Neuropeptides are evolutionarily ancient hormones—secreted, diffusible signaling-molecules—that are expressed in many cells of the nervous and neuroendocrine systems of Bilateria (e.g. vertebrates, insects and worms) and its sister group Cnidaria (e.g. jellyfishes, corals and sea anemones) (Grimmelikhuijzen and Hauser, 2012; Grimmelikhuijzen et al., 2002; Hartenstein, 2006; Walker et al., 2009). They are short polypeptides generated from larger precursor proteins through proteolytic cleavage, and are stored in membrane-bound secretory vesicles that are distributed throughout the neuron. For some, peptide α-amidation occurs via a post-translational, enzymatic conversion of a C-terminal glycine into an amide group, and is often critical for biological activity (reviewed in [Eipper et al., 1992]). Intercellular signaling occurs when neuropeptides are released from the secretory vesicles into the extracellular space and received by receptors of target cells. Neuropeptides have been implicated in regulating a wide range of biological processes—from behavior, physiology, and development—in animals with nervous systems (summarized in [Strand, 1999]). Given that neuropeptides were early neurotransmitters in the evolution of nervous systems, testing hypotheses about the conserved roles of neuropeptides in extant animals is directly relevant to understanding how the primitive nervous system may have functioned.

Comparative genomic evidence suggests that several peptide hormones are shared across Bilateria and Cnidaria, namely an insulin-related peptide, glycoprotein hormone, prokineticin, and amidated peptides with carboxy-terminal sequences Wamide, RFamide and RYamide (Jékely, 2013). Among them, only amidated peptides have been shown to occur in neurons of both bilaterians and cnidarians (e.g. (Conzelmann and Jékely, 2012; Conzelmann et al., 2013; Grimmelikhuijzen, 1985; Koizumi et al., 2004; Schmich et al., 1998a); see also (Walker et al., 2009) for a review of literature on RFamide), and thus are here considered bona fide conserved neuropeptides. The precursor protein of these neuropeptides typically encodes multiple copies of immature neuropeptides (e.g. (Conzelmann and Jékely, 2012; Darmer et al., 1991; Leviev and Grimmelikhuijzen, 1995), some of which can differ in amino acid sequence; this indicates that an immature neuropeptide sequence can duplicate and diverge within a gene. Paralogous neuropeptides can thus be generated from a single gene, or from different genes that emerged by gene duplication (i.e. paralogous genes). Neuropeptides can also be lost during evolution; for example, deuterostome bilaterians (e.g. chordates and echinoderms) lack Wamide precursor orthologs (Conzelmann et al., 2013), and the RYamide-encoding gene appears absent from the genome of anthozoan cnidarians (sea anemones and corals; e.g. (Anctil, 2009).

The ancestral function of the conserved neuropeptides is enigmatic, but a few lines of evidence suggest that Wamide may have conserved roles in metamorphosis regulation across Bilateria and Cnidaria (Conzelmann et al., 2013; Schoofs and Beets, 2013). First, Wamide is expressed in larval nervous systems in bilaterians (e.g. (Conzelmann et al., 2013; Hua et al., 1999) and cnidarians (e.g. [Leitz and Lay, 1995]). Second, exogenous Wamides have effects on molecular, behavioral and/or morphogenetic processes underlying life cycle transition across animals. For instance, Wamide neuropeptides (also known as allatostatin-B, prothoracicostatic peptides, myoinhibitory peptides (MIP), and GLWamide) can inhibit the synthesis of juvenile hormone in crickets (Lorenz et al., 1995) and that of ecdysteroids (the molting hormone) in a silkworm (Hua et al., 1999). Also, they can induce larval settlement of trochophore larvae in annelids (Conzelmann et al., 2013), and metamorphosis of planula larvae into polyps in hydrozoan and scleractinian (hard coral) cnidarians (Erwin and Szmant, 2010; Iwao et al., 2002; Leitz et al., 1994; Schmich et al., 1998b). Third, Wamide signaling appears to be necessary for life cycle transition across Bilateria and Cnidaria. The evidence comes from the annelid bilaterian Platynereis dumerilii, where morpholino-mediated knockdown of MIP receptor expression blocked MIP-induced larval settlement behavior (Conzelmann et al., 2013); but see Discussion), and the hydrozoan cnidarian Hydractinia echinate, where RNAi-mediated knockdown of GLWamide precursor expression can decrease rates of metamorphosis, and synthetic GLWamide could rescue metamorphosis-less phenotype that resulted from pharmacological inhibition of neuropeptide amidation (Plickert et al., 2003). However, reduced rates of metamorphosis in GLWamide-deficient hydrozoan larvae were not consistently observed across independent experiments despite a near-complete loss of endogenous GLWamide precursor transcripts. It was therefore proposed that quantitatively small amounts of GLWamide might be sufficient for larvae to remain competent to metamorphosis induction. This hypothesis has yet to be directly confirmed by gene knockout approaches.

Here we used CRISPR-Cas9-mediated mutagenesis to generate knockout mutant lines for a Wamide precursor gene in the sea anemone cnidarian Nematostella vectensis, and examined the development of knockout mutants relative to the wildtype. In contrast to the hypothesis that Wamide is necessary for life cycle transition in H. echinate, we report that Wamide knockout mutant larvae can transform into morphologically normal polyps in N. vectensis. However, knockout mutant larvae undergo metamorphosis at slower rates than the wildtype control larvae, and this developmental phenotype could be rescued by treatment of the mutant larvae with synthetic GLWamide peptides. These results demonstrate that Wamide has a dispensable, modulatory role as an accelerator of metamorphosis in N. vectensis.

The genome of the sea anemone Nematostella vectensis (Putnam et al., 2007) encodes one Wamide (GLWamide, MH939200) precursor homolog (Nv126270) (Anctil, 2009). The GLWamide precursor gene was predicted to contain a single exon based on in silico gene prediction (http://genome.jgi.doe.gov/cgi-bin/dispGeneModel?db=Nemve1&id=126270); however, comparison of the N. vectensis genome and cDNA sequences instead shows that the GLWamide gene consists of two exons (Figure 1—figure supplement 1A). The analysis of putative endoproteolytic cleavage sites (i.e. acidic and basic amino acid residues; (Darmer et al., 1991; Leviev and Grimmelikhuijzen, 1995) and C-terminal amidation sites (i.e. C-terminal Gly residue) in the predicted precursor protein sequence suggests that the GLWamide gene encodes different copy numbers of nine distinct GLWamide peptides that vary in N-terminal sequence (Figure 1—figure supplement 1B,C). We note that previously documented NvLWamide-like gene (Nv242283; (Havrilak et al., 2017) is predicted to generate and release QCPPGLWGC peptides, but not GLWamides, because of the lack of the canonical amidation signal, glycine, at the C-terminus (see Figure 5—figure supplement 1 for experimental validation).

We first characterized the spatiotemporal expression patterns of GLWamide during planula development in N. vectensis. We used in situ hybridization and immunostaining to detect GLWamide precursor transcripts and GLWamide peptides, respectively. Consistent with previously published gene/peptide expression data (Nakanishi et al., 2012; Watanabe et al., 2014), GLWamide precursor transcripts were first detected in ectodermal sensory cells in the outer epithelium and in the pharynx during early-mid planula development (arrowheads in Figure 1A,B; inset in A) when a bundle of long cilia known as the apical tuft develops at the aboral pole. At the mid-planula stage, GLWamide transcripts appear in a subset of endodermal epithelial cells in the body column (arrowheads in Figure 1D). Interestingly, GLWamide-positive sensory cells in the pharynx were often asymmetrically distributed along the directive axis, oriented perpendicular to the oral-aboral axis (inset in Figure 1D; [Watanabe et al., 2014]).

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A confocal image stack used to generate panels A and B (dapi_blue actub_purple glw transcript_green).

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A confocal image stack used to generate panels C and D (dapi_blue actub_purple glw transcript_green).

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Immunostaining revealed GLWamide peptide expression in a number of neuronal processes of the ectodermal and endodermal nervous systems at the mid-late planula stage (Figure 2A,B). Ectodermal sensory cells of the body column extend basal processes aborally or laterally, but not toward the oral direction (Figure 2C,D), while GLWamide-positive pharyngeal sensory cells extend neurites in any direction (Figure 2E,F). In the endoderm, GLWamide expression is detected in neurons that form longitudinal neurite bundles (Figure 2G,H). These results demonstrate that, prior to transformation into a polyp through formation of oral tentacles, GLWamides are expressed in neurons and their neurites that constitute ectodermal and endodermal neural networks of the planula larva.

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A confocal image stack used to generate panels A, B, E and F (dapi_cyan actub_green GLWa_red).

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A confocal image stack used to generate panels C, D, G and H (dapi_cyan actub_green GLWa_red).

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Next we determined whether GLWamide neuropeptidergic input from the planula nervous system is required for metamorphic transition into a polyp in N. vectensis. To achieve this, we took a CRISPR-Cas9-mediated mutagenesis approach to generate animals with null alleles at the GLWamide locus. Several single guide RNA (sgRNA) species targeting the 5’ and 3’ regions of the gene (Materials and methods; Figure 3) were synthesized and microinjected with the endonuclease Cas9 into zygotes to produce double-strand DNA breaks at each of the target sites in the genome. This approach can create null mutant alleles at the target gene locus in two ways: (1) by introducing frameshift-causing indel mutations in the 5’ region of the gene via an error-prone, non-homologous end-joining (NHEJ) DNA repair mechanism, so that the translated peptide sequence is altered, and (2) by deleting the protein-coding region of the gene upon cleavages at flanking sites (cf. [Nakayama et al., 2013]). However, injected animals--here referred to as 'F0' animals--were usually mosaic mutants (Figure 3—figure supplement 1; [Servetnick et al., 2017]); mutagenesis occurred mosaically in an F0 embryo so that individual cells would carry different mutations, or no mutation at all. Therefore, it could not be assumed that every cell in an F0 animal harbored a null mutation at the locus of interest.

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We thus aimed to produce knockout mutant lines. First, F0 mosaic mutants were crossed with wild-type animals to generate F1 animals. Subsequently, F1 animals harboring a null mutant allele at the GLWamide locus were identified by genotyping each individual using genomic DNA extracted from pieces of oral tentacles. Upon screening, we found three different null mutant alleles at the GLWamide locus (glw^-a, glw^-b, and glw^-c), all of which carried frameshift mutations predicted to inhibit normal translation of neuropeptide precursor sequences (Figure 3A–D). glw F1 heterozygous mutants included one female with the genotype glw^-a/+ (i.e. wildtype), one female with the genotype glw^-b/+, one female with the genotype glw^-c/+, and four males with the genotype glw^-c/+. We then crossed F1 heterozygous mutants with one another to generate null mutants in, on average, a quarter of the F2 progeny. For instance, the glw^-b/+ female and the glw^-c/+ male were crossed to generate glw heterozygous null mutants with the genotype glw^-b/glw^-c.

To monitor development of null mutants, we first genotyped individual F2 embryos at the gastrula stage (i.e. at 1 dpf) (Figure 3E). Taking advantage of the ability of the oral half of the gastrula embryo to develop into a polyp (cf. [Fritzenwanker et al., 2007; Lee et al., 2007]), a piece of an aboral tissue of each F2 embryo was surgically separated and used for genomic DNA extraction, while the remaining embryo was individually maintained and allowed to regulate and develop into polyps. The genomic DNA extracted from an aboral tissue of each embryo was subsequently used as a template for PCR with allele-specific primers to determine the genotype of the embryo. The F2 embryos were then sorted by genotype, and null mutant and wildtype groups were used for subsequent analyses of development.

To determine whether GLWamide null mutants can undergo metamorphosis from a planula into a polyp in N. vectensis, we analyzed the morphology of the null mutants, glw^-b/glw^-c, and the wildtype control animals at 3dpf and 5dpf by using confocal microscopy. At 3dpf, glw^-b/glw^-c null mutants appeared to be morphologically identical to normal swimming planula larvae (Figure 4A–C,G–I), with an aboral apical tuft (at in Figure 4B,H) and an endoderm in which longitudinal muscle fibers are developing (arrowheads in Figure 4C,I). By 5dpf, the majority of glw^-b/glw^-c null mutants had transformed into morphologically normal primary polyps (glw^-b/glw^-c, 80% (n = 35); wildtype control, 78.6% (n = 56); Figure 4D–F,J–L). They show morphological hallmarks of metamorphosis, including the development of parietal and retractor muscles in the endoderm (pm and rm in Figure 4D,J), the loss of the apical tuft (arrowheads in Figure 4E,K; [Nakanishi et al., 2012]), and the formation of oral tentacles with polyp-specific cell types such as the hair cells (Figure 4F,L; (Nakanishi et al., 2012; Watson et al., 2009). Other glw null mutants--namely, glw^-a/glw^-c (F2) and glw^-a/glw^-a (F3)—also metamorphosed (data not shown). These results demonstrate that GLWamide of the larval nervous system is dispensable for metamorphosis in N. vectensis, and that cnidarian metamorphosis can occur via a GLWamide-independent mechanism.

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Because it has been proposed that the original function of (neuro)peptides, before emergence of the nervous system, was to regulate regenerative responses upon injury and/or stress as growth factors (Moroz, 2009), we also examined the regenerative capacity of GLWamide knockout mutants. F2 glw^-b/glw^-c and wildtype control juvenile polyps were transversely cut into oral and aboral halves, which were given two weeks to regenerate the aboral and oral regions, respectively. Regeneration occurred normally in glw^-b/glw^-c mutants; the oral half regenerated the aboral region (glw^-b/glw^-c, 100% (n = 6); wildtype control, 83.3% (n = 6), and the aboral half regenerated the oral structures such as the pharynx and oral tentacles (glw^-b/glw^-c, 100% (n = 6); wildtype control, 100% (n = 6). We also note that although pharmacological evidence suggests a role in oocyte maturation and spawning in a hydrozoan cnidarian (Takeda et al., 2013), GLWamide knockout mutants can generate functional gametes in response to light and temperature cues provided to induce spawning. Thus, GLWamides are not essential for regeneration or reproduction in N. vectensis.

We next considered the possibility that GLWamide might act to accelerate metamorphosis. We tested this alternative hypothesis by examining the time course of polyp formation in GLWamide null mutants relative to that in wildtype control animals. For this experiment, we crossed F2 glw^-a/glw^-c adults with each other to generate F3 null mutants, and F2 glw⁺/glw⁺ adults with each other to generate F3 wildtype control animals (Figure 5—figure supplement 1); glw^-a/glw^-c and glw⁺/glw⁺ F2 adults were siblings. Possible genotypes of null mutants examined were glw^-a/glw^-a, glw^-a/glw^-c, or glw^-c/glw^-c. Eggs of null mutants and wildtype control animals were fertilized by dropping egg packages into sperm-containing water at the same time (i.e. within a one-minute time window). Fertilized eggs were subsequently de-jellied, and animals were raised in constant darkness at 16°C; development at this temperature occurs slower than that at room temperature (20–25°C). At 2 dpf, healthy-appearing animals (i.e. without tissue damage) were transferred into wells of 24 well plates, each containing approximately 20 individuals in 1 ml of 1/3 seawater (wildtype, n = 6 experiments; mutants, n = 9 experiments). Half of the water in each well was replaced daily. For each experiment, we quantified the proportions of animals that were at the tentacle-bud or primary polyp stage from 6 dpf to 10 dpf. We observed no detectable difference in larval morphology at 5 dpf between null mutants and wildtype control animals, and thus we assume that larval development occurs normally at the morphological level. We used the number of polyps counted at 17 dpf as the total number of animals competent to become polyps for each experiment; the remaining animals that have not undergone metamorphosis by 17 dpf showed signs of abnormal development, and thus were excluded from the analysis. On average 17.5 animals, ranging from 14 to 20, were examined per experiment. We found that the wildtype control animals began to metamorphose at 6dpf, and the majority (~86%) had begun transforming by 8 dpf, while the null mutants began to metamorphose at 7dpf, and less than half (~42%) had begun metamorphosis by 8 dpf (Figure 5); the differences at 7 and 8 dpf were statistically significant (α = 0.05). Thus GLWamide null mutants metamorphosed at a slower rate than the wildtype control.

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Numerical data represented in Figure 5.

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To confirm that GLWamide deficiency is indeed responsible for this developmental delay, we tested whether treatment of GLWamide null mutants with synthetic GLWamide peptides (CEAGAPGLWamide), which were used to generate the anti-GLWamide antibody, would be sufficient to rescue the phenotype in developmental timing. Incubation of the mutants (n = 9 experiments) with 10 μM GLWamide began at 2 dpf and continued through 10 dpf with half of the peptide-containing media being replaced daily. On average 17.2 animals, ranging from 12 to 23, were examined per experiment. We observed that, similar to the wildtype animals, the null mutants treated with GLWamide began metamorphosis at 6 dpf, and the majority (~84%) had started metamorphosis by 8 dpf, showing statistically higher proportions of metamorphosing animals at 7 and 8 dpf relative to untreated null mutants (α = 0.05; Figure 5). Taken together, we conclude that GLWamide regulates metamorphosis by accelerating the process in N. vectensis.

Here we created the first neuropeptide knockout animals from an early-evolving animal group Cnidaria, and examined the endogenous function of deeply conserved (GL)Wamide neuropeptides in the sea anemone cnidarian Nematostella vectensis. We report that in N. vectensis GLWamide neuropeptides are expressed in ectodermal and endodermal nervous systems of the planula larva, but are not essential for transformation of the planula into a polyp. Instead we find that GLWamides regulate the timing of polyp formation by accelerating the metamorphic process in N. vectensis. These results raise the possibility that the ancestral role of Wamide neuropeptides in animals may have been to regulate the timing of life cycle transition.

Indeed, several lines of evidence indicate that the Wamide neuropeptide is an accelerator, but not an essential regulator, of metamorphosis in animals. First, as described above, exogenous Wamides can trigger life cycle transition across annelids and cnidarians (Conzelmann et al., 2013; Erwin and Szmant, 2010; Iwao et al., 2002; Leitz et al., 1994; Schmich et al., 1998b), and our results show slower rates of life cycle transition in GLWamide mutant larvae in sea anemones. These data support Wamide’s role as an accelerator of metamorphosis across animals.

Second, Wamides do not appear necessary for life cycle transition in animals where functional perturbation data are available. For instance, although in the annelid polychaete Platynereis, MIP (Wamide) receptor expression is necessary for MIP-induced larval settlement behavior (Conzelmann et al., 2013), reducing the expression of MIP by morpholino antisense oligonucleotide inhibits feeding behavior in the juvenile, but not metamorphosis (Williams et al., 2015). This could mean that there is a compensatory mechanism, possibly through as-yet-unknown ligands for the ‘MIP’ receptor. Alternative possibilities that silencing of the MIP gene was incomplete and the residual MIPs were sufficient for trigerring the transition, or that MIP does not endogenously regulate larval settlement and metamorphosis, cannot be ruled out, however; application of knockout approaches as employed in this study will be necessary to test these hypotheses. It is also possible that metamorphosis is decoupled from larval settlement so that MIP-regulated settlement behavior is not essential for metamorphosis in Platynereis. In the hydrozoan cnidarian Hydractinia echinata it is noteworthy that in one of the three experimental conditions where RNAi was used to knockdown GLWamide expression, the rate of metamorphosis was significantly reduced at 24 hr after induction (by CsCl), but it increased to a normal level 24 hr later (Plickert et al., 2003). This observation is consistent with a role of GLWamide as an accelerator, but not an essential regulator, of metamorphosis; however, an alternative possiblity that GLWamide expression recovered at later stages (due to reagent degradation and/or dilution) cannot be ruled out without gene knockout data. In the present study, our gene knockout data clearly demonstrate that GLWamides are not required for life cycle transition in the sea anemone cnidarian N. vectensis. We note that our data leave open the possibility that other amidated peptides could be necessary for metamorphosis in N. vectensis.

Third, animals that undergo metamorphosis do not necessarily have Wamide neuropeptides. For example, deuterostome bilaterians lack Wamide homologs (Conzelmann et al., 2013) despite metamorphosis being common in some of the lineages (e.g. echinoderms, hemichordates, ascidians, and vertebrates). Similarly, members of Porifera (sponges)—an outgroup to Eumetazoa—typically undergo metamorphosis, and yet Wamides have not been found in a sponge genome (Srivastava et al., 2010). Thus Wamide-independent mechanisms of metamorphosis must exist in some animal lineages.

Taken together, comparative evidence suggests that the ancestral Wamide neuropeptidergic signaling was not an indispensable component of the developmental regulatory system, but may have functioned to fine-tune the timing of life cycle transition by accelerating the developmental process—possibly in response to specific environmental cues—in the last common ancestor of Eumetazoa. Understanding how ancestral Wamides may have controlled developmental timing—the nature of the sensory stimuli that trigger a release of Wamides, and identities of Wamide receptors and target developmental regulatory genes, in particular—requires further investigation into Wamide function across Eumetazoa.

In addition to developmental timing, Wamides appear to be involved in regulating organismal behavior in extant animals. For instance, treatment with MIPs can suppress spontaneous contractions of the hindgut in insects (Schoofs et al., 1991). In the annelid Platynereis, in contrast, MIPs increase gut peristalsis and are necessary for feeding behavior (Williams et al., 2015). In cnidarians, exogenous GLWamides can induce muscle contractions in hydrozoan and anthozoan polyps (Takahashi et al., 2003) and stimulate migration of planula larvae in Hydractinia (Katsukura et al., 2004); however, it remains to be confirmed whether GLWamides endogenously control these behavioral processes. Demystifying the endogenous behavioral function(s) of deeply conserved neuropeptides—Wamides as well as others (e.g. RFamides)—across Eumetazoa will be important for illuminating how the primitive nervous system of the last common ancestor of Eumetazoa may have coordinated organismal behavior—another fundamental problem of neural evolution that remains enigmatic.

All data generated or analyzed during this study are included in the manuscript and supporting files. A source data file has been provided for Figures 1, 2 and 5. The GLWamide gene sequence has been deposited to GenBank under accession number MH939200.

1. 1. Nagayasu Nakanishi
   2. Mark Q Martindale

   (2018) GenBank

   ID MH939200. Nematostella vectensis GLWamide precursor, mRNA, complete cds.

   https://www.ncbi.nlm.nih.gov/nuccore/1483577241

1. 1. Anctil M

   (2009) Chemical transmission in the sea Anemone nematostella vectensis: a genomic perspective

   Comparative Biochemistry and Physiology Part D: Genomics and Proteomics 4:268–289.

   https://doi.org/10.1016/j.cbd.2009.07.001

   • PubMed
   • Google Scholar
2. 1. Conzelmann M
   2. Jékely G

   (2012) Antibodies against conserved amidated neuropeptide epitopes enrich the comparative neurobiology toolbox

   EvoDevo 3:23.

   https://doi.org/10.1186/2041-9139-3-23

   • PubMed
   • Google Scholar
3. 1. Conzelmann M
   2. Williams EA
   3. Tunaru S
   4. Randel N
   5. Shahidi R
   6. Asadulina A
   7. Berger J
   8. Offermanns S
   9. Jékely G

   (2013) Conserved MIP receptor-ligand pair regulates Platynereis larval settlement

   PNAS 110:8224–8229.

   https://doi.org/10.1073/pnas.1220285110

   • PubMed
   • Google Scholar
4. 1. Darmer D
   2. Schmutzler C
   3. Diekhoff D
   4. Grimmelikhuijzen CJ

   (1991) Primary structure of the precursor for the sea anemone neuropeptide Antho-RFamide (less than Glu-Gly-Arg-Phe-NH2)

   PNAS 88:2555–2559.

   https://doi.org/10.1073/pnas.88.6.2555

   • PubMed
   • Google Scholar
5. 1. Eipper BA
   2. Stoffers DA
   3. Mains RE

   (1992) The biosynthesis of neuropeptides: peptide alpha-amidation

   Annual Review of Neuroscience 15:57–85.

   https://doi.org/10.1146/annurev.ne.15.030192.000421

   • PubMed
   • Google Scholar
6. 1. Erwin PM
   2. Szmant AM

   (2010) Settlement induction of Acropora palmata planulae by a GLW-amide neuropeptide

   Coral Reefs 29:929–939.

   https://doi.org/10.1007/s00338-010-0634-1

   • Google Scholar
7. 1. Fritzenwanker JH
   2. Technau U

   (2002) Induction of gametogenesis in the basal cnidarian Nematostella vectensis(Anthozoa)

   Development Genes and Evolution 212:99–103.

   https://doi.org/10.1007/s00427-002-0214-7

   • PubMed
   • Google Scholar
8. 1. Fritzenwanker JH
   2. Genikhovich G
   3. Kraus Y
   4. Technau U

   (2007) Early development and axis specification in the sea anemone Nematostella vectensis

   Developmental Biology 310:264–279.

   https://doi.org/10.1016/j.ydbio.2007.07.029

   • PubMed
   • Google Scholar
9. 1. Grimmelikhuijzen CJP

   (1985) Antisera to the sequence Arg-Phe-amide visualize neuronal centralization in hydroid polyps

   Cell and Tissue Research 241:171–182.

   https://doi.org/10.1007/BF00214639

   • Google Scholar
10. 1. Grimmelikhuijzen CJP
    2. Williamson M
    3. Hansen GN

    (2002) Neuropeptides in cnidarians

    Canadian Journal of Zoology 80:1690–1702.

    https://doi.org/10.1139/z02-137

    • Google Scholar
11. 1. Grimmelikhuijzen CJ
    2. Hauser F

    (2012) Mini-review: the evolution of neuropeptide signaling

    Regulatory Peptides 177 Suppl:S6–S9.

    https://doi.org/10.1016/j.regpep.2012.05.001

    • PubMed
    • Google Scholar
12. 1. Hand C
    2. Uhlinger KR

    (1992) The culture, sexual and asexual reproduction, and growth of the sea Anemone Nematostella vectensis

    The Biological Bulletin 182:169–176.

    https://doi.org/10.2307/1542110

    • PubMed
    • Google Scholar
13. 1. Hartenstein V

    (2006) The neuroendocrine system of invertebrates: a developmental and evolutionary perspective

    Journal of Endocrinology 190:555–570.

    https://doi.org/10.1677/joe.1.06964

    • PubMed
    • Google Scholar
14. 1. Havrilak JA
    2. Faltine-Gonzalez D
    3. Wen Y
    4. Fodera D
    5. Simpson AC
    6. Magie CR
    7. Layden MJ

    (2017) Characterization of NvLWamide-like neurons reveals stereotypy in Nematostella nerve net development

    Developmental Biology 431:336–346.

    https://doi.org/10.1016/j.ydbio.2017.08.028

    • PubMed
    • Google Scholar
15. 1. Hua YJ
    2. Tanaka Y
    3. Nakamura K
    4. Sakakibara M
    5. Nagata S
    6. Kataoka H

    (1999) Identification of a prothoracicostatic peptide in the larval brain of the silkworm, Bombyx mori

    Journal of Biological Chemistry 274:31169–31173.

    https://doi.org/10.1074/jbc.274.44.31169

    • PubMed
    • Google Scholar
16. 1. Hwang WY
    2. Fu Y
    3. Reyon D
    4. Maeder ML
    5. Tsai SQ
    6. Sander JD
    7. Peterson RT
    8. Yeh JR
    9. Joung JK

    (2013) Efficient genome editing in zebrafish using a CRISPR-Cas system

    Nature Biotechnology 31:227–229.

    https://doi.org/10.1038/nbt.2501

    • PubMed
    • Google Scholar
17. 1. Ikmi A
    2. McKinney SA
    3. Delventhal KM
    4. Gibson MC

    (2014) TALEN and CRISPR/Cas9-mediated genome editing in the early-branching metazoan Nematostella vectensis

    Nature Communications 5:5486.

    https://doi.org/10.1038/ncomms6486

    • PubMed
    • Google Scholar
18. 1. Iwao K
    2. Fujisawa T
    3. Hatta M

    (2002) A cnidarian neuropeptide of the GLWamide family induces metamorphosis of reef-building corals in the genus Acropora

    Coral Reefs : Journal of the International Society for Reef Studies 21:127–129.

    https://doi.org/10.1007/s00338-002-0219-8

    • Google Scholar
19. 1. Jékely G

    (2013) Global view of the evolution and diversity of metazoan neuropeptide signaling

    PNAS 110:8702–8707.

    https://doi.org/10.1073/pnas.1221833110

    • PubMed
    • Google Scholar
20. 1. Katsukura Y
    2. Ando H
    3. David CN
    4. Grimmelikhuijzen CJ
    5. Sugiyama T

    (2004) Control of planula migration by LWamide and RFamide neuropeptides in Hydractinia echinata

    Journal of Experimental Biology 207:1803–1810.

    https://doi.org/10.1242/jeb.00974

    • PubMed
    • Google Scholar
21. 1. Koizumi O
    2. Sato N
    3. Goto C

    (2004) Chemical anatomy of hydra nervous system using antibodies against hydra neuropeptides: a review

    Hydrobiologia 530:41–47.

    https://doi.org/10.1007/s10750-004-2636-x

    • Google Scholar
22. 1. Lee PN
    2. Kumburegama S
    3. Marlow HQ
    4. Martindale MQ
    5. Wikramanayake AH

    (2007) Asymmetric developmental potential along the animal-vegetal axis in the anthozoan cnidarian, Nematostella vectensis, is mediated by Dishevelled

    Developmental Biology 310:169–186.

    https://doi.org/10.1016/j.ydbio.2007.05.040

    • PubMed
    • Google Scholar
23. 1. Leitz T
    2. Morand K
    3. Mann M

    (1994) Metamorphosin A: a novel peptide controlling development of the lower metazoan Hydractinia echinata (Coelenterata, Hydrozoa)

    Developmental Biology 163:440–446.

    https://doi.org/10.1006/dbio.1994.1160

    • PubMed
    • Google Scholar
24. 1. Leitz T
    2. Lay M

    (1995) Metamorphosin A is a neuropeptide

    Roux's Archives of Developmental Biology 204:276–279.

    https://doi.org/10.1007/BF00208495

    • Google Scholar
25. 1. Leviev I
    2. Grimmelikhuijzen CJ

    (1995) Molecular cloning of a preprohormone from sea anemones containing numerous copies of a metamorphosis-inducing neuropeptide: a likely role for dipeptidyl aminopeptidase in neuropeptide precursor processing

    PNAS 92:11647–11651.

    https://doi.org/10.1073/pnas.92.25.11647

    • PubMed
    • Google Scholar
26. 1. Lorenz MW
    2. Kellner R
    3. Hoffmann KH

    (1995) A family of neuropeptides that inhibit juvenile hormone biosynthesis in the cricket, Gryllus bimaculatus

    Journal of Biological Chemistry 270:21103–21108.

    https://doi.org/10.1074/jbc.270.36.21103

    • PubMed
    • Google Scholar
27. 1. Martindale MQ
    2. Pang K
    3. Finnerty JR

    (2004) Investigating the origins of triploblasty: 'mesodermal' gene expression in a diploblastic animal, the sea anemone Nematostella vectensis (phylum, Cnidaria; class, Anthozoa)

    Development 131:2463–2474.

    https://doi.org/10.1242/dev.01119

    • PubMed
    • Google Scholar
28. 1. Moroz LL

    (2009) On the independent origins of complex brains and neurons

    Brain, Behavior and Evolution 74:177–190.

    https://doi.org/10.1159/000258665

    • PubMed
    • Google Scholar
29. 1. Nakanishi N
    2. Hartenstein V
    3. Jacobs DK

    (2009) Development of the rhopalial nervous system in Aurelia sp.1 (Cnidaria, Scyphozoa)

    Development Genes and Evolution 219:301–317.

    https://doi.org/10.1007/s00427-009-0291-y

    • PubMed
    • Google Scholar
30. 1. Nakanishi N
    2. Renfer E
    3. Technau U
    4. Rentzsch F

    (2012) Nervous systems of the sea anemone Nematostella vectensis are generated by ectoderm and endoderm and shaped by distinct mechanisms

    Development 139:347–357.

    https://doi.org/10.1242/dev.071902

    • PubMed
    • Google Scholar
31. 1. Nakayama T
    2. Fish MB
    3. Fisher M
    4. Oomen-Hajagos J
    5. Thomsen GH
    6. Grainger RM

    (2013) Simple and efficient CRISPR/Cas9-mediated targeted mutagenesis in Xenopus tropicalis

    Genesis 51:835–843.

    https://doi.org/10.1002/dvg.22720

    • PubMed
    • Google Scholar
32. 1. Plickert G
    2. Schetter E
    3. Verhey-Van-Wijk N
    4. Schlossherr J
    5. Steinbüchel M
    6. Gajewski M

    (2003)

    The role of alpha-amidated neuropeptides in hydroid development--LWamides and metamorphosis in Hydractinia echinata

    The International Journal of Developmental Biology 47:439–450.

    • PubMed
    • Google Scholar
33. 1. Putnam NH
    2. Srivastava M
    3. Hellsten U
    4. Dirks B
    5. Chapman J
    6. Salamov A
    7. Terry A
    8. Shapiro H
    9. Lindquist E
    10. Kapitonov VV
    11. Jurka J
    12. Genikhovich G
    13. Grigoriev IV
    14. Lucas SM
    15. Steele RE
    16. Finnerty JR
    17. Technau U
    18. Martindale MQ
    19. Rokhsar DS

    (2007) Sea anemone genome reveals ancestral eumetazoan gene repertoire and genomic organization

    Science 317:86–94.

    https://doi.org/10.1126/science.1139158

    • PubMed
    • Google Scholar
34. 1. Schmich J
    2. Rudolf R
    3. Trepel S
    4. Leitz T

    (1998a) Immunohistochemical studies of GLWamides in Cnidaria

    Cell and Tissue Research 294:169–177.

    https://doi.org/10.1007/s004410051167

    • PubMed
    • Google Scholar
35. 1. Schmich J
    2. Trepel S
    3. Leitz T

    (1998b) The role of GLWamides in metamorphosis of Hydractinia echinata

    Development Genes and Evolution 208:267–273.

    https://doi.org/10.1007/s004270050181

    • PubMed
    • Google Scholar
36. 1. Schoofs L
    2. Holman GM
    3. Hayes TK
    4. Nachman RJ
    5. De Loof A

    (1991) Isolation, identification and synthesis of locustamyoinhibiting peptide (LOM-MIP), a novel biologically active neuropeptide from Locusta migratoria

    Regulatory Peptides 36:111–119.

    https://doi.org/10.1016/0167-0115(91)90199-Q

    • Google Scholar
37. 1. Schoofs L
    2. Beets I

    (2013) Neuropeptides control life-phase transitions

    PNAS 110:7973–7974.

    https://doi.org/10.1073/pnas.1305724110

    • PubMed
    • Google Scholar
38. 1. Servetnick MD
    2. Steinworth B
    3. Babonis LS
    4. Simmons D
    5. Salinas-Saavedra M
    6. Martindale MQ

    (2017) Cas9-mediated excision of Nematostella brachyury disrupts endoderm development, pharynx formation and oral-aboral patterning

    Development 144:2951–2960.

    https://doi.org/10.1242/dev.145839

    • PubMed
    • Google Scholar
39. 1. Srivastava M
    2. Simakov O
    3. Chapman J
    4. Fahey B
    5. Gauthier ME
    6. Mitros T
    7. Richards GS
    8. Conaco C
    9. Dacre M
    10. Hellsten U
    11. Larroux C
    12. Putnam NH
    13. Stanke M
    14. Adamska M
    15. Darling A
    16. Degnan SM
    17. Oakley TH
    18. Plachetzki DC
    19. Zhai Y
    20. Adamski M
    21. Calcino A
    22. Cummins SF
    23. Goodstein DM
    24. Harris C
    25. Jackson DJ
    26. Leys SP
    27. Shu S
    28. Woodcroft BJ
    29. Vervoort M
    30. Kosik KS
    31. Manning G
    32. Degnan BM
    33. Rokhsar DS

    (2010) The Amphimedon queenslandica genome and the evolution of animal complexity

    Nature 466:720–726.

    https://doi.org/10.1038/nature09201

    • PubMed
    • Google Scholar
40. Book

    1. Strand FL

    (1999)

    Neuropeptides: Regulators of Physiological Processes

    Cambridge: MIT Press.

    • Google Scholar
41. 1. Takahashi T
    2. Kobayakawa Y
    3. Muneoka Y
    4. Fujisawa Y
    5. Mohri S
    6. Hatta M
    7. Shimizu H
    8. Fujisawa T
    9. Sugiyama T
    10. Takahara M
    11. Yanagi K
    12. Koizumi O

    (2003) Identification of a new member of the GLWamide peptide family: physiological activity and cellular localization in cnidarian polyps

    Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 135:309–324.

    https://doi.org/10.1016/S1096-4959(03)00088-5

    • PubMed
    • Google Scholar
42. 1. Takeda N
    2. Nakajima Y
    3. Koizumi O
    4. Fujisawa T
    5. Takahashi T
    6. Matsumoto M
    7. Deguchi R

    (2013) Neuropeptides trigger oocyte maturation and subsequent spawning in the hydrozoan jellyfish Cytaeis uchidae

    Molecular Reproduction and Development 80:223–232.

    https://doi.org/10.1002/mrd.22154

    • PubMed
    • Google Scholar
43. Book

    1. Thomas M
    2. Edwards N

    (1991)

    Cnidaria: Hydrozoa

    In: FW H, editors. Microscopic Anatomy of Invertebrates. New York: Wiley-Liss. pp. 91–183.

    • Google Scholar
44. 1. Walker RJ
    2. Papaioannou S
    3. Holden-Dye L

    (2009) A review of FMRFamide- and RFamide-like peptides in metazoa

    Invertebrate Neuroscience 9:111–153.

    https://doi.org/10.1007/s10158-010-0097-7

    • PubMed
    • Google Scholar
45. 1. Watanabe H
    2. Kuhn A
    3. Fushiki M
    4. Agata K
    5. Özbek S
    6. Fujisawa T
    7. Holstein TW

    (2014) Sequential actions of β-catenin and Bmp pattern the oral nerve net in Nematostella vectensis

    Nature Communications 5:5536.

    https://doi.org/10.1038/ncomms6536

    • PubMed
    • Google Scholar
46. 1. Watson GM
    2. Mire P
    3. Kinler KM

    (2009) Mechanosensitivity in the model sea anemone Nematostella vectensis

    Marine Biology 156:2129–2137.

    https://doi.org/10.1007/s00227-009-1243-9

    • Google Scholar
47. 1. Williams EA
    2. Conzelmann M
    3. Jékely G

    (2015) Myoinhibitory peptide regulates feeding in the marine annelid Platynereis

    Frontiers in Zoology 12:1.

    https://doi.org/10.1186/s12983-014-0093-6

    • PubMed
    • Google Scholar

Author details

1. Nagayasu Nakanishi

   Department of Biological Sciences, University of Arkansas, Fayetteville, United States

   Contribution

   Conceptualization, Resources, Data curation, Formal analysis, Funding acquisition, Validation, Investigation, Methodology, Writing—original draft, Project administration, Writing—review and editing

   For correspondence

   nnakanis@uark.edu

   Competing interests

   No competing interests declared

   "This ORCID iD identifies the author of this article:" 0000-0001-7516-5078

2. Mark Q Martindale

   Whitney Laboratory for Marine Bioscience, University of Florida, St. Augustine, United States

   Contribution

   Conceptualization, Resources, Supervision, Funding acquisition, Methodology, Writing—review and editing

   Competing interests

   No competing interests declared

   "This ORCID iD identifies the author of this article:" 0000-0002-5805-5640

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