Development of an Internet of Things Based Electrochemical Microfluidic System for Free Calcium Detection
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials and Apparatus
2.2. Multi-Sensing Electrode Interdigital Sensor and Electrochemical Impedance Spectroscopy (EIS) Measurement
2.3. Fabrication of Microfluidics Detection System
2.4. Preparation of the Calcium Samples
2.5. Experimental Measurements
2.6. Electrochemical Impedance Monitoring Using the Microcontroller-Based System
2.6.1. Block Diagram of the Point-of-Care System
2.6.2. Circuit Diagram of the Proposed System
2.6.3. Software Process Flow of the PoC Device
3. Results and Discussion
3.1. Detection of the Reactance Measurement of the LCR Meter
3.2. Measurement of the Reactance Measurement with PoC Device
3.3. IoT Data from the Point-of-Care Device
4. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
- Feng, S.; Dong, T.; Yang, Z. Detection of urinary tract infections on lab-on-chip device by measuring photons emitted from atp bioluminescence. In Proceedings of the 36th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC), Chicago, IL, USA, 26–30 August 2014; pp. 3114–3117. [Google Scholar]
- Feng, S.; Dong, T. Design and characterization of a lab-on-chip for continuous bioluminescent measurements of atp. In Proceedings of the 2014 IEEE International Symposium on Medical Measurements and Applications (MeMeA), Lisboa, Portugal, 11–12 June 2014; pp. 1–4. [Google Scholar]
- Feng, S.L.; Dong, T. Applied technology in diaper-based uti testing for elder people by using nitrite ion selective electrode. Appl. Mech. Mater. 2014, 662, 225–228. [Google Scholar] [CrossRef]
- Feng, S.; Roseng, L.E.; Dong, T. Quantitative detection of escherichia coli and measurement of urinary tract infection diagnosis possibility by use of a portable, handheld sensor. In Proceedings of the 2015 IEEE International Symposium on Medical Measurements and Applications (MeMeA), Turin, Italy, 7–9 May 2015; pp. 586–589. [Google Scholar]
- Feng, S. Investigations of Paper-based Lab-on-Chips for On-Diaper Point-of-Care Screening of Urinary Tract Infections. Master′s Thesis, Universitetet i Sørøst-Norge, Porsgrunn, Norway, 2015. [Google Scholar]
- Nag, A.; Zia, A.I.; Li, X.; Mukhopadhyay, S.C.; Kosel, J. Novel sensing approach for lpg leakage detection—Part ii: Effects of particle size, composition, and coating layer thickness. IEEE Sens. J. 2016, 16, 1088–1094. [Google Scholar] [CrossRef]
- Rahman, M.S.A.; Mukhopadhyay, S.C.; Yu, P.-L. Novel planar interdigital sensors. In Novel Sensors for Food Inspection: Modelling, Fabrication and Experimentation; Springer: Berlin/Heidelberg, Germany, 2014; Volume 10, pp. 11–35. [Google Scholar]
- Mohd Syaifudin, A.; Yunus, M.; Mukhopadhyay, S.; Jayasundera, K. A novel planar interdigital sensor for environmental monitoring. In Proceedings of the 2009 IEEE SENSORS, Christchurch, New Zealand, 25–28 October 2009; pp. 105–110. [Google Scholar]
- Syaifudin, A.M.; Jayasundera, K.; Mukhopadhyay, S. A low cost novel sensing system for detection of dangerous marine biotoxins in seafood. Sens. Actuat. B-Chem. 2009, 137, 67–75. [Google Scholar] [CrossRef]
- Afsarimanesh, N.; Mukhopadhyay, S.C.; Kruger, M. Molecularly imprinted polymer-based electrochemical biosensor for bone loss detection. IEEE Trans. Biomed. Eng. 2018, 65, 1264–1267. [Google Scholar] [CrossRef] [PubMed]
- Beebe, D.J.; Denton, D.D.; Radwin, R.G.; Webster, J.G. A silicon-based tactile sensor for finger-mounted applications. IEEE Trans. Biomed. Eng. 1998, 45, 151–159. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Nambiar, S.; Yeow, J.T. Conductive polymer-based sensors for biomedical applications. Biosens. Bioelectron. 2011, 26, 1825–1832. [Google Scholar] [CrossRef] [PubMed]
- Pramanik, C.; Saha, H.; Gangopadhyay, U. Design optimization of a high performance silicon mems piezoresistive pressure sensor for biomedical applications. J. Micromech. Microeng. 2006, 16, 2060. [Google Scholar] [CrossRef]
- Errachid, A.; Ivorra, A.; Aguilo, J.; Villa, R.; Zine, N.; Bausells, J. New technology for multi-sensor silicon needles for biomedical applications. Sens. Actuat. B-Chem. 2001, 78, 279–284. [Google Scholar] [CrossRef]
- Nag, A.; Afasrimanesh, N.; Feng, S.; Mukhopadhyay, S.C. Strain induced graphite/pdms sensors for biomedical applications. Sens. Actuat. A-Phys. 2018, 271, 257–269. [Google Scholar] [CrossRef]
- Vashist, S.K. Point-of-care diagnostics: Recent advances and trends. Biosensors 2017, 7, 62. [Google Scholar] [CrossRef] [PubMed]
- Khan, M.S.; Misra, S.K.; Wang, Z.; Daza, E.; Schwartz-Duval, A.S.; Kus, J.M.; Pan, D.; Pan, D. Based analytical biosensor chip designed from graphene-nanoplatelet-amphiphilic-diblock-co-polymer composite for cortisol detection in human saliva. Anal. Chem. 2017, 89, 2107–2115. [Google Scholar] [CrossRef] [PubMed]
- Uludag, Y.; Narter, F.; Sağlam, E.; Köktürk, G.; Gök, M.Y.; Akgün, M.; Barut, S.; Budak, S. An integrated lab-on-a-chip-based electrochemical biosensor for rapid and sensitive detection of cancer biomarkers. Anal. Bioanal. Chem. 2016, 408, 7775–7783. [Google Scholar] [CrossRef] [PubMed]
- Wu, Y.; Xue, P.; Kang, Y.; Hui, K.M. Based microfluidic electrochemical immunodevice integrated with nanobioprobes onto graphene film for ultrasensitive multiplexed detection of cancer biomarkers. Anal. Chem. 2013, 85, 8661–8668. [Google Scholar] [CrossRef] [PubMed]
- Wu, Y.; Xue, P.; Hui, K.M.; Kang, Y. A paper-based microfluidic electrochemical immunodevice integrated with amplification-by-polymerization for the ultrasensitive multiplexed detection of cancer biomarkers. Biosens. Bioelectron. 2014, 52, 180–187. [Google Scholar] [CrossRef] [PubMed]
- Korotcenkov, G.; Brinzari, V.; Cho, B.K. Conductometric gas sensors based on metal oxides modified with gold nanoparticles: A review. Microchim. Acta 2016, 183, 1033–1054. [Google Scholar] [CrossRef]
- Hung, D.Q.; Nekrassova, O.; Compton, R.G. Analytical methods for inorganic arsenic in water: A review. Talanta 2004, 64, 269–277. [Google Scholar] [CrossRef] [PubMed]
- Alahi, M.E.E.; Li, X.; Mukhopadhyay, S.; Burkitt, L. A temperature compensated smart nitrate-sensor for agricultural industry. IEEE Trans. Ind. Electron. 2017, 64, 7333–7341. [Google Scholar] [CrossRef]
- Khan, M.S.; Misra, S.K.; Schwartz-Duval, A.S.; Daza, E.; Ostadhossein, F.; Bowman, M.; Jain, A.; Taylor, G.; McDonagh, D.; Labriola, L.T. Real-time monitoring of post-surgical and post-traumatic eye injuries using multilayered electrical biosensor chip. ACS Appl. Mater. Interfaces 2017, 9, 8609–8622. [Google Scholar] [CrossRef] [PubMed]
- Nukala, B.T.; Nakano, T.; Rodriguez, A.; Tsay, J.; Lopez, J.; Nguyen, T.Q.; Zupancic, S.; Lie, D.Y. Real-time classification of patients with balance disorders vs. Normal subjects using a low-cost small wireless wearable gait sensor. Biosensors 2016, 6, 58. [Google Scholar] [CrossRef] [PubMed]
- Sharma, S.; Zapatero-Rodríguez, J.; Estrela, P.; O′Kennedy, R. Point-of-care diagnostics in low resource settings: Present status and future role of microfluidics. Biosensors 2015, 5, 577–601. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Sheikh, N.J.; Sheikh, O. Forecasting of biosensor technologies for emerging point of care and medical iot applications using bibliometrics and patent analysis. In Proceedings of the 2016 Portland International Conference on Management of Engineering and Technology (PICMET), Honolulu, HI, USA, 4–8 September 2016; pp. 3082–3093. [Google Scholar]
- Mora, H.; Gil, D.; Terol, R.M.; Azorín, J.; Szymanski, J. An iot-based computational framework for healthcare monitoring in mobile environments. Sensors 2017, 17, 2302. [Google Scholar] [CrossRef] [PubMed]
- Islam, S.M.R.; Kwak, D.; Kabir, M.H.; Hossain, M.; Kwak, K.S. The internet of things for health care: A comprehensive survey. IEEE Access 2015, 3, 678–708. [Google Scholar] [CrossRef]
- Smeets, W.T.G.; Seekles, L. Determination of the content of calcium ions in milk ultrafiltrate. Nature 1952, 169, 802. [Google Scholar] [CrossRef] [PubMed]
- Nirschl, M.; Ottl, J.; Vörös, J. Conformational changes of calmodulin on calcium and peptide binding monitored by film bulk acoustic resonators. Biosensors 2011, 1, 164–176. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Pinton, P.; Rimessi, A.; Romagnoli, A.; Prandini, A.; Rizzuto, R. Biosensors for the detection of calcium and pH. Methods Cell Biol. 2007, 80, 297–325. [Google Scholar] [PubMed]
- Mamishev, A.V.; Sundara-Rajan, K.; Fumin, Y.; Yanqing, D.; Zahn, M. Interdigital sensors and transducers. Proc. IEEE 2004, 92, 808–845. [Google Scholar] [CrossRef] [Green Version]
- Mamishev, A.V.; Du, Y.; Bau, J.H.; Lesieutre, B.C.; Zahn, M. Evaluation of diffusion-driven material property profiles using three-wavelength interdigital sensor. IEEE Trans. Dielectr. Electr. Insul. 2001, 8, 785–798. [Google Scholar] [CrossRef] [Green Version]
- Khan, M.S.; Misra, S.K.; Dighe, K.; Wang, Z.; Schwartz-Duval, A.S.; Sar, D.; Pan, D. Electrically-receptive and thermally-responsive paper-based sensor chip for rapid detection of bacterial cells. Biosens. Bioelectron. 2018, 110, 132–140. [Google Scholar] [CrossRef] [PubMed]
- Zia, A.I.; Rahman, M.S.A.; Mukhopadhyay, S.C.; Yu, P.-L.; Al-Bahadly, I.H.; Gooneratne, C.P.; Kosel, J.; Liao, T.-S. Technique for rapid detection of phthalates in water and beverages. J. Food Eng. 2013, 116, 515–523. [Google Scholar] [CrossRef]
- Thingspeak. Available online: https://thingspeak.com/ (accessed on 26 August 2017).
- Arduino. Available online: https://www.arduino.cc/en/Reference/Ciao (accessed on 26 August 2017).
- Arduino Uno Wifi. Available online: https://store.arduino.cc/usa/arduino-uno-wifi (accessed on 4 September 2017).
- Devices, A. Ad5933: Impedance Analyzer. Available online: http://www.analog.com/media/en/technical-documentation/data-sheets/AD5933.pdf (accessed on 4 September 2017).
- Devices, A. Adg849. Available online: http://www.analog.com/media/en/technical-documentation/data-sheets/ADG849.pdf (accessed on 8 September 2017).
- Device, A. Direct Digital Synthesis. Available online: http://www.analog.com/media/en/analog-dialogue/volume-38/number-3/articles/all-about-direct-digital-synthesis.pdf (accessed on 8 September 2017).
- Mankar, J.; Darode, C.; Trivedi, K.; Kanoje, M.; Shahare, P. Review of i2c protocol. Int. J. 2014, 2, 474–479. [Google Scholar]
- Arduino. Available online: https://www.arduino.cc/en/Guide/Environment (accessed on 10 July 2018).
- Manickam, A.; Johnson, C.A.; Kavusi, S.; Hassibi, A. Interface design for cmos-integrated electrochemical impedance spectroscopy (eis) biosensors. Sensors 2012, 12, 14467–14488. [Google Scholar] [CrossRef] [PubMed]
- Scott, D.W.; Alseiha, Y. Determining detection limits of aqueous anions using electrochemical impedance spectroscopy. J. Anal. Sci. Technol. 2017, 8, 17. [Google Scholar] [CrossRef]
- Khan, M.S.; Dosoky, N.S.; Mustafa, G.; Patel, D.; Berdiev, B.; Williams, J.D. Electrophysiology of epithelial sodium channel (enac) embedded in supported lipid bilayer using a single nanopore chip. Langmuir 2017, 33, 13680–13688. [Google Scholar] [CrossRef] [PubMed]
© 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Share and Cite
Yuan, Y.; Feng, S.; Alahi, M.E.E.; Nag, A.; Afsarimanesh, N.; Zhang, H.; He, S. Development of an Internet of Things Based Electrochemical Microfluidic System for Free Calcium Detection. Appl. Sci. 2018, 8, 1357. https://0-doi-org.brum.beds.ac.uk/10.3390/app8081357
Yuan Y, Feng S, Alahi MEE, Nag A, Afsarimanesh N, Zhang H, He S. Development of an Internet of Things Based Electrochemical Microfluidic System for Free Calcium Detection. Applied Sciences. 2018; 8(8):1357. https://0-doi-org.brum.beds.ac.uk/10.3390/app8081357
Chicago/Turabian StyleYuan, Yang, Shilun Feng, Md Eshrat E Alahi, Anindya Nag, Nasrin Afsarimanesh, Hong Zhang, and Shan He. 2018. "Development of an Internet of Things Based Electrochemical Microfluidic System for Free Calcium Detection" Applied Sciences 8, no. 8: 1357. https://0-doi-org.brum.beds.ac.uk/10.3390/app8081357