cell bodies of warmth-responsive Hot Cells outlined in red and cool-responsive Cold Cells in blue. Arrows highlight Hot Cells at 14˚C. Traces of Hot Cell and Cold Cell responses shown at right. Scale bar, 10 microns. (c-e) Fluorescence of Hot Cells in response to sinusoidal 14˚C to 30˚C temperature stimulus, quantified as percent ∆F/Fmin. Dotted lines denote temperature minima. Traces, average +/- SEM. (f) Difference between ∆F/Fmin at 14˚C vs 20˚C (average +/- SEM). Responses of N-syb>Ir21a cells were statistically distinct from both wild type and Ir25a2;N-syb>Ir21a (p<0.01, Steel-Dwass test; letters denote statistically distinct groups). wild type, n= 16 cells (from 8 animals). N-syb>Ir21a, n= 16 (10). Ir25a2; N-syb>Ir21a, n= 20 (10). Analysis of endogenous IR25a expression in the Hot Cells and of the consequences of Hot Cell-specific misexpression of IR21a provided in Figure 5—figure supplement 1.

https://doi.org/10.7554/eLife.13254.011

As Ir21a-dependent cool detection in the DOCCs relies upon Ir25a, we examined the requirement for Ir25a in IR21a-mediated cool activation of the HC neurons. Consistent with previously reported IR25a expression in the arista (Benton et al., 2009), we observed robust IR25a protein expression in the HC neurons (Figure 5—figure supplement 1b,c). Consistent with a role for Ir25a in Ir21a-mediated cool-responsiveness, ectopic IR21a expression failed to drive significant HC neuron cool responses in Ir25a mutants (Figure 5e,f). Thus, IR21a can confer cool-sensitivity upon an otherwise warmth-responsive neuron in an Ir25a-dependent fashion. Similar cool sensitivity was observed when IR21a was ectopically expressed under the control of an HC-specific promoter (HC>Ir21a, Figure 5—figure supplement 1d,e). Finally, ectopic expression of IR21a in Gr28b mutant HC neurons, which lack the Gr28b(D) warmth receptor, yields neurons that respond only to cooling (Figure 6). Together, these data demonstrate that ectopic IR21a expression can confer cool-sensitivity in an Ir25a-dependent fashion.

Figure 6
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Hot Cell-specific expression of IR21a confers cool-sensitivity upon Gr28b mutant Hot Cell neurons.

(a-c) Temperature responses of wild type (a), Gr28b mutant (b), and HC>Ir21a-expressing Gr28b mutant (c) thermoreceptors in the adult arista, monitored using HC>GCaMP6m. Dotted lines denote temperature minima. Traces, mean +/- SEM. wild type, n=11 cells (3 animals). Gr28bMi n=9 (3). HC>IR21a; Gr28bMi n=11 (3). (d) Cool responses (∆F/F14˚C - ∆F/F30˚C) of HC>IR21a; Gr28bMicells were distinct from both wild type and Gr28bMi (p<0.01, Steel-Dwass test, letters denote statistically distinct groups).

https://doi.org/10.7554/eLife.13254.013

Discussion

These data demonstrate that the ionotropic receptors IR21a and IR25a have critical roles in thermosensation in Drosophila, mediating cool detection by the larval dorsal organ cool cells (DOCCs) and the avoidance of cool temperatures. Combinations of IRs have been previously found to contribute to a wide range of chemosensory responses, including the detection of acids and amines (Rytz et al., 2013). These findings extend the range of sensory stimuli mediated by these receptor combinations to cool temperatures. Interestingly, IR21a- and IR25a-dependent cool sensation appears independent of Brivido 1 and Brivido 2, two TRP channels implicated in cool sensing in the adult (Gallio et al., 2011).

The precise nature of the molecular complexes that IRs form is not well understood. IR25a has been shown to act with other IRs in the formation of chemoreceptors, potentially as heteromers (Rytz et al., 2013). This precedent raises the appealing possibility that IR25a might form heteromeric thermoreceptors in combination with IR21a. However, our inability to readily reconstitute temperature-responsive receptor complexes in heterologous cells suggests that the mechanism by which these receptors contribute to cool responsiveness is likely to involve additional molecular cofactors. It is interesting to note that the range of cell types in which ectopic IR21a expression confers cool-sensitivity is so far restricted to neurons that already respond to temperature. This observation suggests the existence of additional co-factors or structures in these thermosensory cells that are critical for IR21a and IR25a to mediate responses to temperature. All studies to date implicate IRs as receptors for sensory stimuli (Rytz et al., 2013), and our misexpression studies are consistent with a similar role for Ir21a and IR25a in cool sensation. However, we cannot formally exclude the possibility that they could have indirect, and possibly separate, functions in this process, for example, in regulating the expression or function of an unidentified cool receptor. Interestingly, IR25a was recently implicated in warmth-responsive resetting of the circadian clock, and suggested to confer warmth-sensitivity on its own, without the co-expression of other IRs (Chen et al., 2015). The ability of IR25a to serve as a warmth receptor on its own would be a surprise given both its broad expression and its established role as an IR co-receptor (Abuin et al., 2011). As IR25a misexpression only slightly enhanced the thermosensitivity of an already warmth-responsive neuron (Chen et al., 2015), this raises the alternative possibility that – analogous to cool-sensing – IR25a acts not on its own, but rather as a co-receptor with other IRs involved in warmth-sensing.

While the present study focuses on the role of IR21a and IR25a in larval thermosensation, it is interesting to note that the expression of both IR21a and IR25a has been detected in the thermoreceptors of the adult arista (Benton et al., 2009). Thus, related mechanisms could contribute to thermosensory responses not only in the DOCCs, but also in other cellular contexts and life stages. Moreover, the presence of orthologs of IR21a and IR25a across a range of insects (Croset et al., 2010) raises the possibility that these IRs, along other members of the IR family, constitute a family of deeply-conserved thermosensors.

Materials and methods

Fly strains

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Ir25a2 (Benton et al., 2009>), BAC{Ir25a+} (Chen et al., 2015), Ir8a1 (Abuin et al., 2011), Ir76b1 (Zhang et al., 2013), Ir76b2 (Zhang et al., 2013), R11F02-Gal4 (Klein et al., 2015), brv1L653stop (Gallio et al., 2011), brv2w205stop (Gallio et al., 2011), HC-Gal4 (Gallio et al., 2011), Gr28bMi (Ni et al., 2013), UAS-GCaMP6m (P{20XUAS-IVS-GCaMP6m}attp2 and P{20XUAS-IVS-GCaMP6m}attp2attP40 [Chen et al., 2013]), UAS-GFP (p{10X UAS-IVS-Syn21-GFP-p10}attP2 [Pfeiffer et al., 2012]), nSyb-Gal4 (P{GMR57c10-Gal4}attP2, [Pfeiffer et al., 2012]), and y1 P(act5c-cas9, w+) M(3xP3-RFP.attP)ZH-2A w* (Port et al., 2014) were previously described.

In Ir21a-Gal4, sequences from -606 to +978 with respect to the Ir21a translational start site (chromosome 2L: 24,173 – 25757, reverse complement) lie upstream of Gal4 protein-coding sequences. UAS-Ir21a contains the Ir21a primary transcript including introns (chromosome 2L: 21823–25155, reverse complement) placed under UAS control. The {Ir21a+} genomic rescue construct contains sequences from -1002 to +4439 with respect to the Ir21a translational start site (chromosome 2L: 26153–20712).

Ir21a∆1 was generated by FLP-mediated recombination between two FRT-containing transposon insertions (PBac{PB}c02720 and PBac{PB}c04017) as described (Parks et al., 2004). Ir21a123 was generated by transgene-based CRISPR-mediated genome engineering as described (Port et al., 2014), with an Ir21a-targeting gRNA (5’-CTGATTTGCGTTTACCTCGG) expressed under U6-3 promoter control (dU6-3:gRNA) in the presence of act-cas9 (Port et al., 2014).

Behaviour

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Thermotaxis of early 2nd instar larvae was assessed over a 15 min period on a temperature gradient extending from 13.5 to 21.5°C over 22 cm (~0.36˚C/cm) as described (Klein et al., 2015). As behavioral data appear normally distributed (as assessed by Shapiro-Wilk test), statistical comparisons were performed by Tukey HSD test, which corrects for multiple comparisons.

Calcium imaging

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Calcium imaging was performed as previously described for larvae (Klein et al., 2015). Pseudocolor images were created using the 16_colors lookup table in ImageJ 1.43r. Adult calcium imaging was performed as described for larvae (Klein et al., 2015), with modifications to the temperature stimulus and sample preparation approach. Adult temperature stimulus ranged from 14°C to 30°C. Intact adult antennae with aristae attached were dissected and placed in fly saline (110 mM NaCl, 5.4 mM KCl, 1.9 mM CaCl2, 20 mM NaHCO3, 15 mM tris(hydroxymethyl)aminomethane (Tris), 13.9 mM glucose, 73.7 mM sucrose, and 23 mM fructose, pH 7.2, [Brotz and Borst, 1996]) on a large cover slip (24 mm x 50 mm) and then covered by a small cover slip (18 mm x 18 mm). The large cover slip was placed on top of a drop of glycerol on the temperature control stage. As quantified calcium imaging data (Figure 4h, Figure 5f, Figure 6d) did not conform to a normal distribution as assessed by Shapiro-Wilk test (p<0.01), statistical comparisons were performed by Steel-Dwass test, a non-parametric test that corrects for multiple comparisons, using JMP11 (SAS).

Immunohistochemistry

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Immunostaining was performed as described (Kang et al., 2012) using rabbit anti-Ir25a (1:100; [Benton et al., 2009]), mouse anti-GFP (1:200; Roche), goat anti-rabbit Cy3 (1:100; Jackson ImmunoResearch), donkey anti-mouse FITC (1:100; Jackson ImmunoResearch).

References

    1. Brand AH
    2. Perrimon N
    (1993)
    Targeted gene expression as a means of altering cell fates and generating dominant phenotypes
    Development 118:401–415.
    1. Brotz TM
    2. Borst A
    (1996)
    Cholinergic and GABAergic receptors on fly tangential cells and their role in visual motion detection
    Journal of Neurophysiology 76:1786–1799.
    1. Pfeiffer BD
    2. Truman JW
    3. Rubin GM
    (2012) Using translational enhancers to increase transgene expression in Drosophila
    Proceedings of the National Academy of Sciences of the United States of America 109:6626–6631.
    https://doi.org/10.1073/pnas.1204520109

Article and author information

Author details

  1. Lina Ni

    1. National Center for Behavioral Genomics, Brandeis University, Waltham, United States
    2. Volen Center for Complex Systems, Brandeis University, Waltham, United States
    3. Department of Biology, Brandeis University, Waltham, United States
    Contribution
    LN, Performed molecular genetics, behavior, immunohistochemistry and calcium imaging, Conception and design, Acquisition of data, Analysis and interpretation of data, Drafting or revising the article, Contributed unpublished essential data or reagents
    Contributed equally with
    Mason Klein
    Competing interests
    The authors declare that no competing interests exist.

  2. Mason Klein

    1. Department of Physics, Harvard University, Cambridge, United States
    2. Department of Physics, University of Miami, Coral Gables, United States
    3. Center for Brain Science, Harvard University, Cambridge, United States
    Contribution
    MK, Performed behavior and calcium imaging, Performed data analysis, Conception and design, Acquisition of data, Analysis and interpretation of data, Drafting or revising the article, Contributed unpublished essential data or reagents
    Contributed equally with
    Lina Ni
    For correspondence
    klein@miami.edu
    Competing interests
    The authors declare that no competing interests exist.

  3. Kathryn V Svec

    1. National Center for Behavioral Genomics, Brandeis University, Waltham, United States
    2. Volen Center for Complex Systems, Brandeis University, Waltham, United States
    3. Department of Biology, Brandeis University, Waltham, United States
    Contribution
    KVS, Performed molecular genetics, Acquisition of data, Contributed unpublished essential data or reagents
    Competing interests
    The authors declare that no competing interests exist.

  4. Gonzalo Budelli

    1. National Center for Behavioral Genomics, Brandeis University, Waltham, United States
    2. Volen Center for Complex Systems, Brandeis University, Waltham, United States
    3. Department of Biology, Brandeis University, Waltham, United States
    Contribution
    GB, Performed physiology, Conception and design, Acquisition of data, Analysis and interpretation of data, Contributed unpublished essential data or reagents
    Competing interests
    The authors declare that no competing interests exist.

  5. Elaine C Chang

    1. National Center for Behavioral Genomics, Brandeis University, Waltham, United States
    2. Volen Center for Complex Systems, Brandeis University, Waltham, United States
    3. Department of Biology, Brandeis University, Waltham, United States
    Contribution
    ECC, Performed molecular genetics, Acquisition of data, Contributed unpublished essential data or reagents
    Competing interests
    The authors declare that no competing interests exist.

  6. Anggie J Ferrer

    Department of Physics, University of Miami, Coral Gables, United States
    Contribution
    AJF, Performed data analysis, Analysis and interpretation of data
    Competing interests
    The authors declare that no competing interests exist.

  7. Richard Benton

    Center for Integrative Genomics, Faculty of Biology and Medicine, University of Lausanne, Lausanne, Switzerland
    Contribution
    RB, Conception and design, Analysis and interpretation of data, Drafting or revising the article, Contributed unpublished essential data or reagents
    Competing interests
    The authors declare that no competing interests exist.

  8. Aravinthan DT Samuel

    1. Department of Physics, Harvard University, Cambridge, United States
    2. Center for Brain Science, Harvard University, Cambridge, United States
    Contribution
    ADTS, Conception and design, Analysis and interpretation of data, Drafting or revising the article, Contributed unpublished essential data or reagents
    For correspondence
    samuel@physics.harvard.edu
    Competing interests
    The authors declare that no competing interests exist.

  9. Paul A Garrity

    1. National Center for Behavioral Genomics, Brandeis University, Waltham, United States
    2. Volen Center for Complex Systems, Brandeis University, Waltham, United States
    3. Department of Biology, Brandeis University, Waltham, United States
    Contribution
    PAG, Conception and design, Acquisition of data, Analysis and interpretation of data, Drafting or revising the article, Contributed unpublished essential data or reagents
    For correspondence
    pgarrity@brandeis.edu
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-8274-6564

Funding

National Institute of Neurological Disorders and Stroke (F32 NS077835)

  • Mason Klein

National Institute of General Medical Sciences (F32 GM113318)

  • Gonzalo Budelli

European Research Council (205202)

  • Richard Benton

European Research Council (615094)

  • Richard Benton

National Institute of General Medical Sciences (P01 GM103770)

  • Aravinthan DT Samuel
  • Paul A Garrity

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Acknowledgements

We thank Rachelle Gaudet and Linda Huang for comments on the manuscript, Guangwei Si for assistance with calcium imaging, Peter Bronk for advice on physiology, Adam Kaplan for creating Ir21a-Gal4, and the Bloomington Stock Center for fly strains. Supported by a grant from the National Institute of Neurological Disorders and Stroke (F32 NS077835) to MK, the National Institute of General Medical Science (F32 GM113318) to GB, European Research Council Starting Independent Researcher and Consolidator Grants (205202 and 615094) to RB, and the National Institute of General Medical Sciences (P01 GM103770) to ADTS and PAG.

Ethics

Animal experimentation: This study (specifically the harvest of Xenopus laevis oocytes) was performed in strict accordance with approved institutional animal care and use committee (IACUC) protocol (#14077) of Brandeis University.

Version history

  1. Received: November 22, 2015
  2. Accepted: March 18, 2016
  3. Version of Record published: April 29, 2016 (version 1)
  4. Version of Record updated: May 3, 2016 (version 2)

Copyright

© 2016, Ni et al.

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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  1. Lina Ni
  2. Mason Klein
  3. Kathryn V Svec
  4. Gonzalo Budelli
  5. Elaine C Chang
  6. Anggie J Ferrer
  7. Richard Benton
  8. Aravinthan DT Samuel
  9. Paul A Garrity
(2016)
The Ionotropic Receptors IR21a and IR25a mediate cool sensing in Drosophila
eLife 5:e13254.
https://doi.org/10.7554/eLife.13254

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https://doi.org/10.7554/eLife.13254

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