Effect of Heat Stress on Bovine Mammary Cellular Metabolites and Gene Transcription Related to Amino Acid Metabolism, Amino Acid Transportation and Mammalian Target of Rapamycin (mTOR) Signaling
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Cell Culture and Treatments
2.2. RNA Extraction and Real-Time PCR Analysis
2.3. LC-MS Analysis
2.4. Data Analysis
3. Results
3.1. Heat Shock Response of MAC-T Cells
3.2. Intracellular Metabolism of MAC-T Cells
3.3. Milk Protein Synthesis Regulation of MAC-T Cells
3.4. Casein Synthesis of MAC-T Cells
4. Discussion
4.1. Heat Stress Model Construction
4.2. Metabolomics
4.2.1. Glutathione Metabolism
4.2.2. Phenylalanine, Tyrosine and Tryptophan Metabolism
4.2.3. Valine, Leucine and Isoleucine Metabolism
4.2.4. Arginine and Proline Metabolism
4.2.5. Alanine, Aspartate and Glutamate Metabolism
4.2.6. Cysteine and Methionine Metabolism
4.3. Amino Acid Transporters
4.4. mTOR Signaling Pathway
4.5. Milk Protein Synthesis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
References
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Fu, L.; Zhang, L.; Liu, L.; Yang, H.; Zhou, P.; Song, F.; Dong, G.; Chen, J.; Wang, G.; Dong, X. Effect of Heat Stress on Bovine Mammary Cellular Metabolites and Gene Transcription Related to Amino Acid Metabolism, Amino Acid Transportation and Mammalian Target of Rapamycin (mTOR) Signaling. Animals 2021, 11, 3153. https://0-doi-org.brum.beds.ac.uk/10.3390/ani11113153
Fu L, Zhang L, Liu L, Yang H, Zhou P, Song F, Dong G, Chen J, Wang G, Dong X. Effect of Heat Stress on Bovine Mammary Cellular Metabolites and Gene Transcription Related to Amino Acid Metabolism, Amino Acid Transportation and Mammalian Target of Rapamycin (mTOR) Signaling. Animals. 2021; 11(11):3153. https://0-doi-org.brum.beds.ac.uk/10.3390/ani11113153
Chicago/Turabian StyleFu, Lin, Li Zhang, Li Liu, Heng Yang, Peng Zhou, Fan Song, Guozhong Dong, Juncai Chen, Gaofu Wang, and Xianwen Dong. 2021. "Effect of Heat Stress on Bovine Mammary Cellular Metabolites and Gene Transcription Related to Amino Acid Metabolism, Amino Acid Transportation and Mammalian Target of Rapamycin (mTOR) Signaling" Animals 11, no. 11: 3153. https://0-doi-org.brum.beds.ac.uk/10.3390/ani11113153