Analysis of Physiological Indicators Associated with Drought Tolerance in Wheat under Drought and Re-Watering Conditions
Abstract
:1. Introduction
2. Materials and Methods
2.1. Experimental Site and Growth Conditions
2.2. Experimental Design and Plant Materials
2.2.1. Photosynthetic Gas Exchange Parameters
2.2.2. Chlorophyll Fluorescence Parameters
2.2.3. Biochemical Parameters
2.2.4. Grain Yield
2.3. Statistical Analysis
3. Results
3.1. Effects of Drought Stress and Re-Watering on Yield and Phenotype
3.2. Changes in the Photosynthetic Characteristics and Chlorophyll Fluorescence Parameters under Drought Stress and Re-Watering Conditions
3.3. Changes in Antioxidant Enzyme Activity, Osmoregulatory Substances, and Sugar Content under Drought Stress and Re-Watering Conditions
3.4. Analysis of Variance
3.5. Comprehensive Evaluation of Wheat Drought Tolerance
3.5.1. Drought Tolerance Coefficient of Individual Indicators and Correlation Analysis
3.5.2. Principal Component Analysis (PCA)
3.6. Screening of Drought Tolerance Indicators
3.6.1. Grey Relational Analysis
3.6.2. Construction of a Regression Model
4. Discussion
4.1. Physiological Responses of Wheat to Different Durations of Drought
4.2. Compensatory Effect of Re-Watering on Wheat Physiological Characteristics
4.3. Screening and Identification of Physiological Indices for Drought Tolerance in Wheat
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Treatment | LH22 Yield (g pot−1) | ZM366 Yield (g pot−1) |
---|---|---|
WW | 47.67 ± 0.70 a | 51.80 ± 0.53 a |
D | 23.85 ±1.96 d | 21.08 ± 1.59 d |
R6 | 38.87 ± 1.65 b | 36.58 ± 1.65 b |
R12 | 32.96 ± 0.56 c | 29.73 ± 0.45 c |
R18 | 25.79 ± 1.00 d | 23.47 ± 0.60 d |
Pn | Gs | SOD | Pro | MDA | SS | HXKs | GLC | Fv/Fm | qP | NPQ | qN | |
---|---|---|---|---|---|---|---|---|---|---|---|---|
Soil Moisture (M) | * | *** | *** | *** | *** | *** | *** | *** | *** | *** | *** | *** |
Cultivar (C) | ns | *** | ns | * | *** | ns | ** | * | *** | ** | * | *** |
Stress Duration (SD) | *** | *** | *** | *** | *** | *** | *** | *** | *** | *** | *** | *** |
M× C | * | ** | ns | ns | *** | ns | ns | * | ** | ns | ns | ns |
M × SD | *** | *** | ns | * | ns | ** | ** | ** | * | ns | * | ns |
C × SD | ns | ** | ns | ns | * | ns | ns | ns | *** | ns | ns | ns |
M × C × SD | ns | ns | ns | ns | ns | * | ns | ns | ** | ns | ns | ns |
Index | Factor Loading | |
---|---|---|
F1 | F2 | |
Pn | −0.808 | 0.418 |
Gs | −0.892 | 0.088 |
SOD | 0.809 | −0.448 |
Pro | 0.899 | 0.092 |
MDA | 0.836 | 0.443 |
SS | 0.937 | 0.015 |
HXKs | 0.717 | 0.019 |
GLC | 0.845 | 0.369 |
Fv/Fm | −0.959 | −0.060 |
qP | −0.929 | −0.105 |
NPQ | 0.916 | −0.202 |
qN | 0.948 | 0.018 |
Characteristic root | 9.235 | 0.780 |
Contribution rate (%) | 76.957 | 6.504 |
Cumulative contribution rate (%) | 76.957 | 83.461 |
Factor weight | 0.922 | 0.078 |
Index | Correlation Degree with D Value | Rank | Weight | Correlation Degree with WDC Value | Rank | |
---|---|---|---|---|---|---|
X1 | Pn | 0.643 | 9 | 0.077 | 0.689 | 10 |
X2 | Gs | 0.562 | 12 | 0.067 | 0.542 | 12 |
X3 | SOD | 0.707 | 5 | 0.085 | 0.877 | 3 |
X4 | Pro | 0.750 | 3 | 0.090 | 0.800 | 6 |
X5 | MDA | 0.767 | 2 | 0.092 | 0.743 | 7 |
X6 | SS | 0.828 | 1 | 0.099 | 0.679 | 11 |
X7 | HXKs | 0.692 | 8 | 0.083 | 0.851 | 5 |
X8 | GLC | 0.729 | 4 | 0.087 | 0.864 | 4 |
X9 | Fv/Fm | 0.642 | 10 | 0.077 | 0.726 | 8 |
X10 | qP | 0.630 | 11 | 0.075 | 0.694 | 9 |
X11 | NPQ | 0.704 | 6 | 0.084 | 0.879 | 2 |
X12 | qN | 0.704 | 7 | 0.084 | 0.917 | 1 |
Dependent Variable | Multiple Stepwise Regression Equation | R2 | p |
---|---|---|---|
D value | y = −0.492−0.148X2 + 0.136X4 + 0.108X5 + 0.105X6 + 0.076X7 + 0.091X8 + 0.135X11 | 0.999 | 0.0001 ** |
CDC value | y = 0.274 + 0.071X1 + 0.098X2 + 0.095X4 + 0.074X5 + 0.088X6 + 0.106X7 + 0.062X8 + 0.139X11 | 0.998 | 0.0001 ** |
WDC value | y = 0.333 + 0.098X2 + 0.093X4 + 0.087X5 + 0.108X6 + 0.107X7 + 0.069X8 + 0.107X11 | 0.998 | 0.0001 ** |
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Wang, J.; Zhang, X.; Han, Z.; Feng, H.; Wang, Y.; Kang, J.; Han, X.; Wang, L.; Wang, C.; Li, H.; et al. Analysis of Physiological Indicators Associated with Drought Tolerance in Wheat under Drought and Re-Watering Conditions. Antioxidants 2022, 11, 2266. https://0-doi-org.brum.beds.ac.uk/10.3390/antiox11112266
Wang J, Zhang X, Han Z, Feng H, Wang Y, Kang J, Han X, Wang L, Wang C, Li H, et al. Analysis of Physiological Indicators Associated with Drought Tolerance in Wheat under Drought and Re-Watering Conditions. Antioxidants. 2022; 11(11):2266. https://0-doi-org.brum.beds.ac.uk/10.3390/antiox11112266
Chicago/Turabian StyleWang, Jiarui, Xiaoyan Zhang, Zhidong Han, Haoxiang Feng, Yangyang Wang, Juan Kang, Xiaojie Han, Lifang Wang, Chenyang Wang, Hua Li, and et al. 2022. "Analysis of Physiological Indicators Associated with Drought Tolerance in Wheat under Drought and Re-Watering Conditions" Antioxidants 11, no. 11: 2266. https://0-doi-org.brum.beds.ac.uk/10.3390/antiox11112266