Molecular Epidemiology and Characterization of Genotypes of Acinetobacter baumannii Isolates from Regions of South China

Jun Ying; Junwan Lu; Li Zong; Ailing Li; Ruowang Pan; Cong Cheng; Kunpeng Li; Liqiang Chen; Jianchao Ying; Huifen Tou; Chuanxin Zhu; Teng Xu; Huiguang Yi; Jinsong Li; Liyan Ni; Zuyuan Xu; Qiyu Bao; Peizhen Li

doi:10.7883/yoken.JJID.2014.544

Original Article

Molecular Epidemiology and Characterization of Genotypes of Acinetobacter baumannii Isolates from Regions of South China

Jun Ying, Junwan Lu, Li Zong, Ailing Li, Ruowang Pan, Cong Cheng, Kunpeng Li, Liqiang Chen, Jianchao Ying, Huifen Tou, Chuanxin Zhu, Teng Xu, Huiguang Yi, Jinsong Li, Liyan Ni, Zuyuan Xu, Qiyu Bao, Peizhen Li

Author information

Jun Ying
School of Laboratory Medicine and Life Science/Institute of Biomedical Informatics, Wenzhou Medical University
Junwan Lu
School of Medicine, Lishui College
Li Zong
School of Laboratory Medicine and Life Science/Institute of Biomedical Informatics, Wenzhou Medical University
Ailing Li
School of Laboratory Medicine and Life Science/Institute of Biomedical Informatics, Wenzhou Medical University
Ruowang Pan
118 Hospital of PLA
Cong Cheng
School of Medicine, Lishui College
Kunpeng Li
School of Laboratory Medicine and Life Science/Institute of Biomedical Informatics, Wenzhou Medical University
Key Laboratory of Industrial Biotechnology of Ministry of Education and School of Biotechnology, Jiangnan University
Liqiang Chen
School of Laboratory Medicine and Life Science/Institute of Biomedical Informatics, Wenzhou Medical University
Institute of Neurobiology, Institutes of Brain Science and State Key Laboratory of Medical Neurobiology, Fudan University
Jianchao Ying
School of Laboratory Medicine and Life Science/Institute of Biomedical Informatics, Wenzhou Medical University
Huifen Tou
School of Laboratory Medicine and Life Science/Institute of Biomedical Informatics, Wenzhou Medical University
Wenzhou Center for Disease Control and Prevention
Chuanxin Zhu
Wenzhou Center for Disease Control and Prevention
Teng Xu
School of Laboratory Medicine and Life Science/Institute of Biomedical Informatics, Wenzhou Medical University
Huiguang Yi
School of Laboratory Medicine and Life Science/Institute of Biomedical Informatics, Wenzhou Medical University
Jinsong Li
School of Laboratory Medicine and Life Science/Institute of Biomedical Informatics, Wenzhou Medical University
Liyan Ni
The Second Affiliated Hospital, Wenzhou Medical University
Zuyuan Xu
School of Laboratory Medicine and Life Science/Institute of Biomedical Informatics, Wenzhou Medical University
Qiyu Bao
School of Laboratory Medicine and Life Science/Institute of Biomedical Informatics, Wenzhou Medical University
Peizhen Li
School of Laboratory Medicine and Life Science/Institute of Biomedical Informatics, Wenzhou Medical University

Keywords: Acinetobacter baumannii, drug resistance profiles, molecular subtyping, multilocus sequence typing, pulsed-field gel electrophoresis

JOURNAL FREE ACCESS

2016 Volume 69 Issue 3 Pages 180-185

DOI https://doi.org/10.7883/yoken.JJID.2014.544

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Abstract

The aim of this study was to analyze the molecular epidemiologic characteristics of Acinetobacter baumannii. A total of 398 isolates were collected in 7 regions of South China from January to June of 2012. Drug sensitivity was tested toward 15 commonly used antibiotics; thus, 146 multi-drug-resistant strains (resistant to more than 7 drugs) were identified, representing 36.7% of all isolates. Pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) were used for molecular subtyping. According to the PFGE results (with a cutoff of 70% similarity for the DNA electrophoretic bands), 146 strains were subdivided into 15 clusters, with cluster A being the largest (33.6%, distributed in all districts except Jiaxing). Cluster B was also widespread and included 14.4% of all strains. In addition, MLST results revealed 11 sequence types (ST), with ST208 being the most prevalent, followed by ST191 and ST729. Furthermore, 4 novel alleles and 6 novel STs were identified. Our results showed that multi-drug-resistant A. baumannii in South China shares the origin with other widespread strains in other countries. The nosocomial infections caused by A. baumannii have been severe in South China. Continuous monitoring and judicious antibiotic use are required.

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