Fecal Microbiome Composition in Healthy Adults in Ghana

Prince Kofi Parbie; Taketoshi Mizutani; Aya Ishizaka; Ai Kawana-Tachikawa; Lucky Ronald Runtuwene; Sayuri Seki; Christopher Zaab-Yen Abana; Dennis Kushitor; Evelyn Yayra Bonney; Sampson Badu Ofori; Satoshi Uematsu; Seiya Imoto; Yasumasa Kimura; Hiroshi Kiyono; Koichi Ishikawa; William Kwabena Ampofo; Tetsuro Matano

doi:10.7883/yoken.JJID.2020.469

Original Articles

Fecal Microbiome Composition in Healthy Adults in Ghana

Prince Kofi Parbie, Taketoshi Mizutani, Aya Ishizaka, Ai Kawana-Tachikawa, Lucky Ronald Runtuwene, Sayuri Seki, Christopher Zaab-Yen Abana, Dennis Kushitor, Evelyn Yayra Bonney, Sampson Badu Ofori, Satoshi Uematsu, Seiya Imoto, Yasumasa Kimura, Hiroshi Kiyono, Koichi Ishikawa, William Kwabena Ampofo, Tetsuro Matano

Author information

Prince Kofi Parbie
Joint Research Center for Human Retrovirus Infection, Kumamoto University, Japan
AIDS Research Center, National Institute of Infectious Diseases, Japan
Noguchi Memorial Institute for Medical Research, University of Ghana, Ghana
Taketoshi Mizutani
The Institute of Medical Science, The University of Tokyo, Japan
Aya Ishizaka
The Institute of Medical Science, The University of Tokyo, Japan
Ai Kawana-Tachikawa
Joint Research Center for Human Retrovirus Infection, Kumamoto University, Japan
AIDS Research Center, National Institute of Infectious Diseases, Japan
The Institute of Medical Science, The University of Tokyo, Japan
Lucky Ronald Runtuwene
AIDS Research Center, National Institute of Infectious Diseases, Japan
Sayuri Seki
AIDS Research Center, National Institute of Infectious Diseases, Japan
Christopher Zaab-Yen Abana
Noguchi Memorial Institute for Medical Research, University of Ghana, Ghana
Dennis Kushitor
Noguchi Memorial Institute for Medical Research, University of Ghana, Ghana
Evelyn Yayra Bonney
Noguchi Memorial Institute for Medical Research, University of Ghana, Ghana
Sampson Badu Ofori
Regional Hospital Koforidua, Ghana Health Service, Ghana
Satoshi Uematsu
The Institute of Medical Science, The University of Tokyo, Japan
Department of Immunology and Genomics, Osaka City University Graduate School of Medicine, Japan
Collaborative Research Institute for Innovative Microbiology, The University of Tokyo, Japan
Seiya Imoto
The Institute of Medical Science, The University of Tokyo, Japan
Collaborative Research Institute for Innovative Microbiology, The University of Tokyo, Japan
Yasumasa Kimura
The Institute of Medical Science, The University of Tokyo, Japan
Hiroshi Kiyono
The Institute of Medical Science, The University of Tokyo, Japan
Institute for Global Prominent Research, Graduate School of Medicine, Chiba University, Japan
CU-UCSD Center for Mucosal Immunology, Allergy and Vaccines (cMAV), Department of Medicine, University of California San Diego, USA
Koichi Ishikawa
AIDS Research Center, National Institute of Infectious Diseases, Japan
William Kwabena Ampofo
Noguchi Memorial Institute for Medical Research, University of Ghana, Ghana
Tetsuro Matano
Joint Research Center for Human Retrovirus Infection, Kumamoto University, Japan
AIDS Research Center, National Institute of Infectious Diseases, Japan
The Institute of Medical Science, The University of Tokyo, Japan

Keywords: gut microbiome, diversity, West Africa, Ghana

JOURNAL FREE ACCESS

2021 Volume 74 Issue 1 Pages 42-47

DOI https://doi.org/10.7883/yoken.JJID.2020.469

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Abstract

Recent studies have indicated an association between gut microbiome composition and various disorders, including infectious diseases. The composition of the microbiome differs among ethnicities and countries, possibly resulting in diversified interactions between host immunity and the gut microbiome. Characterization of baseline microbiome composition in healthy people is an essential step for better understanding of the biological interactions associated with individual populations. However, data on the gut/fecal microbiome have not been accumulated for individuals in West Africa. In the present study, we examined the fecal microbiome composition in healthy adults in Ghana. Toward this, 16S rRNA gene libraries were prepared using bacterial fractions derived from 55 Ghanaian adults, which were then subjected to next-generation sequencing. The fecal microbiome of the Ghanaian adults was dominated by Firmicutes (Faecalibacterium, Subdoligranulum, and Ruminococcaceae UCG-014), Proteobacteria (Escherichia-Shigella and Klebsiella), and Bacteroidetes (Prevotella 9 and Bacteroides), consistent with previous observations in African cohorts. Further, our analysis revealed differences in microbiome composition and a lower diversity of the fecal microbiome in the Ghanaian cohort compared with those reported in non-African countries. This is the first study to describe substantial fecal microbiome data obtained using high-throughput metagenomic tools on samples derived from a cohort in Ghana. The data may provide a valuable basis for determining the association between the fecal microbiome and progression of various diseases in West African populations.

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